Download Assay Tips - Abacus ALS

Immunoassay Procedure
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• Cover the plate with a plate sealer before shaking.
Tips for Reducing Variability
• Ensure proper sample collection.
• The plate shaker speed should be increased to
agitate the plate at the highest speed that does not
lead to splashing on the sealer.
• To avoid low bead counts, thaw, vortex and
centrifuge all samples for 5-10 minutes at a
minimum of 3,000 x g. Avoid or remove any fat
layers that may develop.
Immunoassay Procedure
• Before running an assay, check the instrument the
• Centrifuge samples after thawing or if they appear
turbid. This is especially recommended for plasma
samples.
night before.
• Is the instrument calibrated?
• Has it been maintained?
• Have fresh water prepared, calibrated and
accurate pipettes, multichannel pipettes and an
• Ensure the proper mixing of samples and controls.
orbital shaker or alternative.
• Confirm availability of a cold room or
• Use appropriate pipetting technique:
refrigerator with power access for the orbital
• Hold the pipette at the same angle each time.
shaker.
• Use pipettes calibrated for values in the middle
range (not extremes).
• When running samples in duplicate, a maximum of
38 samples can be run per kit.
Running Your Assay
• Warm reagents to room temperature (20–25 °C)
before mixing. For assays requiring overnight
incubation in a cold room, warm reagents to room
temperature on the second day as well.
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A
Standard 0
(Background)
1
Standard 4
QC-2
Control
B
Standard 0
(Background)
Standard 4
QC-2
Control
C
Standard 1
Standard 5
Sample 1
D
Standard 1
Standard 5
Sample 1
E
Standard 2
Standard 6
Sample 2
F
Standard 2
Standard 6
Sample 2
G
Standard 3
QC-1
Control
Etc.
H
Standard 3
QC-1
Control
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96-Well Plate Map. Sample map showing placement of standards, QCs, background and samples.
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