Download Development of a protein quantification algorithm for data analysis
Transcript
Trapezoid method This method consists in calculate the peak in time area drawing rectangles between two consecutive points and summing the resulting area. This rectangle is drawn using the retention time as the rectangle base and the intensity as the height. In order to fill the existing gaps between the rectangle and the peak in time shape a triangle will be drawn, and the resulting area will be summed as well. Figure 42: Using rectangles and triangles in order to calculate a bell shape area. As can be seen in the previous figure, a rectangle has been formed every two points from which can be calculated the area. arearectangle = base ∗ height = (retT2 − retT1 ) ∗ intensity1 At this point a triangle will be drawn in order to fill the existing gap (shape’s left side) or subtract it from the previously drawn rectangle (shape’s right side). In order to do it, the next formula will be used: base ∗ height 2 (retT2 − retT1 ) ∗ (intensity2 − intensity1 ) = 2 areatriangle = 71
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