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Gubbs Mass Spec Utilities 2.x.x User Manual Figure 5 Page 10 of 40 MRM Generator Utility 6.1 Choose a SubProject 6.2 Choose an Assay Request File Note: Users may view the Assay Request File by clicking the View File button 6.3 Modify the Acceptance Criteria as needed • • 6.4 Min Daughter m/z: The minimum daughter ion m/z that will be considered when evaluating mass spectra Min Daughter Intensity: Ions must be greater than this value in order to be considered as acceptable Click on Populate Form If the user wishes to populate the form with information from a different Assay Request file, the Clear Form button must first be clicked. Version 6.0 (July 20, 2006) C:\GubbsIncApps\GMSU\UserManuals\UserManual\GMSU_UserManual_06.doc