Download LineGene K Instruction Manual

Line-gene K Fluorescence Quantitative PCR Detection System

Absolute Quantitative Analysis Module
The bias in dashed line box is fitted by the known concentration standard sample’s Ct value.
There are 4 parameters to token the contrast line on the right. Y axis intercept, slope: math
parameters of the straight line. Error, correlation coefficient: represent the error of this analysis
result.

“Calculate Concentration and Ct value” column can display every sample’s calculated
concentration and Ct value.

When several standard samples with same concentration are chosen, user can refer to the
average Ct value, standard difference of Ct value, average concentration and standard
difference of concentration.
7.7.1. Application of Standard Curve
The standard curve is a contrast line that is used to calculate the concentration of sample. If there is no
standard sample set during the PCR experiment, user can import the given standard curve to do analysis
and calculation.

Create a Standard Curve
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