Download AssayMaxTM Human Complement C8 ELISA Kit

Performance Characteristics



The minimum detectable dose of C8 as calculated by 2SD from the mean
of a zero standard was established to be 0.4 ng/ml.
Intra-assay precision was determined by testing replicates of three
plasma samples in one assay.
Inter-assay precision was determined by testing three plasma samples in
twenty assays.
Sample
n
CV (%)
Average
CV (%)
Intra-Assay Precision
1
2
3
20
20
20
4.6%
4.3 %
3.9%
Inter-Assay Precision
1
2
3
20
20
20
7.5%
8.1%
7.2%
4.3%
7.6%
Recovery
Standard Added Value
Recovery %
Average Recovery %
1.5 – 15 ng/ml
88 – 111%
103%
Linearity

Plasma and serum samples were serially-diluted to test for linearity.
Average Percentage of Expected Value (%)
Sample Dilution
Plasma
Serum
1:5000
96%
94%
1:10000
100%
99%
1:20000
106%
107%
Cross-Reactivity
Species
Canine
Monkey
Mouse
Rat
Swine
Rabbit
Bovine
Proteins
Cross Reactivity (%)
None
<20%
None
1%
None
None
None
Cross Reactivity (%)
7