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CaMKK beta Kinase Assay Kit
User’s Manual
For Research Use Only, Not for use in diagnostic procedures
2. Add protein A agarose beads (20 µL of 50% bead slurry). Incubate with gentle rocking for 1–3
hours at 4°C.
3. Microcentrifuge for 30 seconds at 4°C. Wash the protein A agarose beads 3 times with 500 µL of
Cell Lysis Buffer and successively once with Kinase Buffer. Keep on ice during washes.
4. Resuspend the protein A agarose beads with 20-40 µL of Kinase Buffer and use 10 µL as an
enzyme sample* to measure CaMKKβ activity according to the procedure in the step 3-12 of
“Standard Assay” on page 9.
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* Please take care to transfer the protein A agarose beads to the well of the Antibody-coated
Microplate as little as possible.
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Cat#: CY-1185
14
Version#: 141107