Download Assay Value Sheet

Stains and Dyes – RetiCount™
Ordering Information
Product
Product Number
Packaging
Reticount™
3774
30 ml
Introduction
J.T.Baker® Reticount™ is a special hematology stain intended
for the identification of reticulocytes in blood smears
and for counting the number of reticulocytes per 1000
erythrocytes by microscopic analysis. An erythrocyte will
be counted as reticulocyte when it contains 2 or more
blue-stained granules.
Composition
RetiCount™ is water based and contains potassium oxalate,
sodium chloride and brilliant cresyl blue.
Stability and Storage
RetiCount™ is stable for 2 years when the bottles are
kept closed and stored at room temperature (18 - 30 °C).
Products that are past their shelf-life must be disposed of,
according to local disposal guidelines.
Procedure
• Single slide dip technique:
1.Mix in a plastic test tube 1 drop of RetiCount™ with 3
drops of fresh whole human blood (anticoagulated
with EDTA or citrate). Heparin interferes with
RetiCount™ and should not be used.
2.Incubate 15 minutes at room temperature.
3.Mix the content after incubation.
4.Make a smear of this mixture on a slide.
5.Air dry the smear before microscopic examination.
6.Examine the object at a microscopic magnification of
1000 (10x ocular and 100x oil immersion objective).
7.The erythrocytes are homogeneous pale blue
coloured. A reticulocyte contains 2 or more bluestained particles.
8.Calculate the reticulocyte percentage according to
laboratory procedures.
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