Download Nucleic Acid Full User Guide

1. Turn on background box by clicking on Local Background in the Show group. This will create an
additional box around your band that shows you where the background is being sampled from.
2. Select the appropriate background method from the drop-down menu:
a. None – Does not subtract a background value (band signal and other quantification values that
depend on signal will have a value of NaN, meaning undefined).
b. Lane – Subtracts the background pixel values in the lane. This is the recommended background
methods for DNA Gels. This method is good for well-defined bands.
c. User-Defined – Assign a shape to determine the background (if region is undefined, band signal
and other quantification values that depend on signal will have a value of NaN, meaning
undefined).
d. Average or Median – Subtracts the average or median of pixel values in the selected
background segments.
i. Border Width – You can change the border width of the local background box anywhere
from 1 to 5 pixels.
ii. All – Use all boundary segments.
iii. Top/Bottom – Use top/bottom segments for average or median. This is useful if you
have very small spaces between lanes or very intense bands resulting in small spaces
between adjacent bands.
iv. Right/Left – Use right/left segments for average or median. This is useful if you have a
lot of spurious bands you won’t be quantifying.
F. Editing DNA Gel Lane Information
The DNA Gel Lanes Table contains default columns that are pertinent to DNA Gel Lanes. Additional columns
are available in the Table ribbon at the top of the window.
1. Click the DNA Gel Lanes tab at the lower left of the table to display the DNA Gel Lanes table.
2. The Lane Name column contains a default name for each lane, e.g., L01, L02, etc. You can edit a name
by selecting the text in the Lane Name field and typing over the selected text with another name. The
name you enter in the field will appear in the Profile title, the Single Image view, and in any report
using the Lane Name field.
Table ribbon
G. Obtaining Band Size and other Band Quantification Results
The DNA Gel Bands Table contains a number of default columns that are pertinent to DNA Gel Bands such as
band size and band signal strength. Additional columns are available in the Table ribbon at the top of the
window. Click the DNA Gel Bands at the lower left of the table to display the DNA Gel Bands table and see
your results.
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