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Download Circulating DNA from Plasma
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Circulating DNA from Plasma Protocol-at-a-glance (Rev. 02) NucleoSpin® Plasma XS 1 1a High Sensitivity protocol Rapid protocol Use up to 240 µl plasma Use up to 200 µl plasma Prepare sample Optional: Proteinase K treatment Add 20 µl Proteinase K / Mix Incubate at 37 °C for 10 min 2 3 4 Adjust binding conditions Mix sample Bind DNA Add 360 µl BB Add 300 µl BB Invert tube 3 x Invert tube 3 x Vortex 3 s Vortex 3 s Spin down briefly Spin down briefly Load lysate Load lysate 30 s 2,000 x g 30 s 11,000 x g 5s 11,000 x g 5 Wash and dry silica membrane 1st wash 500 µl WB 1st wash 500 µl WB 30 s 11,000 x g 2nd wash 250 µl WB 30 s 11,000 x g 2nd wash 250 µl WB 3 min 11,000 x g 6 7 Elute DNA Removal of residual ethanol 3 min 11,000 x g 20 µl Elution Buffer 20 µl Elution Buffer 30 s 11,000 x g 30 s 11,000 x g 8 min 90 °C / MACHEREY-NAGEL GmbH & Co. KG • Neumann-Neander-Str. 6-8 • D-52355 Düren • Germany Tel.: +49 (0) 24 21 969 270 • www.mn-net.com • e-mail: [email protected] MN
