Download Troubleshooting Guide

Introduction
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The E.Z.N.A. Mag-Binds Forensic DNA Kit allows rapid and reliable isolation of
high-quality genomic DNA from Forensic. The Kit allows single or multiple
simultaneous processing of samples in under 1 hours. There is no need for
phenol/chloroform extraction, and time-consuming steps such as CsCl gradient
ultracentrifugation, and precipitation with isopropanol or ethanol, are
eliminated. DNA purified using the E.Z.N.A.® Mag-Binds® Forensic DNA Kit is ready
for applications such as PCR, Southern blotting and restriction digestion.
10. Add 50-100ul Elution Buffer or water to elute DNA from the magnetic
particles.
11. Resuspend Mag-Bind® particles by vortexing. Incubate 5-10 minutes at room
temperature.
12. Centrifuge at 10,000 x g for 1 min and transfer the supernatant into a new
tube. Store Purified DNA at -20O C.
Overview
If using the E.Z.N.A.® Mag-Binds® Forensic DNA Kit for the first time, please read
this booklet in its entirety to become familiar with the procedures. Forensic
samples lysed in a specially formulated buffer. The binding conditions are
adjusted so that genomic DNA will selectively bind to the Mag-Binds® . Two rapid
wash steps remove trace contaminants such as residual polysaccharides, and
pure DNA is eluted in Elution Buffer. Purified DNA can be directly used in
downstream applications without the need for further purification.
Yield and quality of DNA
Determine the absorbance of an appropriate dilution (20- to 50-fold) of the
sample at 260 nm and then at 280 nm. The DNA concentration is calculated as
follows:
DNA concentration = Absorbance260 × 50 × (Dilution Factor) :g/ml
The ratio of (absorbance260)/(absorbance280) is an indication of nucleic acid
purity. A value greater than 1.8 indicates > 90% nucleic acid. Alternatively,
quantity (as well as quality) can sometimes best be determined by agarose
gel/ethidium bromide electrophoresis by comparison to DNA samples of known
concentrations.
Storage and Stability
Most components of the E.Z.N.A.® Mag-Binds® Forensic DNA Kit, except RNase A
and OB Protease are stable for at least 24 months from date of purchase when
stored at 22oC-25oC. Mag-Bind® Particles Solution B should be stored at 4° C for
long-term use. Store RNase A at -20°C. OB Protease store at 15-25°C. During
shipment or storage in cool ambient conditions, precipitates may form in Buffer
MBL. Dissolve such deposits by warming the solution at 37oC and gently shaking.
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