Download Single Molecule Light Microscopy ImageJ Plugins

GDSC SMLM ImageJ Plugins
Illustration 15: Trace Molecules dialogue
The following parameters can be configured:
Parameter
Description
INPUT
Specify the localisations to use
DISTANCE THRESHOLD (NM) Maximum distance (in nm) for two localisations to belong to the
same trace
DISTANCE EXCLUSION (NM)
Exclusion distance (in nm) where no other localisations are
allowed. Use this setting to be sure that a trace links together
localisations that are not close to any other localisations.
Ignored if less than the distance threshold
TIME THRESHOLD
(SECONDS)
Maximum distance in seconds for two localisations to belong to
the same trace (should cover a minimum of 1 frame)
TRACE MODE
LATESTFORERUNNER - Search from the closest time distance in the
past for earlier localisations of the same fluorophore. This is the
best mode for moving molecules
EARLIESTFORERUNNER - Search from the maximum time distance in
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