Download NanoDrop ND-1000

NanoDrop® ND-1000 Spectrophotometer User’s Manual V3.0.1
rev 16-Apr-04
1 Overview
1.1
Instrument Description
The NanoDrop® ND-1000 is a full-spectrum (220-750nm) spectrophotometer that measures 1 ul
samples with high accuracy and reproducibility. It utilizes a patented sample retention technology
that uses surface tension alone to hold the sample in place. This eliminates the need for
cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In
addition, the ND-1000 has the capability to measure highly concentrated samples without dilution
(75X higher concentration than the samples measured by a standard cuvette spectrophotometer).
1.2
Operation
A 1 ul sample is pipetted onto the end of a fiber optic cable (the receiving fiber). A second fiber optic
cable (the source fiber) is then brought into contact with the liquid sample causing the liquid to bridge
the gap between the fiber optic ends. The gap is controlled to a 1mm path. A pulsed xenon flash
lamp provides the light source and a spectrometer utilizing a linear CCD array is used to analyze the
light after passing through the sample. The instrument is controlled by special software run from a
PC, and the data is logged in an archive file on the PC.
1.3
Applications
UV/VIS spectrophotometry is simple for samples as small as 1 ul using the NanoDrop® ND-1000
Spectrophotometer. The small sample requirement and ease of use make the NanoDrop® ND-1000
Spectrophotometer ideally suited for measuring:
• Nucleic acid concentration and quality of nucleic acid samples up to 3700 ng/ul (dsDNA)
without dilution
• Fluorescent dye labeling density of nucleic acid microarray samples
• Purified protein analysis (A280) up to 100 mg/ml (BSA)
• Bradford Assay analysis of protein
• BCA Assay analysis of protein
• Cell density measurements
• General UV-Vis spectrophotometry
4