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Protocol
2
Add 10 µl gDNA at 5 ng/µl (50 ng total) to each well of the new 96-well MIDI plate
labeled NLT.
3
Add 25 µl Tagment DNA Buffer to each well of the NLT plate.
4
Add 5 µl Tagment DNA Enzyme 1 to each well of the NLT plate.
5
Add 10 µl PCR-grade water to each well of the NLT plate.
6
Mix thoroughly as follows:
a Seal the NLT plate with a Microseal ‘B’ adhesive seal.
b Shake the NLT plate on a microplate shaker at 1800 rpm for 1 minute.
7
Centrifuge the NLT plate to 280 × g for 1 minute.
8
Place the sealed NLT plate on the pre-heated microheating system. Close the lid and
incubate at 58°C for 10 minutes.
9
Remove the NLT plate from the microheating system.
10 Remove the adhesive seal from the NLT plate.
11 Add 15 µl Stop Tagment Buffer to each well of the NLT plate.
12 Mix thoroughly as follows:
a Seal the NLT plate with a Microseal ‘B’ adhesive seal.
b Shake the NLT plate on a microplate shaker at 1800 rpm for 1 minute.
13 Centrifuge the NLT plate to 280 × g for 1 minute.
14 Incubate the NLT plate at room temperature for 4 minutes.
15 Proceed to Clean Up Tagmented DNA on page 13.
12
Part # 15046431 Rev. A