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Positive for Mycobacterium tuberculosis complex Red-purple colour appears in both the T and C reading area. The result is read as positive if area T shows red-purple colour that is lighter than or the same as, or darker than the colour in area C. Negative for Mycobacterium tuberculosis complex A test is negative if red-purple colour appears in area C but not in area T. Invalid test A test is invalid if red-purple colour does not appear in area C. If a test is invalid, repeat test using a new test plate, preferably from a newly opened foil pouch. If the repeated test result is valid, enter this result on the case report form. 53