Download pCDF vectors - System Biosciences

pCDF cDNA Expression Lentivectors
Cat. #s CD100A-1 – CD111B-1
Contents
I.
Introduction and Background
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Purpose of this Manual
Advantages of the Lentivector Expression System
pCDF cDNA Cloning and Expression Lentivectors
List of Components
Additional Required Materials
Safety Guidelines
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II. Protocol
A. cDNA Amplification
B. Preparation of Digested pCDF Vectors
C. cDNA Cloning into pCDF Vectors
D. Packaging of pCDF Expression Construct
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III. Troubleshooting
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A. Large number of colonies on control plate
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B. No or low number of colonies on plate with cDNA sample 12
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C. No correct cDNA inserts
IV. References
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V. Appendix
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G.
Map and Features for pCDF1-MCS1 Vector
Map and Features for pCDF1-MCS2-EF1-Puro Vector
Map and Features for pCDF1-MCS2-EF1-copGFP Vector
Restriction Maps of Multiple Cloning Sites (MCS)
Properties of copGFP Fluorescent Protein
Related Products
Technical Support
VI. Licensing and Warranty Statement
888-266-5066 (Toll Free)
650-968-2200 (outside US)
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