Download Assay Tips - Abacus ALS

Transcript
Introduction
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Deciding Which MILLIPLEX®
Assay is Best for Your Research
• Merck Millipore’s ELISAs and MILLIPLEX® assays for
the same analyte commonly use the same antibody
pairs and conditions.
• In Method Comparison tests, while the
or analyte:
• Search the website for the catalog number.
Links to some of the references using the
absolute values are not exactly the same, the
product can be found at the top of the
results do correlate. Hence when switching
Product Description page.
from one assay platform to another, a
correlation factor may be used when
comparing with past data. In most situations
the dynamic range and the sensitivity of the
assay may be better with the MILLIPLEX® kits.
• Please contact Technical Support for more
information on correlation factors.
• To locate protocols and technical documents for a
specific panel:
• Search the website for the catalog number.
• For a more complete list, contact Technical
Support.
• To determine cross-reactivity for other species for a
panel or analyte:
• See the Species Cross-reactivity Tables in
Appendix 1.
• For intracellular assay kits, we analytically
validate the assay with human cell/tissue
culture samples. However, we provide the
species homology for each analyte in a table
• The link to the protocol can be found using:
on the product detail page on our website. We
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The “Documents” tab.
have also compiled a list of this information
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The right-hand side of the product name
and catalog number.
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The top of the Product Description page.
• The easiest way to find a panel that contains the
analytes you want to measure would be to search
online:
• The MILLIPLEX® Analyte Kit Finder located on
the MILLIPLEX® home page.
• Search the latest edition of
the Analyte Quarterly:
www.merckmillipore.com/milliplex
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• To find publications citing a specific panel
on our website:
-Kits: Kit Species Cross Reactivity:
www.merckmillipore.com/kits_species
-MAPmate™ assays: MAPmate™ Species
Cross Reactivity & Buffer Table:
www.merckmillipore.com/mapmates_sp
-Search the latest edition of the
Analyte Quarterly:
www.merckmillipore.com/milliplex