Download For Reference Purpose Only!

Transcript
CircuLex
Human DJ-1/PARK7 ELISA Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
2. Precision
On
ly!
TM
Intra-assay Precision (Precision within an assay)
Three samples* of known concentration were tested six times on one plate to assess intra-assay
precision.
• Intra-assay (Run-to-Run, n=6), CV=3.6 – 6.2%
* Samples: cell lysates
Human DJ-1/PARK7 concentration (ng/mL)
1,012
970
1,031
985
939
952
1,031
939
982
35.1
3.6%
1,048
1,030
999
1,042
923
1,091
1,091
923
1,022
56.9
5.6%
1,838
1,573
1,631
1,759
1,606
1,622
1,838
1,573
1,671
103.5
6.2%
Pu
1
2
3
4
5
6
MAX.
MIN.
MEAN
S.D.
C.V.
rp
os
e
Sample 1 Sample 2 Sample 3
ce
Inter-assay Precision (Precision between assays)
Three samples* of known concentration were tested in three separate assays to assess inter-assay
precision.
• Inter-assay (Run-to-Run, n=3), CV=3.3 – 8.9%
* Samples: cell lysates
en
Human DJ-1/PARK7 concentration (ng/mL)
Sample 1 Sample 2 Sample 3
rR
ef
er
1
2
3
MAX.
MIN.
MEAN
S.D.
C.V.
Fo
Cat#: CY-9050
923
1,024
1,064
1,064
923
1,004
72.9
7.3%
953
1,045
1,139
1,139
953
1,046
92.7
8.9%
13
1,735
1,799
1,840
1,840
1,735
1,791
52.9
3.3%
Version#: 130326