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Pathway Profiling System User Manual List of Figures Figure 1. General scheme for the Pathway Profiling procedure 5 Figure 2. An overview of the cis-acting enhancer element vector constructs in the Pathway Profiling Systems 6 Figure 3. Generalized map of the Pathway Profiling Vectors 18 Figure 4. Map and multiple cloning sites of pTAL-SEAP/Luc Vector 19 Figure 5. Map and multiple cloning sites of pTA-SEAP/Luc Vector 20 Figure 6. Map and multiple cloning sites of pSEAP2-Control Vector 21 List of Tables Table I. Signaling Pathways Represented in the Pathway Profiling Systems 5 Table II. Culture Plate Conversion 11 Table III. Pathway Profiling Vector Information 16 Protocol No. PT3286-1 www.clontech.com Version No. 032712 Clontech Laboratories, Inc. 3