Download Human Bone Metabolism Array 2

Transcript
Cytokine Detected BMP-2, BMP-6, BMP-7, DKK-1, MMP-3, OPG, OPN,
(10)
PDGF-BB, TGF3, TRANCE
Format
One standard glass slide is spotted with 16 wells of
identical cytokine antibody arrays. Each antibody is
arrayed in quadruplicate.
Detection Method
Fluorescence with laser scanner: Cy3 equivalent dye
Sample Volume
50 – 100 l per array
Reproducibility
CV <20%
Assay duration
6 hrs
See Section V
For Array Map
Fluor dye (cy3 equivalent)
B
Biotin-Streptavidin complex
Detect antibody
Cytokine
Capture antibody
Glass Slide Support
Quantibody Human Bone Metabolism Array 2
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