Download PureLink Pro Quick96 Plasmid Purification Kit

Before Starting
Introduction
Instructions for preparing the PureLink™ buffer solutions for
plasmid DNA purification are provided below. Prepare the
buffer solutions before beginning the purification.
Materials
Needed

96% Ethanol

Optional: 100% Isopropanol

Optional: Water bath (37oC)
Preparing
Resuspension
Buffer with
RNase A
Prepare the PureLink™ Pro 96 Resuspension Buffer with the
PureLink™ Pro 96 RNase A as described below:
For 4 × 96 preps
1.
Transfer the entire contents of the PureLink™ Pro 96
RNase A bottle into the Resuspension Buffer bottle. Mix
thoroughly.
2.
Mark the label on the Resuspension Buffer to indicate
that RNase A has been added. Store at 4oC.
For 24 × 96 preps
1.
Add 12 mL of PureLink™ Pro 96 RNase A into each
Resuspension Buffer bottle. Mix thoroughly.
2.
Mark the labels on the Resuspension Buffer to indicate
that RNase A has been added. Store at 4oC.
Note: RNase A digestion is performed during sample preparation to
degrade RNA present in the sample and to minimize RNA
contamination in the purified DNA sample. RNA contamination can
inflate the DNA content measured at 260 nm.
Lysis Buffer
Store the Lysis Buffer at room temperature (≥20oC).
The Lysis Buffer contains SDS, which can precipitate out of
the buffer if stored below 20oC. If a precipitate is present,
incubate the Lysis Buffer in a 37oC water bath for 5 minutes,
or until the SDS redissolves and the solution clears.
Avoid shaking the Lysis Buffer, as this can lead to foaming.
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