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17 Incubate the NLT plate at room temperature for 2 minutes.
18 Centrifuge the NLT plate to 280 xg for 1 minute.
19 Place the NLT plate on the magnetic stand for 2 minutes or until the liquid appears
clear.
20 Remove the adhesive seal from the NLT plate.
21 Transfer 20 µl of clear supernatant from each well of the NLT plate to the
corresponding well of the new HSP plate labeled NLA. Take care not to disturb the
beads.
NOTE
Illumina recommends using a 20 µl single channel or multichannel pipette
set to 10 µl to perform two consecutive transfers of 10 µl to reduce sample
loss by ensuring that all of the liquid is transferred without disturbing the
beads.
22 [Optional] Load 1 µl of the tagmentation reaction remaining in the NLT plate on an
Agilent Technologies 2100 Bioanalyzer using a Agilent High Sensitivity DNA chip.
Check the size of the sample, which should produce a broad distribution of DNA
fragments with a size range from approximately 150 bp–1 kb.
Figure 2 Example TruSight Rapid Capture Post-Tagmentation Library Distribution
TruSight Rapid Capture Sample Preparation Guide
13
Clean Up Tagmented DNA
16 Mix thoroughly as follows:
a Seal the NLT plate with a Microseal ‘B’ adhesive seal.
b Shake the NLT plate on a microplate shaker at 1800 rpm for 1 minute