Download For Reference Purpose Only!
Transcript
CircuLex Human DJ-1/PARK7 ELISA Kit User’s Manual For Research Use Only, Not for use in diagnostic procedures 2. Precision On ly! TM Intra-assay Precision (Precision within an assay) Three samples* of known concentration were tested six times on one plate to assess intra-assay precision. • Intra-assay (Run-to-Run, n=6), CV=3.6 – 6.2% * Samples: cell lysates Human DJ-1/PARK7 concentration (ng/mL) 1,012 970 1,031 985 939 952 1,031 939 982 35.1 3.6% 1,048 1,030 999 1,042 923 1,091 1,091 923 1,022 56.9 5.6% 1,838 1,573 1,631 1,759 1,606 1,622 1,838 1,573 1,671 103.5 6.2% Pu 1 2 3 4 5 6 MAX. MIN. MEAN S.D. C.V. rp os e Sample 1 Sample 2 Sample 3 ce Inter-assay Precision (Precision between assays) Three samples* of known concentration were tested in three separate assays to assess inter-assay precision. • Inter-assay (Run-to-Run, n=3), CV=3.3 – 8.9% * Samples: cell lysates en Human DJ-1/PARK7 concentration (ng/mL) Sample 1 Sample 2 Sample 3 rR ef er 1 2 3 MAX. MIN. MEAN S.D. C.V. Fo Cat#: CY-9050 923 1,024 1,064 1,064 923 1,004 72.9 7.3% 953 1,045 1,139 1,139 953 1,046 92.7 8.9% 13 1,735 1,799 1,840 1,840 1,735 1,791 52.9 3.3% Version#: 130326