Download ESPRIT user manual 4.4.0-2

Chapter 4
75
4.7 – ESPRIT menu.
menu.
The ESPRITmenu contains commands to control the hardware functions of
the instrument.
Figure
Fig ure 4 .11 – ESPRIT menu items.
items .
4.7.1 – Manual Control of the Autolab SPR.
SPR.
Opens the pump control window used for manual control of a measurement.
Sampler position
controls and shows the position of the needles.
Pump 1 and 2 are syringe pumps
Aspirate/Dispense section to aspirate or dispense sample in or
out of the cuvette, the sample can be positioned in a microtiter
plate or a vial which is held under the needles.
Valve options control a three-way valve: valve to needle connects
channel from pump to cuvette. Valve to buffer connects channel
from buffer flask to pump.
Flow control enables to select the speed of the pump in µl/s.
Corresponds to the aspirating, dispensing and mixing speed.
Mix section for mixing the solution in the cuvette. Fill in the mixing
volume in µl and check the mix box to start mixing. This option
should only be used when the needles are inside the cuvette and
the mix volume should not exceed the cuvette volume.
Synchronized Pumps (Channels 1 & 2)
This section is meant for synchronized mixing of pump1 and
pump2. Set volume and Flow, check the mix box and both pumps