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WA R N I N G : D O N O T U S E O T H E R E M B R Y O
CATHETER MODELS WITHOUT CHECKING THAT
THEY FIT IN THE DOCKING POSITION
APPROPRIATELY AND DO NOT TOUCH THE
BOTTOM OF THE MICROCHAMBER]


Indicator slots aligned : closed position
Note : In the closed position
the indicator slot in the lid is
aligned with an identical slot
located on the rim of the inner
chamber (see Figure 3a)
Powder-free gloves
INVO Bioscience INVO holding block for the inner
chamber (sold separately)
The specially designed holding block accessory acts as a passive
thermal mass while physically stabilizing the inner chamber
temperature during various steps of the p r o c e s s i n c l u d i n g
microscopic visualization of embryos. This block is provided
not sterile and should be cleaned to the standard of the
microscope base and microscope per the laboratory procedure .
Turn to open
Figure 3b : Top view of lid in open position
Lid opening
WARNING : IN THE INVO PROCEDURE, EMBRYO
DEVELOPMENT IS FIRST EVALUATED AT THE END
OF THE INCUBATION PERIOD WHICH IS 48-72
HOURS POST FERTILIZATION. THEREFORE ANY
ABNORMALITIES THAT WOULD HAVE BEEN
DETECTED AT AN EARLIER STAGE (PRO-NUCLEI
STAGE) MAY NO LONGER BE APPARENT WHEN THE
EMBRYOS ARE EVALUATED FOR TRANSFER. AS A
RESULT THERE MAY BE INCREASED RISK THAT AN
ABNORMAL ( E.G.TRIPLOID) EMBRYO C O U L D B E
TRANSFERRED TO THE UTERUS.
WARNING : THE INVOcell IS A SINGLE USE DEVICE.
REUSE MAY RESULT IN CONTAMINATION.
WA R N I N G : D O N O T R E S T E R I L I Z E .
RESTERILIZATION MAY RESULT IN FAILURE OF
THE INVOcell .
WARNING : UTILIZE A CULTURE MEDIUM THAT
WILL SUPPORT CONTINUED E M B R Y O N I C
DEVELOPMENT FOR UP TO 3 DAYS.
c. Turn the lid counter clock-wise to open (see gure 3).
Rinse the inner chamber with pre-warmed culture
medium using an adjustable volume pipette to add and
remove medium through the circular opening in the lid
(see Figure 4)
d. the volume of the inner chamber is 1.08 ml. Using a
sterile glass pipette or a pipette with a long thin plastic
tip , ll the inner chamber with pre-warmed culture
medium.
FIGURE 4 : FILLING THE INNER CHAMBER
WARNING : DO NOT INCUBATE BEYOND 3 DAYS
WARNING : DO NOT USE IF PRODUCT OR PACKAGE
APPEARS DAMAGED
INSTRUCTIONS FOR USE :
1. Pre-warm the following in a 37°C incubator for at least 1 hour
prior to use :

Unopened package (1 of 2) containing the inner
chamber

Unopened package (2 of 2) containing outer rigid shell
for the inner chamber

INVO Bioscience INVO holding block

New unopened bottle of cell culture medium
2. Placement of gametes ( sperm and oocyte ) :
a. Open the pre-warmed package (1 of 2) containing the
inner chamber.
b. Place the closed inner chamber in the pre-warmed
holding block and keep the outer rigid shell in its
package at 37°C.
Note : The inner chamber is pouched in the closed condition
(see Figure 3a).
Figure 3a : Top view of lid in closed position
Pipette lled
with culture
medium
Inner
chamber in
holding block
e. Close the inner chamber and aspirate the culture
medium in excess in the overow well. Turn the inner
chamber upside down to verify the absence of major air
bubbles. If necessary eliminate any trapped air bubbles
lling the micro-chamber .
f. Reopen the chamber
g. Using an adjustable volume pipette with a 0-200 ml tip,
transfer 30,000 motile sperm into the inner chamber.
The added volume is generally under 50 µl.
h. Using a glass pipette or an adjustable volume pipette
with a 250-1000 µl tip, transfer oocytes into the inner
chamber. The added volume should not exceed 50 µl.