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Manual Test Mode
2. Watch the display and move the TEVC probe stepwise down by clicking the appropriate arrow
button
, until the membrane potential is reached. You may have to move about 10 steps
for a successful impalement. Make sure not to hit the well bottom or wall. This would crash the
TEVC probe. Move to a new oocyte if the membrane potential cannot be reached.
Each click moves the TEVC probe down another 33 µm. The voltage drops to the membrane
potential (–40 mV for a typical healthy oocyte, but can range from –10 mV to –70 mV ) in the
moment when the oocyte is impaled by the electrodes.
3. Switch to the other electrode and check the membrane potential again to see if this electrode
has also penetrated the cell. Sometimes it may happen that one electrode has penetrated the
cell while the other has not. In this case, repeat step 2 until both electrodes have successfully
impaled the oocyte.
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