Download Sequence Capture Lab Procedure

(around 38 µL)
i) Make a fresh dilution of 80% ethanol.!
Take 40mL of 99.5% ethanol and 10mL
milliQH2O (Extraction room).
ii) Transfer your DNA samples from the PCR
strip to easy-cap tubes (the magnetic stand
can only have ependorf tubes)
The shortest fragments (<400bp) are discarded with the supernatant.
100
iii) Start with step 1.
Put samples in fume hood (turn on) for faster drying.
25
In the drawer
under bench
50
The beads capture fragments >600 bp.
15
30
easy-cap tubes
(around 72 µL)
While waiting you
can prepare the
new tubes with
23 µL of AMPure
XP beads.
easy-cap tubes
The supernatant now consists of 400-600 bp long fragments
Keep samples on the stand while removing supernatant.
100
38.5
The beads now capture the 400-600 bp long fragments
Put samples in fume hood (turn on) for faster drying.
25
23
easy-cap tubes
(around 58 µL)
The beads capture fragments from 400bp and above.
100
16.5
easy-cap tubes
easy-cap tubes
Put samples in fume hood (turn on) for faster drying.