Download Manual: Phyto-PAM

CHAPTER 4 FEATURES OF THE SOFTWARE PHYTOWIN
all types of phytoplankton than the average of the former. For
example, for cyanobacteria the actinic effect of 470 nm is very low.
Therefore, as Yield or ETR are plotted against incident PAR (and not
absorbed PAR, which is different for the various types of
phytoplankton) there may be a relatively large decline in Yield, when
actinic illumination is increased from the level of high frequency
Measuring Light (e.g. MF128) to a mixture of MF128 plus red
Actinic Light. This effect is particularly pronounced with low light
adapted or stressed samples, which already are limited by dark
enzymatic reaction at MF128 (i.e. ca. 20 µmol quanta m-2 s-1).
The illumination period at each step is defined by
Time/10s. When "Uniform time" is activated, the
same time can be entered for all steps by typing a new value for one
step and confirmation by mouse-click.
A Light Curve recording does not necessarily have to involve 20
illumination steps. When at a particular step under Time/10s a 0
(zero) is entered, the Light Curve is terminated with the preceding
step.
Alternatively, it is also possible to record up-down Light
Curves with light intensity first increasing to saturation and then
decreasing again. The observed hysteresis pattern provides
information on light activation parameters.
Light Curve recordings may be started repetitively with the help
of the Clock-function (see 4.1). In this way, changes in the light
adaptation state and the physiological health of a sample can be
followed over longer periods of time.
The Light Curve Parameters can be saved in lcp-files
and reinstalled at any time by opening the
corresponding file. This provides great flexibility in Light Curve
recordings. An lcp-file applies to a particular type of Measuring
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