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Olympus IX-Series Non-Hoffman Condensers Refer to Figure 3.10 for a visual reference. Flip open one of the swing arm filter holders from the illumination pillar. Snap the CRi CP/IF optic into position. Be sure that the CRi logo reads from topto-bottom, and that the mirror-side of the optic is facing towards the illumination source. Figure 3.10. Installing the CRi CP/IF optic onto an Olympus IX microscope. The top, shiny surface of the CRi optic should face towards the illumination source, such that the CRi logo reads from top-tobottom when the optic is in place. Zeiss Axiovert 200 Non-Hoffman Condensers Remove the circular polarizer and filter from the CRi housing by unscrewing the optic. Be sure to maintain the orientation of the optics, such that the circular polarizer is inserted top side-up, as an inverted circular polarizer will prevent the system from working properly. Snap the circular polarizer into the middle plastic filter holder (as shown in Figure 3.11). Proceed to then snap the interference filter into the top filter holder. The arrow on the filter housing should face downward, in the direction of the sample stage. It is recommended to use gloves or surround the optic with lens paper as you insert it into the filter holders to avoid getting fingerprints on the face of the optics. To clean the optic, reference the helpful hint on Page 18. Figure 3.11. Installing the CRi CP/IF optic onto a Zeiss Axiovert 200 microscope. The circular polarizer should be placed into the middle filter holder, and the interference filter into the top filter holder as shown. Be careful to insert optics face-up. An inverted optic will prevent the system from working properly. Warning: The CP/IF for Olympus systems may look identical to that for Zeiss systems, but the circular polarizer is not the same. They are not interchangeable. Leica DM-IRB Non-Hoffman Condensers Place the CRi CP/IF filter ring in the microscope swing-arm, which is located above the turret condenser as shown in Figure 3.12. Be sure that the CRi logo is face-up. Swing Arm Figure 3.12. Installing the CRi CP/IF optic onto a Leica DM-IRB microscope. Page – 24 CRi, 35-B Cabot Road Woburn, MA 01801 USA :: (Toll-Free US) 1-800-383-7924, (P) +1-781-935-9099, (F) +1-781-935-3388 :: [email protected] :: www.cri-inc.com