Download Manual - Nuclear Sciences and Applications

FAO/IAEA INTERREGIONAL TRAINING COURSE ON MUTANT
GERMPLASM CHARACTERISATION
ISSR-20
ISSR-21
ISSR-22
ISSR-23
ISSR-24
ISSR-25
ISSR-26
(CA)8 A
(CA)8 G
(GT)8 A
(GT)8 C
(GT)8 T
(TC)8 A
(GT)8 C
ISSR-46
ISSR-47
ISSR-48
ISSR-49
ISSR-50
ISSR-51
ISSR-52
ISSR
(TG)8 RC
(ACC)6
(ATG)8
(CTC)6
(GAA)6
(GACA)6
(TCC)5 RY
Y=C/T
R=A/G
8.3. References
Davila, J. A., Y. Loarce, and E. Ferrer, 1999. Molecular characterization and genetic mapping of
random amplified microsatellite polymorphism in barley. Theor.Appl.Genet. 98: 265-273
Provan, J., W. T. B. Thomas, B. P. Forster, and W. Powell, 1999. Copia-SSR: a simple marker
technique which can be used on total genomic DNA. Genome. 42: 363-366
Yang, W., A. C. De Olivera, I. Godwin, K Schertz, and J. L. Bennetzen, 1996. Comparison of
DNA marker technologies in characterizing plant genome diversity: variability in Chinese
sorghums. Crop Sci. 36: 1669-1676
Zietkiewicz, E., A. Rafalski, and D. Labuda, 1994. Genome fingerprinting by simple sequence
repeat (SSR)-anchored Polymerase Chain Reaction Amplification. Genomics. 20: 176-183
8.4. Reagents needed
Use only sterile distilled water for all solutions:
- Taq buffer
- dNTPs
- Sterile distilled water
- Primer(s)
- Taq DNA polymerase (5U/l)
- DNA (10-20 ng/l)
- 10 x loading buffer
Glycerol (80%)
600 l
Xylene cyanol
2.5 mg
Bromophenol blue
2.5 mg
Water
400 l
- 5 x loading buffer
Glycerol (80%)
300 l
Xylene cyanol
2.5 mg
Bromophenol blue
2.5 mg
Water
400 l
- Ethidium bromide
Pa g e | 8-4