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Download 1290 Infinity II Evaporative Light Scattering Detector User Manual
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7 Troubleshooting and Diagnostics General Problems Spiky peak tops but flat baseline Probable cause Suggested actions 1 Inconsistent nebulization Nitrogen is the recommended gas - others can be used but may not nebulize as efficiently 2 Incorrect gas being used Change gas to nitrogen or evaluate different nitrogen sources 3 Poor regulation of inlet gases • If using bottled gas, check that gas regulator is functioning correctly and giving consistent flow • Alternatively, use a pulse dampener 4 Insufficient smoothing The broader the peaks, the higher the smoothing value is required. Increase smoothing to 50 for Flash separations 5 Sample precipitation during nebulization • Reduce sample concentration or inject same loading in larger volume • Check sample solubility in mobile phase eluents 6 Inconsistent pump flow rates See remedy for pump pulsation in the baseline noise section Large Baseline offset Probable cause Suggested actions 1 Inefficient evaporation Increase the evaporator temperature and/or gas flow 2 High concentration of non-volatile buffer or Use a lower concentration of stabiliser, unstabilised solvent or a more volatile buffer (ammonium acetate or ammonium formate) stabiliser 78 3 Contaminated diffuser Perform cleaning procedure 4 Optics Heater failed Refer to local distributor or Agilent Technologies Agilent 1290 Infinity II ELSD User Manual
