Download Sample & Assay Technologies PyroMark® Q24 Software User Guide

Histogram
Histogram showing a theoretical CpG assay result.
When setting up an AQ or CpG assay, the theoretical representation of the
expected Pyrosequencing® peak pattern is presented in the “Histogram” area.
The following icons and colors are used in the histogram:
„
Variable positions are highlighted with a blue-gray background color
„
When showing reference peaks, blue diamonds are displayed above the
reference peaks
„
Bisulfite treatment controls are highlighted with a yellow background color.
When showing reference peaks, orange diamonds are displayed above the
bisulfite treatment controls (CpG assays only).
Zoom histogram
It is possible to zoom in on the histogram by selecting a stretch of it with the left
mouse button.
Zoom out either by right-clicking the histogram area and selecting “Zoom Out”
from the context menu (the zoom is set to the previous level) or by doubleclicking the histogram area (the zoom is set to 100%).
Export the histogram as an image
The histogram can be copied as an image to the clipboard by right-clicking the
histogram and selecting “Copy as Image” from the context menu. The image
can be pasted into applications that support Enhanced Metafile (EMF) images.
PyroMark Q24 Software User Guide 07/2009
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