Download Protocol - Norgen Biotek Corp.

Transcript
B. Purification of Large RNA, gDNA, microRNA and Proteins
Lyse cells or tissue using Buffer SK
Bind microRNA to microRNA
Enrichment Column
Bind large RNA and
DNA to column
SPIN
Wash
Flowthrough
(Proteins and
microRNA)
Wash
Flowthrough
(Proteins)
SPIN
SPIN
SPIN
Elute microRNA
Elute Large
RNA
SPIN
SPIN
Large
RNA
Bind Proteins to
Initial Column
Wash DNA
SPIN
SPIN
Wash
Elute gDNA
SPIN
microRNA
gDNA
SPIN
Elute Proteins
SPIN
Proteins
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