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Figure 4-5. Proper alignment of the pump segment portion of the saline delivery tubing in the left and right guide slots of the tubing guides Ensure pump segment is aligned in the center of guide slots (upside down “v”) where it enters and exits the pump head Spiking the Saline Bag 1. Hang a bag of sterile saline (0.9% NaCl) solution on the Aquamantys Cart I.V. pole or another I.V. support which is in close proximity to the Aquamantys Pump Generator. 2. Remove the protective cover over the spike of the drip chamber at the end of the device’s saline delivery tubing. 3. Using aseptic technique, spike the bag of sterile saline (0.9% NaCl) solution. 4. Squeeze the drip chamber once or twice to fill the drip chamber to a level of at least one-third full. This is shown below in Figure 4-6. Figure 4-6. Spiking the saline bag 4-6