Download miRNA Microarray System with miRNA Complete Labeling and Hyb Kit

Procedures
Step 2. Prepare the 10X Blocking Agent
CAUTION
2
The sample must be completely dried after labeling. The presence of DMSO can cause
drying to take up to two hours. Residual DMSO will adversely affect the hybridization
results.
Step 2. Prepare the 10X Blocking Agent
1 Add 125 µL of nuclease-free water to the vial containing lyophilized 10X GE
Blocking Agent supplied with the Agilent miRNA Complete Labeling and
Hyb Kit (p/n 5190-0456).
2 Mix by gently vortexing. If the pellet does not go into solution completely,
heat the mix for 4 to 5 minutes at 37°C.
3 Drive down any material adhering to the tube walls or cap by centrifuging
for 5 to 10 seconds at 17,000 x g.
NOTE
10X Blocking Agent can be prepared in advance and stored at -20°C for up to 2 months.
After thawing, repeat the vortexing and centrifugation procedures before use.
miRNA Microarray System with miRNA Complete Labeling and Hyb Kit Protocol
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