Download Manual - Nuclear Sciences and Applications

FAO/IAEA INTERREGIONAL TRAINING COURSE ON MUTANT
GERMPLASM CHARACTERISATION
PRIMER DESIGN
In many cases (such as TILLING/Ecotilling) it is best to be searching for potentially
functional variation. So, it will be more efficient to screen exonic regions. In this example
notice the exonic regions are mostly on the left side.
It is not very intuitive how to get bot the genomic and transcript sequence from this graphical
output. Put your mouse over the transcript and right click. A new window will appear from
phytozome and you can get the sequences you need from the sequencing tab.
FOR TILLING and Ecotilling applications design primers following protocol in chapter
section 13.2.1.
Pa g e | 5-2