Download Data Acquisition and Analysis Software Quick Start Guide

Contents
Chapter 1. Installation __________________________ 3
Chapter 1.1 System Requirements ______________________ 3
Chapter 1.2 Installing the Software ______________________ 3
Chapter 2. Exercises ___________________________ 5
Chapter 2.1 First Exercise: Basics ______________________ 5
Chapter 2.1.1 Step 1: Starting DAx ______________________________ 5
Chapter 2.1.2 Step 2: Loading a Data Set _________________________ 6
Chapter 2.1.2.1 Data Tags ___________________________________ 8
Chapter 2.1.2.2 Data Tags: Moving Data ________________________ 9
Chapter 2.1.2.3 Which mouse button should I use? _______________ 10
Chapter 2.1.2.4 Data Tags: Other Uses ________________________ 10
Chapter 2.1.3 Step 3: Zooming In on the Graph ___________________ 11
Chapter 2.1.4 Step 4: Printing the Graph, or Exporting It_____________ 12
Chapter 2.1.5 Step 5: Analysing the Data Set _____________________ 13
Chapter 2.1.5.1 Manually Adjusting Peaks ______________________ 14
Chapter 2.1.6 Step 6: Displaying Peak Data ______________________ 15
Chapter 2.1.7 Step 7: Saving Your Work (End of first workout) ________ 16
Chapter 2.2 Second Exercise: Shortcuts and More ________ 17
Chapter 2.2.1 Step 1: Opening Several Data Files - and Analysing Them 17
Chapter 2.2.2 Step 2: Adjusting a Baseline _______________________ 19
Chapter 2.2.3 Step 3: Finding Peaks ____________________________ 22
Chapter 2.3 Third Exercise: Capillary Electrophoresis _____ 23
Chapter 2.3.1 Step 1: Calculating Apparent Mobilities _______________ 23
Chapter 2.3.2 Step 2: Calculating Effective Mobilities _______________ 25
Chapter 2.4 Fourth Exercise: Gel Permeation
Chromatography _____________________________________ 27
Chapter 2.4.1 Step 1: Analysing the Calibration Sample _____________ 27
Chapter 2.4.2 Step 2: Setting the Flow Rate ______________________ 28
Chapter 2.4.3 Step 3: Creating the Calibration ____________________ 29
Chapter 2.5 Fifth Exercise: DNA Base Pair Count
Determinations ______________________________________ 31
Chapter 2.5.1 Step 1: Analysing the Calibration Sample _____________ 32
Chapter 2.5.2 Step 3: Creating the Calibration ____________________ 33
Chapter 2.5.3 Step 4: Using the Calibration _______________________ 35
Chapter 2.6 Sixth Exercise: Qualifying & Quantifying Peaks 36
Chapter 2.6.1
Chapter 2.6.2
Chapter 2.6.3
Chapter 2.6.4
Step 1: Analysing the Sample with Known Components __ 37
Step 3: Creating the Identification Database ___________ 38
Step 4: Using the Identification Database _____________ 41
Step 5: Setting up a Comparison Sheet ______________ 42
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