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Clone-it™ Enzyme-free Lentivectors Contents I. A. B. C. D. E. F. G. Introduction and Background Purpose of this Manual Advantages of the Lentivector Expression System Features and Benefits of the Clone-it™ Lentivectors Mechanism of Clone-it™ Lentivectors List of Components Additional Required Materials Safety Guidelines 2 2 2 3 5 6 6 II. Protocols A. Clone-it™ cDNA/miRNA/Reporter Lentivectors B. Clone-it™ shRNA Lentivectors 8 11 III. Troubleshooting 12 IV. References 13 V. A. Appendix Map and Features of the Vectors 1) Clone-it™ cDNA Lentivectors 2) Clone-it™ miRNA Lentivectors 3) Clone-it™ shRNA Lentivectors 4) Clone-it™ Reporter Lentivectors 14 14 15 16 17 B. C. Related Products Technical Support 18 18 VI. Licensing and Warranty Statement 888-266-5066 (Toll Free) 650-968-2200 (outside US) 18 Page 1