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Image optimization
Choosing Range Indicator
• In the Dimensions view control tab check the
Range Indicator box (Fig. 49).
• The scanned image appears in a false-color
presentation (Fig. 50). If the image is too bright,
it appears red on the screen. Red = saturation
(maximum). If the image is not bright enough, it
appears blue on the screen. Blue = zero
(minimum).
Fig. 49
Dimensions view control tab for a
PALM image
Fig. 50
Image with Range Indicator
Adjusting the laser intensity
• When the image is saturated with the slider in
the Display view control tab at Reset (linear),
reduce AOTF transmission in the Laser control
section of the Channels tool (Fig. 51).
Adjusting gain
Fig. 51
Display view control tab
• Reduce the EMCCD Gain until the red pixels
just disappear.
Adjusting display settings
• If the slider in the Display view control tab is offset from the Reset (linear) position, red as well as blue
pixels can also occur. Use the slider to set contrast and brightness to values that avoid red and blue
pixels. (Fig. 51).
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