Download Human Bone Metabolism Array 2
Transcript
Cytokine Detected BMP-2, BMP-6, BMP-7, DKK-1, MMP-3, OPG, OPN, (10) PDGF-BB, TGF3, TRANCE Format One standard glass slide is spotted with 16 wells of identical cytokine antibody arrays. Each antibody is arrayed in quadruplicate. Detection Method Fluorescence with laser scanner: Cy3 equivalent dye Sample Volume 50 – 100 l per array Reproducibility CV <20% Assay duration 6 hrs See Section V For Array Map Fluor dye (cy3 equivalent) B Biotin-Streptavidin complex Detect antibody Cytokine Capture antibody Glass Slide Support Quantibody Human Bone Metabolism Array 2 1