Download Tribute User Manual - Protein Technologies, Inc.

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g. Reaction Ratios AA:Act:Base – Software calculates suggested
amino acid to activator to base ratios. Click the button to change
this value
The Base section lists:
a. Base Name – Click the button to change the name of the base
b. Base Mole Wt – Click the button to change the molecular weight of
the base (in g/mol)
c. Base Density – Click the button to change the density of the base
(g/mL)
d. Base Total Volume (est) – Software calculates the suggested total
volume of base solution (in mL) to use for the synthesis. Click the
button to change this value.
e. Base Weight – Software calculates amount of base (in g) to create
the base solution.
f. Base Volume – Software calculates the volume of base (in mL) to
create the base solution.
At the bottom of the screen, the Print button allows you to print the
calculated values. The Return button returns you to the Sequence Editor
screen.
7. Copy Fwd – Copies the programs assigned to the selected cycle (in all 4
columns) through to the last cycle.
8. Go To Pos – Select cycle position to edit
9. First – Goes to first cycle
10. Prev – Goes to previous cycle
11. Next – Goes to next cycle
12. Last – Goes to last cycle
13. Syn RVs – Select 1 or 2
14. Load RV1 – Loads current peptide settings onto RV 1 on Load Synthesis
screen
15. Load RV2 – Loads current peptide settings onto RV 2 on Load Synthesis
screen
16. Clear All Loaded RVs – Clears all settings on the Load Synthesis screen
17. Print – Prints the values on the Sequence Calculations screen
18. Save – Saves settings
19. Save As – Saves settings to a different file name
20. Cancel - Main Menu – Returns to Main Menu without saving changes
3.2.1 Calculate Coupling Difficulty
The Sequence Editor may be used to calculate the synthesis difficulty of each
coupling step for a peptide sequence.
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