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was left overnight to dry after cleaning, was carried out three times during the cruise (at the
ice, at Marion and one day outside of PE).
Filtered / unfitered
BB9
The WetLabs BB9 instrument provides a backscatter measurement at 9 different
wavelengths. The instrument was set up in underway mode, in a large volume black bin as
the flow chamber with continual ships underway water supply. The residual time for sample
water in the flow chamber is unknown but ought to be determined to assist with post cruise
data processing and binning. The instrument was set up to record data for two minutes at 10
minute intervals. The large volume needed to fill the BB9 flow chamber >50L made it
impossible to sample backscattering measurements from the three CTD depths.
Acidified / non acidified
Fiptered milli-q?? need to dokl
4.10 Coulter Counter
Written by Emma Bone
Key Participants: Emma Bine, Sandy Thomalla, Natasha Horsten

The instrument was set up as per standard protocol

Electrolyte was generated by first filtering seawater through a 0.8um polycarbonate
filter/ or Whatman GF/F, followed by filtering through a 0.2um isopore polycarbonate
filter.

The 100um aperture tube was inserted and calibrated using 14 drops of 20um beads

During the initial test runs the machine failed to analyse the correct volume of sample
(40ml for 20 runs). It was soon after discovered that the Accuvette tube was not
sealing properly and filtered seawater was being mistakenly acquired from the
electrolyte jar. The problem was appeased upon fixing the seal.

A specific SOP was created for the SOSCEx cruise, which standardly made use of the
100um aperture, sampling 20 runs at 2ml per run. At one stage the number of runs