Download ACQUITY UPLC H-Class System Guide

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Flexibility in solvent mixing by using a quaternary solvent manager
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A flow-through-needle sample manager that facilitates the transfer of
HPLC methods to UPLC.
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Pump and sample manager design enhancements to minimize
dispersion and reduce cycle time
Flow-through-needle injector
The sample manager in the ACQUITY UPLC H-Class system uses a
flow-through-needle mechanism that differs radically from the loop-based
injector used by the ACQUITY UPLC system. The flow-through-needle
mechanism aspirates a sample and holds it in the sample needle in
preparation for injecting the sample onto the column. The needle serves as
part of the injection flow path when the sample is pushed onto the column.
Using the flow-through-needle mechanism, the system operates similarly to
most traditional HPLC systems, facilitating the transfer of HPLC methods
with its familiar operation. The flow-through-needle mechanism also does not
require you to learn new injection modes, improves injection accuracy, and
decreases cycle time for small volume injections. Gradients pass through the
needle during injection, ensuring complete sample recovery.
Wash solvent
The wash system uses a single solvent to clean the outside of the sample
needle and prime the wash system. The wash solvent does not enter the
injection flow path.
Purge solvent
The primary function of the purge solvent is to move sample along the
injection pathway. The purge solvent also primes the sample syringe and
injection pathway. It is injected onto the column only during auto-dilution,
when it is used as the dilution solvent.
Active solvent conditioning
HPLC and UPLC applications benefit from additional pre-column,
mobile-phase heating to improve chromatographic separations. The
ACQUITY UPLC H-Class column heater uses an active preheater to condition
solvent as it enters the column. The active preheater is a heat source that
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ACQUITY UPLC H-Class System