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Download PowerPlex® 18D System
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TMD031.0514:TMD031.0514 5/28/2014 12:18 PM Page 1 PowerPlex® 18D System All technical literature is available on the Internet at: www.promega.com/protocols/ Please visit the web site to verify that you are using the most current version of this Technical Manual. Please contact Promega Technical Services if you have questions on use of this system. E-mail: [email protected] 1. Description..................................................................................................................................2 2. Product Components and Storage Conditions ....................................................................3 3. Before You Begin .......................................................................................................................5 A. Precautions ........................................................................................................................5 B. Spectral Calibration .........................................................................................................5 4. Protocols for Direct Amplification Using the PowerPlex® 18D System ........................6 A. Direct Amplification of DNA from Storage Card Punches.......................................6 B. Direct Amplification of DNA from Swabs.................................................................10 5. Instrument Setup and Sample Preparation........................................................................13 A. Detection of Amplified Fragments Using the Applied Biosystems® 3500 or 3500xL Genetic Analyzer..........................................13 B. Detection of Amplified Fragments Using the ABI PRISM® 3100 or 3100-Avant Genetic Analyzer with Data Collection Software, Version 2.0, or the Applied Biosystems® 3130 or 3130xl Genetic Analyzer with Data Collection Software, Version 3.0 ...............................23 6. Data Analysis ...........................................................................................................................26 A. PowerPlex® 18D Panels, Bins and Stutter Text Files with GeneMapper ® ID-X Software, Version 1.2.................................................................26 B. Creating a Size Standard with GeneMapper ® ID-X Software, Version 1.2...........27 C. Importing the CC5 ILS 500 IDX Size Standard into GeneMapper ® ID-X Software, Version 1.2.................................................................29 D. Creating a Databasing or Paternity Analysis Method with GeneMapper ® ID-X Software, Version 1.2........................................................29 E. Creating a No-Global-Filter Analysis Method with GeneMapper ® ID-X Software, Version 1.2.................................................................32 F. PowerPlex® 18D Panels and Bins Text Files with GeneMapper ® ID Software, Version 3.2.....................................................................35 G. Creating a Size Standard with GeneMapper ® ID Software, Version 3.2...............36 H. Importing the CC5 ILS 500 Size Standard into GeneMapper® ID Software, Version 3.2 .....................................................................37 I. Creating a Databasing or Paternity Analysis Method with GeneMapper ® ID Software, Version 3.2............................................................38 J. Creating a No-Global-Filter Analysis Method with GeneMapper ® ID Software, Version 3.2.....................................................................40 K. Controls ...........................................................................................................................43 L. Results..............................................................................................................................43 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Revised 5/14 Part# TMD031 Page 1
