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COPAS™ BIOSORT INSTRUMENT Automated Analysis, Sorting, and Dispensing of small model organisms, beads, and particles SERVICE MANUAL Rev. 1.00 Last Updated On: May 8, 2003 Table of Contents TABLE OF CONTENTS ................................................................................................................... 2 INTRODUCTION ............................................................................................................................ 3 WARRANTY ................................................................................................................................. 3 WARNINGS AND PRECAUTIONS .................................................................................................... 4 SHIPPING CHECKLIST FORM ......................................................................................................... 5 For Customers located in North America, Japan....................................................................................................5 For Customers located in Europe (except United Kingdom)..................................................................................6 For Customers located in United Kingdom ............................................................................................................7 REAGENTS USED .......................................................................................................................... 8 Sheath Reagent .......................................................................................................................................................8 Control Particles .....................................................................................................................................................8 Cleaning Reagent ...................................................................................................................................................8 Bleach ...............................................................................................................................................................8 Sterilization Solution ..............................................................................................................................................8 MATERIALS REQUIRED FOR SERVICE (NOT PROVIDED) ............................................................... 9 WORK AREA AND FACILITIES SPECIFICATIONS ............................................................................ 9 Work Area Requirements .......................................................................................................................................9 Environmental Requirements .................................................................................................................................9 Air Requirements ...................................................................................................................................................9 Electrical Requirements........................................................................................................................................10 UNPACKING THE COPAS INSTRUMENT ..................................................................................... 11 OPTICAL FIBER INSPECTION, INSTALLATION AND CLEANING .................................................... 11 Procedure .............................................................................................................................................................11 FINAL SET-UP / ADJUSTMENT PROCEDURES .............................................................................. 13 Set Up .............................................................................................................................................................13 Software Verification ...........................................................................................................................................14 Fluidics Check......................................................................................................................................................15 Laser Power Adjustment ......................................................................................................................................16 Waste Tray Alignment .........................................................................................................................................17 PMT Set up...........................................................................................................................................................18 COPAS INSTRUMENT TEST ....................................................................................................... 19 Instrument Set-Up ................................................................................................................................................19 Performance Set-Up .............................................................................................................................................20 Performance Verification .....................................................................................................................................22 Sorter Operation Verification ...............................................................................................................................24 Instrument Preparation .........................................................................................................................................25 Instrument Test Worksheets .................................................................................................................................26 DAILY / SHORT TERM COPAS INSTRUMENT MAINTENANCE .................................................... 27 Daily Maintenance Procedure ..............................................................................................................................27 Visual Tubing Check............................................................................................................................................28 ANNUAL PREVENTATIVE MAINTENANCE PROCEDURE ............................................................... 29 SYSTEM INTERCONNECT DRAWINGS .......................................................................................... 31 GENERAL PARTS LIST ................................................................................................................ 32 PRINTED CIRCUIT BOARDS ........................................................................................................ 33 MAJOR CABLE ASSEMBLIES....................................................................................................... 33 CONTACT INFORMATION ............................................................................................................ 34 NOTES ........................................................................................................................................ 34 Union Biometrica, Inc. Confidential www.unionbio.com 2 Introduction The COPAS BIOSORT automates the analysis, sorting, and dispensing of objects ranging from 40 - 125 microns including particles, beads, seeds, and small model organisms. Using the physical parameters of object length, optical density, and the intensity of fluorescent markers, objects are sorted according to user selectable criteria, and then may be dispensed into stationary bulk receptacles or multi-well microtiter plates. This COPAS BIOSORT Service Manual details those procedures pertinent to the maintenance of the COPAS instrument and all top level assemblies that are used in the manufacture of the instrument. Requests for more detailed lower level parts and materials will be addressed by Union Biometrica, Inc. on an individual basis. Warranty The Union Biometrica COPAS instrument is warranted to be free from defects in material and workmanship for a period of one (1) year from the date of installation and acceptance. Union Biometrica or authorized personnel will repair, at our election, by replacement, any part of the instrument, which fails within the warranty period. As a part of this warranty period, the customer will receive unlimited telephone assistance support Monday through Friday, during normal working hours from 9:00 AM-5:00 PM Eastern Standard Time, up to four (4) emergency on-site visits per year, all replacement parts, labor, and travel, and one (1) preventive maintenance visit per year. This warranty is extended to the original buyer purchasing the equipment from Union Biometrica or authorized distributor. This warranty does not include routine cleaning, adjustment or normal wear and tear from use unless designated by buyer as its annual preventative maintenance visit and excludes: (A) Service or parts required due to damage caused by accident, neglect, breakage, misuse, alteration to the equipment, electrical current fluctuations, unfavorable environmental conditions, work performed by an unauthorized agent, or forces of nature; (B) work which, in the sole exclusive opinion of Union Biometrica, is impractical to perform because of the platform’s location or proximity to any other device; (C) modifications to the instrument for performance outside of the published performance specifications; (D) Service required to parts in the system that have been exposed to or otherwise affected by solutions or reagents not manufactured by Union Biometrica , or another authorized manufacturer, which causes poor or erratic performance and / or shortened component life; (E) Service performed by an unauthorized third party; or (F) instrument refurbishing for cosmetic purposes. All parts replaced under the original warranty will be warranted only until the end of the original instrument warranty. Union Biometrica must receive all requests for warranty replacement or authorized distributor within thirty (30) days after the component failure. It is the owner's responsibility to maintain the instrument in accordance to Union Biometrica's guidelines. Union Biometrica reserves the right to change, alter, modify or improve any of its instruments without obligation to make corresponding changes to any instrument previously sold OR shipped. All service will be rendered during Union Biometrica's normal hours of operation. Repairs requested outside of normal business hours will be performed at Union Biometrica's discretion, at prevailing after hour rates. Such requests must be accompanied by an approved purchase order. Union Biometrica, Inc. Confidential www.unionbio.com 3 The following exceptions apply: 1. Consumable items, including reagents and solutions, pump tubing, tubing, are warranted to be free of defects at the time of installation. Union Biometrica does not warrant parts and accessories not manufactured by or for Union Biometrica. Union Biometrica will assist buyer in obtaining any information or service from any manufacturer for any part not covered by Union Biometrica. 2. Freight charges are at the customers’ expense. The warranty is invalid if: 1. The COPAS platform is used for purposes outside the scope of supported applications and specifications. 2. System modifications, not approved by Union Biometrica in writing, are performed. Warnings and Precautions The COPAS BIOSORT is a Class I laser product containing a Class IIIA laser. The COPAS Argon Laser System contains a Class III B laser, which is fiber coupled through a fiber optic. Only qualified service personnel should remove the COPAS Argon Laser System or the optics assembly covers. The COPAS BIOSORT is comprised of the Dispenser and the Argon Laser System. This term is used in reference to the combination of these two components. Use of the COPAS BIOSORT Dispenser and Argon Laser System in a manner not specified by Union Biometrica may impair the protection provided by the equipment. The COPAS instrument will be factory shipped to you in protective packaging and will be installed by a trained Union Biometrica, Inc. representative. The Union Biometrica service representative will also verify the system performance. If the instrument must be moved or maintenance must be performed, Union Biometrica, Inc. recommends that a trained Union Biometrica representative install the system and perform most maintenance functions. The connection of the Laser fiber optic cable requires special attention and should not be attempted by untrained persons. In order to prevent the system from overheating, ensure that the installation permits the unrestricted flow of air around the system components and confirm prior to installation that the Work Area and Facilities specifications have been met. The COPAS BIOSORT should be used only by trained laboratory personnel. Use of this instrument by an untrained operator could result in damage to the product or injury to the operator. Other than procedures outlined in the Operator’s Manual, there are no other operator required electrical, optical, or mechanical adjustment or serviceable components. Please refer to the Operator’s Manual for complete Safety Warnings and Precautions. Union Biometrica, Inc. Confidential www.unionbio.com 4 Shipping Checklist Form For Customers located in North America, Japan COPAS BIOSORT Part Number: Quantity 1 1 1 1 1 1 2 20 ft. 1 1 1 1 1 2 1 1 1 1 1 1 1 350-5000-000 Serial Number:_______________________ Description Power Cord Cable Power Strip RS-232 Data Cable CSM Cable - ReFLX ONLY Laser Power Cord Strait Blade Laser Plug Tubing Clamp PVC Air Tubing Threaded/Barbed Air Fitting Slotted Screwdriver Waste Bottle Vent Tubing Waste Pump Tubing GP Sheath Reagent and MSDS Cleaning Reagent and MSDS Installation Disc Laser System Cable GP Control Particles and MSDS Bubble Trap Filter (only with ReFLx) Reference Manual Information Packet Part Number 066-0004-000 066-0007-500 066-0009-010 066-0009-020 067-0020-004 067-0030-001 100-0010-001 111-0250-002 119-0003-000 145-0020-000 300-5044-000 300-5062-000 300-5068-000 300-5070-000 300-5072-000 300-5080-____ 310-5018-000 310-5071-000 340-5014-000 350-5074-000 Packaged by: ___________________ Date: ______Inspected by: ________________Date: ______ Union Biometrica, Inc. Confidential www.unionbio.com 5 For Customers located in Europe (except United Kingdom) COPAS BIOSORT Part Number: Quantity 1 4 1 1 1 1 1 2 20 ft. 1 1 1 1 1 2 1 1 1 1 1 1 1 350-5000-000 Serial Number:_______________________ Description Power Strip Jumper Cables Power Strip/Wall Cable Laser/Wall Cable Compressor/Wall Cable RS-232 Data Cable CAM Cable - ReFLX ONLY Tubing Clamp PVC Air Tubing Threaded/Barbed Air Fitting Slotted Screwdriver Waste Bottle Vent Tubing Waste Pump Tubing GP Sheath Reagent and MSDS Cleaning Reagent and MSDS Installation Disc Laser System Cable GP Control Particles and MSDS Bubble Trap Filter (only with ReFLx) Reference Manual Information Packet Part Number 066-0007-000 066-0007-100 066-0007-200 066-0007-300 066-0007-400 066-0009-010 066-0009-020 1 0 0 -0 0 1 0 -0 0 1 111-0250-002 119-0003-000 145-0020-000 300-5044-000 300-5062-000 300-5068-000 300-5070-000 300-5072-000 300-5080-____ 310-5018-000 310-5071-000 340-5014-000 350-5074-000 Packaged by: ___________________ Date: ______Inspected by: ________________Date: ______ Union Biometrica, Inc. Confidential www.unionbio.com 6 For Customers located in United Kingdom COPAS BIOSORT Part Number: Quantity 1 4 1 1 1 1 1 2 20 ft. 1 1 1 1 1 2 1 1 1 1 10 1 1 350-5000-000 Serial Number:_______________________ Description Power Strip Jumper Cables Power Strip/Laser/Wall Cable Laser/Compressor/Wall Cable RS-232 Data Cable CSM Cable - ReFLX ONLY Laser Power Cord Tubing Clamp PVC Air Tubing Threaded/Barbed Air Fitting Slotted Screwdriver Waste Bottle Vent Tubing Waste Pump Tubing GP Sheath Reagent and MSDS Cleaning Reagent and MSDS Installation Disc Laser System Cable GP Control Particles and MSDS Bubble Trap Filter (only with ReFLx) Reference Manual Information Packet Part Number 066-0007-000 066-0007-100 066-0007-310 066-0007-410 066-0009-010 066-0009-020 067-0020-003 1 0 0 -0 0 1 0 -0 0 1 111-0250-002 119-0003-000 145-0020-000 300-5044-000 300-5062-000 300-5068-000 300-5070-000 300-5072-000 300-5080-____ 310-5018-000 310-5071-000 340-6000-000 350-5074-000 Packaged by: ___________________ Date: ______Inspected by: ________________Date: ______ Union Biometrica, Inc. Confidential www.unionbio.com 7 Reagents Used Do not use unapproved solutions in the COPAS BIOSORT. The use of unapproved solutions can cause damage to the device and will void the warranty. The specialized reagents discussed below are available from Union Biometrica, Inc. Material Safety Data Sheets are enclosed in every shipment of Union Biometrica, Inc. supplied reagents and are also available upon request. NOTE: Performance Specifications are only valid with Union Biometrica, Inc. reagents. Sheath Reagent Sheath reagent is an aqueous based reagent containing surfactant. The sheath reagent must be compatible with the control particles used and the sample to be analyzed. For most applications, COPAS GP Sheath, PN 300-5070-000 is recommended. Two 20 Liter containers of PN 300-5070-000 are shipped with each COPAS BIOSORT instrument. Control Particles Control particles are latex beads that are uniform in size, suspended in a reagent compatible with the sheath reagent and sample to be analyzed. For most applications, GP 42 micron HF Control Particles, PN 310-5071-000 are recommended. One 1 Liter container of PN 310-5071-000 is shipped with each COPAS BIOSORT instrument. Cleaning Reagent Cleaning reagent is an aqueous based reagent containing surfactant specially formulated for use with all COPAS platforms. This solution should be used for daily cleaning procedures Cleaning reagent should be used for daily maintenance. One 1 Liter container of PN 300-5072-000 is shipped with each COPAS BIOSORT instrument. Bleach Bleach is used on the COPAS instrument for troubleshooting procedures including breaking up proteins potentially causing a blockage in the flow cell or fluidics. Bleach is also used to clean the internal flow cell. The manufacturer does not supply ethanol. NOTE: DO NOT use on external surfaces of optical components UNION BIOMETRICA, INC. recommends that Clorox® brand bleach be used due to its observed low particulate count. If a different brand of bleach is used, filtering is required. When using bleach, use 5% sodium/calcium hypochlorite at a 50% dilution. Sterilization Solution 70% Ethanol is used on the COPAS instrument for sterilization procedures and in certain troubleshooting procedures. The manufacturer does not supply ethanol. Union Biometrica, Inc. Confidential www.unionbio.com 8 Materials Required for Service (Not Provided) • • • • • • • Screwdrivers: 10”+ length and jewelers size Oscilloscope & PMT wires Needle nose pliers Paper towels Cable ties to secure tubing and cables 50 mL syringes for removing liquid from the sample cup 96 well plates and lids • Argon Laser Alignment Glasses [2-3@488-515nm, amber lens, 70% VLT] – Only required if service will be performed on the Argon Laser by the customer. Union Biometrica highly recommends that any laser service be performed by a Union Biometrica trained service person. Work Area and Facilities Specifications Prior to installation of the COPAS BIOSORT instrument, confirm that the work area and facilities comply with the below specifications. Work Area Requirements • • • The COPAS system requires a permanent, open, level, vibration free, working space with the dimensions of 3 feet (1 meter) deep x 7 feet (2.3 meters) wide x 4.75 feet (1.4 meters) high. No other equipment should be placed over the COPAS system. There also should be user access on both sides of the working area. A COPAS system with the optional Zymark Twister® Robot will need an additional space to the left of the COPAS of 3 feet (1 meter) deep x 2 feet (0.6 meters) wide x 2 feet (0.6 meters) high. The Union Biometrica supplied optional Air Compressor requires approximately 14 inches (36 centimeters) deep x 18 inches (46 centimeters) wide x 20 inches (51 centimeters) high of stable floor space. Environmental Requirements • • • • This instrument is designed for use at an altitude of up to 2000 meters, in an ambient operating temperature between 15° and 30°C (60° to 85°F) with a relative humidity of 0% to 85% noncondensing, decreasingly linearly to 50% relative humidity at 40°C (104°F). Temperature should not fluctuate more than +/- 1.5 °C from the time of experimental setup through completion or adjustments will need to be made. Ensure that adequate ventilation of the system components is provided. It is important that ventilation openings not be blocked while the system is powered ON. The system generates approximately 3800 BTU/Hr. This instrument is rated, per IEC 60529, for installation in an IP00 environment. It is intended that it be installed in a laboratory environment protected from dust and spray. Air Requirements • The input air pressure should be at least 50 psi but no more than 100 psi of filtered, at 2 CFM, of non-condensing, water and oil free air. The optional COPAS Air Compressor is available for laboratories that cannot meet these requirements. Union Biometrica, Inc. Confidential www.unionbio.com 9 Electrical Requirements • • • • • Place the COPAS system and components within 6 feet (2 meters) to the power outlets. COPAS systems and the Laser should each be connected to separate, dedicated electrical supply circuits, using the supplied detachable line cords (or CE7/VII approved equivalent detachable cords for Europe). The supply circuits must be compliant with local electrical codes, and must include a dedicated protective ground connection to protect the system. The main supply voltage is not to exceed 10% of the nominal voltage. Place the instrument so that the detachable line cords remain readily accessible. This is essential to permit use of the line cords as the emergency disconnection devices in case of a fault. Circuit Requirements for 120 VAC Countries: o COPAS Instrument, PC & monitor – 100-120VAC, 15 Amp, 50/60 HZ, single phase on a dedicated line with protective earth ground. o Argon Laser System – 100-120VAC, 20 Amp, 50/60 HZ, single phase on a dedicated line with a protective earth ground. o Air Compressor (optional) – 100-120VAC, 13 Amp, 50/60 HZ, single phase on a dedicated line with protective earth ground. (May be placed on instrument line if necessary if surge protection is provided for the instrument, PC, and monitor.) Circuit Requirements for 230 VAC Countries: o COPAS Instrument, PC & monitor – 220/240VAC, 10 Amp, 50/60 HZ, on separate dedicated lines with protective earth ground. o Laser – 220/240VAC, 16 Amp, 50/60 HZ, on separate dedicated lines with protective earth ground. o Air Compressor (optional) – 220/240VAC, 10 Amp, 50/60 HZ, single phase on a dedicated line with protective earth ground. (May be placed on instrument line if necessary if surge protection is provided for the instrument, PC, and monitor.) Union Biometrica, Inc. Confidential www.unionbio.com 10 Unpacking the COPAS Instrument General recommended guidelines for unpacking a COPAS instrument and laser assembly. 1. Confirm work area and facilities meet specifications outlined by Union Biometrica in this Service Manual. 2. Inspect all containers for damage. If there is any damage, contact Union Biometrica immediately to report the damage. 3. Inventory all containers. It is recommended that, if storage area is available, customers keep the COPAS instrument containers to be used for long term instrument storage, or moving the instrument from one location to another. 4. Remove system components from containers and move to laboratory bench where the system will be permanently located. 5. Due to the weight of the instrument [40 Kg (88 lbs)], a minimum of two people is required to move the COPAS BIOSORT. In order to lift the unit safely without tipping, one person should be located on either side of the instrument to lift the unit by placing one hand under the front and one under the rear of the base. 6. Due to the weight of the external laser, [33.6 Kg (74 lbs)], a minimum of two people is also required to move the unit. Lift the Argon Laser System by placing one hand under the front and one under the rear of the base. 7. Connect all cables, tubing, and bottles according to COPAS BIOSORT System Interconnect Drawing and COPAS BIOSORT Fluidic Diagram contained in this Service Manual. Optical Fiber Inspection, Installation and Cleaning According to UBI Operational Instruction: OI 112, Optical Fiber Inspection, Installation and Cleaning Instructions, Revision No. 1, (Last revision date: 1/22/02) Purpose: The purpose of this OI is to describe the pre-installation inspection procedure, the installation procedure, the installation inspection procedure and the cleaning procedure of an Optical Fiber. NOTE: Use proper laser safety precautions at all times while performing these steps. Always wear argon safety glasses. Procedure 1. Pre-Installation Inspection a. Set the laser to STAND-BY mode. b. Close laser shutter. c. Remove the fiber from the instrument. d. Position the fiber approximately 12” (30cm) from a diffuse white target. e. Open the laser shutter. f. Examine the beam pattern for a uniform circular beam. Union Biometrica, Inc. Confidential www.unionbio.com 11 g. If a uniform circular beam is observed, proceed to Installation, Step 2.2. If concentric rings, multiple patterns or any other distortions are observed, go to Cleaning Procedure, Step 2.4 and then repeat Steps 2.1.1 through 2.1.6 one time. h. If concentric rings, multiple patterns or any other distortions are still observed, the fiber is damaged and a new fiber must be inspected. 2. Installation a. Set the laser to STAND-BY mode. b. Close laser shutter. c. Soak a cotton swab with 100% Methanol. d. Clean the fiber receptacle with the soaked cotton swab. e. Insert the fiber into the receptacle in the optical assembly ensuring the fiber face does not come in contact with the flat surface in between the threaded ring and the receptacle bore. 3. Installation Inspection a. Open the laser shutter. b. Place a beam-blocking device into the beam path between the spherical lens and the cylindrical lens mount. c. If a uniform circular beam is observed, installation is complete. If concentric rings, multiple patterns or any other distortions are observed, go to Cleaning Procedure, Step 2.4 and then repeat Steps 2.3.1 through 2.3.2 one time. d. If concentric rings, multiple patterns or any other distortions are still observed, the fiber is damaged and a new fiber must be installed. 4. Cleaning Procedure a. Set the laser to STAND-BY mode. b. Close laser shutter. c. Soak a piece of lens tissues with 100% methanol. d. Clean the fiber face with soaked lens tissue. e. Dry the fiber face with a second piece of lens tissue. f. Open the laser shutter. g. Perform appropriate Inspection (pre-installation or installation). h. If a uniform circular beam is observed, return to appropriate step. If concentric rings, multiple patterns or any other distortions are still observed, perform steps 2.4.1 through 2.4.5 one time. i. If concentric rings, multiple patterns or any other distortions are still observed, the fiber is damaged and a new fiber must be inspected. Union Biometrica, Inc. Confidential www.unionbio.com 12 Final Set-Up / Adjustment Procedures According to UBI Operational Instruction: OI 109, Final Set-Up / Adjustment Procedures Instructions, Revision No. 3, (Last revision date: 11/09/01) Purpose: The purpose of this OI is to list and describe the steps required to prepare a system for Final Test. Before each COPAS BIOSORT instrument is delivered to a customer, the system is tested according to the following procedures and should never need to be performed on site by a customer. Some references to internal Union Biometrica documents are made, for a copy of the referenced document, please contact the factory. Set Up 1. Instrument a. Attach all cables and visually inspect system. b. Turn ON system c. Set power supply DC voltage output for 5.10vdc on sorter board as follows: d. Using Out1 voltadj, place meter on power connect J12 (Black to Black and Red to Orange). e. Adjust meter reading to 5.11. f. Check cap #C171 located below Serial Number Prom. Meter must read 5.10. g. Set the mixer stepper motor drive current on the bulkhead board as follows: h. Place a meter across C5 on bulkhead board. i. Adjust DC voltage output using POT1 for a meter reading of 0.250v ± .005v. j. 635nm Laser: k. Verify switch #3 from bottom on the Laser Dipswitch SW1 (located on the bulkhead board) is ON (to the left). l. 670nm Laser: m. Verify switch #1, #2, #3 from bottom on the Laser Dipswitch SW1 (located on the bulkhead board) is ON (to the left). n. Set the dipswitches on the waste pump stepper motor to the following: BIOSORT SELECT, PLUS, XL 24/36v ON ON 1P/2P ON ON F/H OFF ON ACD ON ON Set the dipswitches on the two stage drivers to the following: BIOSORT, SELECT, PLUS, XL 24/36v ON 1P/2P ON F/H OFF ACD ON o. Clean all bottles. Fill sheath bottle and backwash bottle with Sheath Reagent. p. Check the sample cup position. Union Biometrica, Inc. Confidential www.unionbio.com 13 2. Computer a. Turn ON computer and install PCI BUS Ether Netcard per OI 110. b. Install COPAS System Software using Installation Disk. c. Install Source Disk and open Key Maker. d. Enter System ID number and options. The system will issue a new registration code. e. Highlight and Ctrl+C the new registration code. f. Open the system software. Open About MC menu and select Registration. g. Place the cursor in the registration code box and hit Ctrl+V. Ensure the new registration code has been inserted. Verify the System ID number matches the number in Key Maker. h. Right click on the COPAS icon. Select Property, select Find Target, and Select mc.ini. i. Verify that the correct values are set in the ini.file according to the attached examples. Open the file menu and select save. j. Perform the following set up on computer: k. Initialize Network Port Enable l. Select System Printer m. Install WINMDI, POWERWARE, Registration Code and Communications Port Software from UnioBio system folder. 3. Argon Laser a. Plug Argon Laser into correct voltage. b. Move yellow wire on panel meter to top right-hand position (2 decimal points) of connector. c. Turn ON AC power. d. Verify 2 decimal points on LCD. e. Power up the computer and open the program by double clicking on the COPAS Icon. Open TOOLS Menu and select “Run External Laser”. Turn laser on in Run Mode. f. Set laser power to 10 ± 2 mW on the laser power supply by placing a laser meter, set to 488nm, between the laser and cylindrical lens and adjusting the Int Adj located in the bottom of the Argon laser. g. Set the Argon laser readout to within ± 1mW of the laser power recorded in step 3-E by adjusting POT 3, DVM ADJ. h. Set main screen laser power to within ± 1mW of the laser power recorded in step 3-E by adjusting POT 1, MON GAIN. i. On the main computer screen, open TOOLS Menu and select “Run External Laser”. Change laser to the Standby Mode. j. With the laser power meter still in place, record the laser power. k. Compare the Argon laser readout and the main screen laser power to the laser power meter. l. Adjust the three readings to be as close as possible. m. Set the laser back into Run Mode and verify the three readings in Run Mode still meet the ± 1mW specification. If not, readjust to be within specification and repeat steps 3-I through 3M. Software Verification 1. With computer powered up, verify the following: a. WINMDI software present b. Powerware software present c. Registration Number corresponds with System Type and Serial Number. d. INI.file values set according to attached samples. Union Biometrica, Inc. Confidential www.unionbio.com 14 Fluidics Check 1. Measure the length of the waste pump tubing. 2. Connect and turn ON External Waste Pump, if applicable. 3. Add approximately 20ml of filtered bleach to Sample Cup. 4. Select START and allow the system to pressurize. Adjust the pressures to the following based on the size of the flow cell: 250µ Sheath Sample Sorter Cleaning 4.0 – 5.0 5.0 – 7.0 3.0 – 4.0 10.0 – 11.0 500µ Sheath Sample Sorter Cleaning 2.5 – 3.5 1.2 - 1.8 3.0 – 4.0 10.0 – 11.0 1000µ Sheath Sample Sorter Cleaning 4.0 – 5.0 2.0 – 3.0 3.0 - 4.0 10.0 – 11.0 5. Select Pressure OK. 6. Select Sample Valve and run until cup is empty. 7. Rinse Sample Cup with distilled water three times. 8. Fill sample Cup with distilled water and run until cup is empty. 9. Refill Sample Cup with distilled water. 10. Turn Sheath Valve ON and check for flow. 11. Turn Sample Valve ON and check that the valve opens and closes. Ensure that there are no leaks. 12. Turn Sample Valve OFF. 13. Select Clean and verify flow increases through sheath tubing. 14. Turn Sheath Valve OFF. 15. Verify mixers are spinning. 16. Turn Sheath Valve ON and Measure the flow rate. Adjust the sheath pressure to obtain a flow rate as follows: 250µ 9-10 ml/min Union Biometrica, Inc. Confidential www.unionbio.com 500µ 20-25 ml/min 1000µ 30-35 ml/min 15 Laser Power Adjustment 1. Using a Laser Power Meter set to 635nm, place the power paddle between the laser and the cylindrical lens until the maximum meter reading is located. 2. Adjust the screw on the POT located on the laser supply board to obtain EXT voltages as follows: 250µ 2.5 – 3.0 volts 500µ 1.0 – 1.5 volts 1000µ 2.5 – 3.0 volts within an approximate laser power range of: 250µ 0.75 – 1.25 mW 500µ 1.5 – 2.5 mW 1000µ 0.5 - 1.0 mW Optical Fine Tuning NOTE: Use proper laser safety precautions at all times while performing these steps. 1. Connect channel 1 on the oscilloscope to the EXT Test Point on the Pre-Amplifier. 2. Connect channel 2 to the Green PMT. 3. Set up the oscilloscope using a 1X probe with settings of 1.00 v on both channels and 25.0 ms. 4. Fill Sample Cup with appropriate beads and Turn Sheath and Sample Valves ON. 5. Turn the flow cell adjuster screw on the optical assembly to obtain maximum peak height on the oscilloscope. 6. Move the Cylinder Lens toward the flow cell to obtain the narrowest peak (~ 25µsec wide) with rounded bell-shaped curve. Do not allow the top of the peak to flatten. 7. Remove the EXT detector and place the mask onto the optical assembly. 8. Align the sensor mask by centering the red line on the laser beam. 9. Place the rod holder, spacer and EXT detector onto the optical assembly. 10. Print a copy of the ini.file and place into the DHR. Diverter Alignment 1. Position the Sorter Valve ~ 1 stream diameter away from the flow stream. 2. Turn sheath valve and sorter valve ON. 3. Looking directly at the spray of sheath, adjust both the front and back diverter adjustment screws until the spray is centered. 4. Adjust the Sorter pressure to obtain a stream deflection of ~ 30° from vertical. Union Biometrica, Inc. Confidential www.unionbio.com 16 Waste Tray Alignment 1. Turn the waste tray adjuster screw counterclockwise and push the tray toward the right as far as it will go. 2. Turn the sheath valve ON and Sorter Valve OFF. 3. Turn the screw clockwise until a stream of sheath just flows through the opening. Ensure the stream is flowing straight. 4. Turn the Sorter Valve ON and check that no dripping occurs. 5. Verify that the stream hits the waste tray just after the bend in the metal plate. Stage Alignment 1. Open the TOOLS menu and select Align Stage. 2. Place a 96 well plate onto the stage. 3. Click the WELL A1 button and then click the TEST DROP button to verify that the test drop has been deposited neatly into the correct well. 4. Adjust the alignment by changing the <OUT IN> and <LEFT RIGHT> positions by increasing or decreasing amount of steps if the stage positioning is incorrect. This may be done using the arrow buttons next to each of these fields. Repeat the TEST DROP procedure until the drop falls in the center of the well. 5. Click the NEXT COL or NEXT ROW buttons to allow for additional positions to be checked. Continue through at least ½ of the first row of the 96 well plate. 6. Click the LAST WELL button and then click the TEST DROP button again to verify correct stage alignment. 7. Return to WELL AT A1 and verify drop is still in center of well. 8. Repeat the above alignment procedure using LOAD B and WELL B1 for aligning the B side of stage if necessary. 9. Observe the droplet formation and verify there is no more than 1 satellite drop. If a satellite drop is present, it must fall within the well area. If there is more than 1 satellite drop, troubleshoot and repeat DROP TEST. Click the DONE button, which will return the view to the COPAS software main screen. 10. Save the stage alignment upon exiting the program. The alignment will be saved in the COPAS system software. Union Biometrica, Inc. Confidential www.unionbio.com 17 PMT Set up NOTE: Move the oscilloscope lead from PMT to PMT as appropriate to obtain necessary data. 1. Set Green and Red PMTs to 500 on computer main screen. Record voltage outputs from channel 2 on the oscilloscope. 2. Open TOOLS Menu and select SELECT PMT FILTERS. Change to Filter Set 2. 3. Set Yellow PMT to 500 on computer main screen. Record the voltage output from channel 2 on the oscilloscope. 4. Replace the 488nm filter with the 514nm filter. 5. Open TOOLS Menu and select SELECT PMT FILTERS. Change to Filter Set 3. 6. Ensure the Red and Yellow PMTs are set at 500 on the computer main screen. Record voltage outputs from channel 2 on the oscilloscope. 7. Replace the 514nm filter with the 488nm filter. 8. Adjust red PMT on computer main screen to obtain a voltage output of 1.5 volts on the oscilloscope. 9. Adjust Yellow PMT on computer main screen to obtain a voltage output of 1.5 volts on the oscilloscope. 10. Open TOOLS Menu and select SELECT PMT FILTERS. Change to Filter Set 1. 11. Adjust Green PMT on the computer main screen to obtain a voltage output of 1.5 volts on the oscilloscope. Union Biometrica, Inc. Confidential www.unionbio.com 18 COPAS Instrument Test According to UBI Procedure: 350-5000-TPR, COPAS BIOSORT Test Procedure, Revision No. 2 (Last revision date: 9/26/01) Instrument Set-Up 1. Ensure air supply is connected to the instrument and turned ON. Set pressure to 20-25 psi. 2. Turn ON instrument main power and laser power supply. 3. Turn ON computer and record the following: E-Prom Number: Instrument Serial Number: Registration Number: Software Rev: Firmware Rev: 4. Measure laser power. Start the instrument and document the laser power from the main screen and the laser power supply. Specification Blue Laser Power: Main Screen Laser Power: Power Supply Laser Power: Red Laser Power: ________mW ________mW ________mW ________mW 10 ± 1.0 mW ± 1.0 mW from Blue Laser ± 1.0 mW from Blue Laser 0.8 – 1.05 mW 5. Set up pressures. Place approximately 4 L of BIOSORT Sheath Reagent (P/N 350-5071-000) into the sheath container and cap firmly. a. Place approximately 4 L of BIOSORT Sheath Reagent (P/N 350-5071-000) into the sheath container and cap firmly. b. Place approximately 200mL of distilled water into the secondary sample container and cap firmly. c. Place approximately 500mL of BIOSORT Sheath Reagent (P/N 350-5071-000) into the cleanout bottle. d. Select START on the main screen and wait approximately 1 minute for pressures to equilibrate. e. Verify both stir bars are spinning. If not, turn mixer OFF, re-position stir bars and turn mixer back ON. If stir bars are still not mixing, troubleshoot instrument. f. Make sure that all lines are air free. g. Turn SORTER Pressure OFF. Measure the flow rate of the Sheath. The flow rate of the sheath must be 8 – 10 ml/min. Adjust pressure to obtain this flow. Flow Rate: __________ Final Pressure: __________ h. Adjust sample pressure to obtain minimal counts/sec. Adjust the following, if necessary, and record final pressures. Union Biometrica, Inc. Confidential www.unionbio.com 19 NOTE: Sample pressure must be at least 1.0 psi greater than sheath pressure. Settings Actual Sheath 3.5 – 5.0 psi __________ Sample 5.0 – 7.0 psi __________ Sorter 3.0 – 4.0 psi __________ Clean 9.0 – 11.0 psi __________ Performance Set-Up 1. Select PRESSURE OK. 2. Open the FILE Menu and select the 96serp file. 3. Clean the Sample Cup, Sample Valve and Flow Cell. According to UBI Procedure: OI 05, Sample Cup, Sample Valve and Flow Cell Cleaning, Revision No. A (Last revision date: 9/29/00) a. Click OFF the SAMPLE VALVE checkbox. b. Detach the sample tubing and remove cap from primary sample cup. Discard any remaining sample. c. Fill primary sample cup with 40 mL of deionized/distilled water and firmly tighten the cap. Reattach sample tubing to the primary sample cup. d. Re-check the SAMPLE VALVE checkbox. e. Process the water until the sample cup is empty. f. Click OFF the SAMPLE VALVE and SHEATH VALVE checkboxes. g. Detach the sample tubing and remove cap from primary sample cup. h. Fill primary sample cup with approximately Bleach solution, refer to Section 8.5.5 of the COPAS BIOSORT Operator’s Manual for bleach concentration specifications, replace the cap and firmly tighten. Reattach sample tubing to the primary sample cup. i. Select the Acquire button and process the bleach for approximately 10 minutes. j. Click OFF the SAMPLE and SHEATH VALVE checkboxes. k. Detach the sample tubing and remove cap from primary sample cup. l. Fill the primary sample cup with 40 mL of deionized/distilled water. m. Aspirate deionized/distilled water and discard. n. Fill primary sample cup again with 40 mL of deionized/distilled water and firmly tighten the cap. Re-attach sample tubing to the primary sample cup. o. Select the Acquire button and process the deionized/distilled water for approximately 10 minutes. Do not allow the sample cup to run dry. p. Click STOP twice. The system is now cleaned. q. Clean external surfaces of any spillage using a water dampened lint free cloth. 4. Place approximately 20 mL of BIOSORT CONTROL BEADS (P/N 310-5071-000) into the primary sample cup and cap firmly. 5. Set PMTs as follows: PMT Control Green 500 Yellow 500 Red 500 6. Connect oscilloscope to the Green PMT and adjust the voltage to 1.5 volts using the PMT Control. Union Biometrica, Inc. Confidential www.unionbio.com 20 7. Connect the oscilloscope to the Red PMT and adjust the voltage to 1.5 volts using the PMT Control. 8. Open the TOOLS Menu and select “SELECT PMT FILTERS”. Choose Filter Set 2, Green and Yellow, and restart the external laser. 9. Connect the oscilloscope to the Yellow PMT and adjust the voltage to 1.5 volts using the PMT Control. 10. Record the final PMT values. PMT Control Green Yellow Red 11. Open TOOLS menu and select RUN CONTROL PARTICLES. 12. Adjust sample pressure to obtain 10 ± 2 beads/sec by selecting ACQUIRE to begin particle flow. Collect beads for 1 minute and calculate the mean rate of the beads. Total Mean Rate (Total /60) Sample Pressure: ____________ Specification ____________ 10 ± 2 / sec ____________ 13. Select ACQUIRE and process at least 1000 events. 14. Record the Means and C.V.s of the beads. If not within specification, troubleshoot instrument and repeat steps 2.6 through 2.8. 15. Print the display screen and attach to this document. Particle Lot No: Expiration Date: Mean TOF EXT FLU1 FLU2 __________ __________ __________ __________ __________ __________ Spec 15 - 25 28 - 54 7 - 73 7 - 73 C.V. % TOF EXT FLU1 FLU2 __________ __________ __________ __________ Spec ≤ 9.0 ≤ 11.0 ≤ 20.0 ≤ 20.0 16. Clean sample cup, sample valve and flow cell following the procedure outlined under the Performance Set Up step number 3 found in the COPAS Instrument Test section of this Service Manual. Union Biometrica, Inc. Confidential www.unionbio.com 21 Performance Verification 1. Place approximately 40mL of sample (C. elegans sample is recommended) into the primary sample cup and cap firmly. 2. Open the FILE Menu and select the 96serp file. 3. Set the instrument sort delay and sort width: a. Select number of events to be sorted per well by entering the desired value (between 0 and 50,000) in the Per Well field located on the main software screen. b. Set the Sort Delay by starting with a recommended value of 8 and adjust the setting up or down in increments of 0.5 until optimal sort results are achieved. c. Set the Sort Width by starting with a recommended value of 5 and adjust the setting up or down in increments of 1.0 until optimal sort results are achieved. NOTE: The sort width must be less than the sort delay. d. Ensure Coincidence Check checkbox is checked, indicating it is ON if desired. 4. Set the following GAINS and SORT PARAMETERS and record actual values (if applicable). Full Scale Gains Actual TOF EXT FLU1 FLU2 256 256 or 512 256 or 512 256 or 512 ________ ________ ________ PMT Control Green Actual 500-600 ________ Yellow N/A EXT FLU1 FLU2 Signal 80 80 80 Actual N/A Integral 100 200 200 Red 600-700 Thresholds Signal 50 TOF Minimum 20 Actual ________ 5. Adjust the sort parameters to the following: Sort Delay Sort Width Per Well Coincidence Sort Parameters 8 ± 1.0 5-7 1 ON ________ ________ 6. Ensure 10 ± 2 events (worms)/sec. If rate is above this target, dilute sample to achieve this rate. 7. Print screens of dot-plots showing TOF vs. EXT and EXT vs. FLU. Union Biometrica, Inc. Confidential www.unionbio.com 22 8. Adjust markers on EXT vs. FLU to gate approximately 60% of the worms. Record actual value. Gate Value (%) ____________% Record the Sort Region: Low # of Events _______ TOF _______ EXT _______ High _______ _______ FLU1 _______ _______ FLU2 _______ _______ 9. Place 96 well tray onto stage and Select LOAD PLATE. 10. Select FILL PLATE and start timer. Upon completion of plate filling, record the time in seconds. Time (S) Specification ____________ ≤ 105 Seconds 11. Print the display screen and attach to this document. 12. View full tray with a microscope using a 4X or 10X objective. Record results on Worksheet. # of wells with < 1 worm # of wells with > 1 worm Specification ____________ ≤ 2 ____________ ≤3 13. Change “Per Well” to 5 and select ACQUIRE to begin particle flow. 14. Place 96 well tray onto stage and Select LOAD PLATE. 15. Select FILL PLATE and start timer. Upon completion of plate filling, record the time in seconds. Time (S) Specification ____________ ≤ 180 Seconds 16. Print the display screen and attach to this document. 17. View first 24 wells with a microscope using a 4X or 10X objective. Record results on Worksheet. Refer to Section 7.13, Sort Parameters in the Operator’s Manual to learn more and if results are not within specifications. Specification # of wells with < 5 worms ____________ ≤2 # of wells with > 5 worms ____________ ≤3 Testing Performed By: _________________________ Date Performed: _________________________ Union Biometrica, Inc. Confidential www.unionbio.com 23 Sorter Operation Verification 1. Turn Sheath and Sample Valves ON. 2. Set SORTER Pressure to 3.0. 3. Place a microscope slide under the sort hole on the waste tray and run for a minimum of 1 minute. 4. Inspect the microscope slide for spray. 5. Spray Visible Yes / No If No, proceed to step 6. If yes, follow the below troubleshooting steps. a. b. c. d. e. Check sheath and sample containers. Verify they are filled appropriately. Check sheath and sample pressures. Verify they have reached the specified levels. Check the waste container. Verify it is draining properly. Check the waste tray. Verify it is pushed all the way to the right. Check the outlet nozzle. Verify that liquid has not accumulated around the nozzle. If it has, perform the following: • Remove the waste tray. • Soak up excess liquid around the nozzle with the tip of a paper tissue. • Return the waste tray to position, observe if spray is still present. f. Check the flow cell for a clog. Clear the flow cell by performing the following: • Click the CLEAN button several times. • Place a finger over the vent on the sheath bottle cap and then remove it, this action will temporarily increase sheath pressure. Observe pressures while performing this action and be sure not to exceed recommended pressures. • Turn sample valve off and click the sheath valve several times, opening and closing the valve. Often these steps are effective in dislodging the clog and re-starting the flow. g. Use the stylus to remove any potential clogs in the flow cell and in the case that previous steps failed to remove the clog. NOTE: Always inspect stylus prior to inserting into the flow cell as damage may result if the stylus is bent or cut. • Remove sample tubing from the flow cell, taking care to not pull on the flow cell. • Insert the stylus into the flow cell, while gently twisting. If resistance is felt STOP, lift the stylus approximately 2mm and insert the stylus again. Repeat this as necessary until the stylus exits the flow cell without any resistance. • Raise and lower the stylus a few times while making small circles with the stylus at the bottom of the flow cell. • Remove the stylus and reconnect the sample tubing. • Select the CLEAN button 2 – 3 times. h. If this procedure has not eliminated the visible spray, please contact factory for assistance. 6. Check the drops for evidence of large worms using a microscope. Union Biometrica, Inc. Confidential www.unionbio.com 24 Instrument Preparation 1. Remove sample from primary sample cup. 2. Add 40ml of COPAS Cleaning Solution to the primary sample cup and cap firmly. 3. Select Sample Valve for approximately 5 minutes. 4. Rinse primary sample cup with distilled water twice. 5. Fill primary sample cup with distilled water and cap firmly. 6. Select Sample Valve for approximately 5 minutes. 7. Fill sample with distilled water and close cap. 8. Close software and turn off power to system and components. Installation Performed By: Date: _________________________ _________________________ Union Biometrica, Inc. Confidential www.unionbio.com 25 Instrument Test Worksheets Sort Delay Value 1 2 3 4 5 6 7 8 9 10 11 12 A Sort Delay B Date ________ C Technician ________ D E F G H 1 2 3 4 5 6 7 8 9 10 11 12 A 1 / Well B Date _________ C Technician _________ D E F G H 1 2 3 4 5 6 7 8 9 10 11 12 A 5 / Well B Date _________ Technician _________ Union Biometrica, Inc. Confidential www.unionbio.com 26 Daily / Short Term COPAS Instrument Maintenance The following Daily Maintenance procedure (also found in the Operator’s Manual) should be performed by the Operator at least once daily when the instrument in use. If the COPAS BIOSORT will be idle for one to two weeks, follow the short term maintenance schedule outlined in the Operator’s Manual. If the COPAS BIOSORT will be idle for greater than two weeks, it is recommended that the short term maintenance schedule also be followed. The following maintenance worksheets are found in the APPENDIX section of the Operator’s Manual. • • • Daily Performance Log Sheet Maintenance Log Sheets: Daily, Short / Long Term Shutdown, Sterilization Troubleshooting Log Sheet Union Biometrica recommends that these be used to store important instrument information. Recording performance data regularly can aid in the identification of subtle shifts in readings, which may indicate system problems. Please make copies of these maintenance worksheet pages as needed. Daily Maintenance Procedure NOTE: Do NOT leave sample or Cleaning Reagent in the instrument over night. 1. Click OFF the SAMPLE VALVE checkbox. 2. Detach the sample tubing and remove cap from primary sample cup. 3. Discard any remaining sample. 4. Fill primary sample cup with approximately 25 mL of Cleaning Reagent. Replace the cap and firmly tighten. 5. Clean the secondary sample container, if used, as follows: a. Add 200 mL of Cleaning Reagent to the secondary sample container. b. Detach the aerators and re-cap the container. c. Shake the container to clean the interior surfaces. d. Empty the Cleaning Reagent from container and rinse the container thoroughly with deionized/distilled water. Visually inspect the container for any remaining sample residue. e. Add 200 mL of deionized/distilled water to the container. f. Clean and reattach the aerators. Replace the cap and firmly tighten. NOTE: Do not put Cleaning Reagent into the secondary sample container while pressure is on. Foaming will occur. NOTE: The secondary sample container may also be washed in a dishwasher or autoclaved if preferred. Union Biometrica, Inc. Confidential www.unionbio.com 27 6. Attach sample tubing to the primary sample cup. 7. Re-check the SAMPLE VALVE checkbox. The warning message, “Caution, this Operation will contaminate the Flow Cell if Sample is Present”, will appear. Click OK. 8. Process the Cleaning Reagent until the sample cup is empty. 9. Click OFF the SAMPLE VALVE checkbox. 10. Detach the sample tubing and remove the cap from primary sample cup. 11. Fill the primary sample cup with 40 mL of deionized/distilled water. 12. Aspirate deionized/distilled water and discard. 13. Fill primary sample cup again with 40 mL of deionized/distilled water and firmly tighten the cap. 14. Attach sample tubing to the primary sample cup. 15. Select Acquire and process the deionized/distilled water for approximately 10 minutes. Do not allow the sample cup to run dry. 16. Click the STOP button twice. 17. Clean external surfaces of any spillage using a water dampened lint free cloth. NOTE: Do not remove or clean any of the electrical connectors or cables of the COPAS BIOSORT. Visual Tubing Check Depending on the frequency of instrument use, it is recommended that a visual inspection of the system and tubing be made. If the instrument is used daily, it is recommended that the external tubing be inspected on a weekly basis, as this tubing will wear out through normal usage. Check the tubing for leaks, specifically around tubing joints. If you determine during the inspection that tubing looks worn or brittle, carefully remove it and replace it with new tubing. Once this has been done, perform the Fluidics Check found in the Final Set-Up / Alignment Procedures section of this Service Manual. Union Biometrica, Inc. Confidential www.unionbio.com 28 Annual Preventative Maintenance Procedure (Revised 12-01-2001) A trained Union Biometrica, Inc. Service Engineer typically performs this Preventative Maintenance Procedure at the annual preventative maintenance visit. As specified by the COPAS instrument warranty agreement, this procedure will be performed by Union Biometrica, Inc. at the end of the one year warranty period and/or at the end of a one year service contract period. Union Biometrica, Inc. recommends that this Preventative Maintenance Procedure be performed annually to maintain optimum instrument performance. Customer Site: __________________________________________ Serial numbers of all system components: ________________________________ Check off box when task is completed Run control Beads and record results at time of arrival to set base line. CVs MEAN EXT TOF GRNFL YELFL REDFL If system does not meet final test specifications, repair and then continue. Replace all sample tubing. Replace all o-rings on sample cup. Replace sample air tubing or sample air fittings. Check all tubing for leaks and wear. Run DI water as sample and check back round counts. Run control Beads and record results. CVs MEAN EXT TOF GRNFL YELFL REDFL Union Biometrica, Inc. Confidential www.unionbio.com 29 Check off box when task is completed Test sorting and record results. Sorted Sample Type__________ Drop Delay ___ Sort Width ___ Coincidence ___ Sort 10 events for 12 wells. Record results: _________________________ Sort 1 event for 96 wells Record results: ___________________________ Check the performance and alignment of the Reflx option. Sort 20 Events in every other well for 24 wells Performance: % Yield % Carry-over % Left in well Measure laser output of each laser. He NE ____mw Argon____mw other _____mw Measure Laser current at operating power. Current________________________amps Replace air filter, check the performance and oil level on the air compressor. Computer 1. Remove all old files. 2. Run defragment program on the C: drive. 3. Conform that there is at lease 500 Megs of disk space free. Remove waste tray and clean. Check waste tray and diverter value alignment. Check the performance and alignment of X-Y stage. Check the performance of twister and confirm twister alignment. Clean outer surfaces of complete system. If system is not performing to final test specifications then repair, return to step 2. Complete Field Service Report. Customer Signature: Date: FSE Performing PM: Date: Union Biometrica, Inc. Confidential www.unionbio.com 30 System Interconnect Drawings COPAS BIOSORT System Interconnect Top View COPAS BIOSORT System Interconnect Internal COPAS Connector Panel COPAS Fluidic Diagram Drawings matching these part numbers / descriptions immediately following this page. Union Biometrica, Inc. Confidential www.unionbio.com 31 5 4 3 2 1 D D IBM PC C C COPAS SYSTEM DISPLAY RUNNING HOUSE AIR SER 066-0009-010 COM RS-232 CABLE #1 WINDOWS IEC-320 LASER C14 Inlet KEYBOARD AC CIRCUIT #1 Dedicated Line 310-5018-000 MOUSE METAL-CLAD FIBER-OPTIC B B CABLE CONTROL ARGON LASER INCLUDES SPECTRA-PHYSICS MODEL 160-SERIES AIR-COOLED ION LASER INCLUDES 310-5010-001 LASER INTERFACE A AC CIRCUIT #2 Dedicated Line IEC-320 A C-20 Inlet 5 4 3 2 1 COPAS BIOSORT SYSTEM INTERCONNECT TOP VIEW 5 4 3 X STEPPER 2 350-5009-001 350-5010-000 1 350-5008-001 350-5011-000 D D EXTINCTION PMT/HV ASSY DETECTOR Y STEPPER WASTE PUMP RED Laser Diode STEPPER 350-5002-001 BULKHEAD 335-5064-000 Blaster Valve CONNECTOR 350-5001-001 C C SORTER CONTROLLER 300-5055-000 Valve Assy's B B CONDOR GMP130E 300-5048-000 POWER SUPPLY STAGE ASSEMBLY (INCLUDES 3005019-000 C/A) CORCOM 3EP1 FILTER 310-5018-000 To Argon Laser Assy IEC-320 AC LINE C14 Inlet A A To PC COM PORT CONNECTOR 5 4 3 2 1 COPAS BIOSORT SYSTEM INTERCONNECT INTERNAL UNION BIOMETRICA, INC. LAST UPDATED 1/21/03 Motor #2 Aux Mixers Sample Valve Stage 25 pin 25 pin 25 pin J18 J14 J7 9 pin Valve Panel 15 pin 15 pin J9 EXT Laser J8 J16 Motor #3 9 pin Diode Laser 5 26 pin Reflex Power 9 pin PMT Assembly J15 COPAS BIOSORT CONNECTOR PANEL (FRONT VIEW) General Parts List The following general parts list includes all assemblies that comprise the COPAS BIOSORT, part number 350-5000-000. For a specific bill of material for an assembly, please contact Union Biometrica, Inc. directly. Part No. Description 350-5000-000 COPAS BIOSORT 300-5036-000 300-5037-000 300-5059-000 300-5061-000 300-5065-000 350-1001-000 350-1002-000 350-5020-000 350-5027-000 350-5041-000 350-5039-000 350-5021-000 350-5023-000 300-5018-000 340-5000-000 340-5001-000 Air / Regulator Filter, Assembly Valve Panel, Assembly Sample Bottle, Assembly Waste Tray, Assembly Waste Pump, Assembly Back Plate Base Plate Final Optics, Assembly System Electronics, Assembly Sample Bottle Support, Assembly Sample Cup, Assembly Argon Laser System, Assembly Laser Cover, Assembly XY-Stage, Assembly ReFlex Sampler, Assembly (Optional) ReFlex Sampler Valve Panel, Assembly (Optional) Drawings matching these part numbers / descriptions immediately following this page. Union Biometrica, Inc. Confidential www.unionbio.com 32 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC. AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA CORP. AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA CORP. WHICHEVER IS FIRST. REV 1 DATE REVISIONS & DESCRIPTION INITIAL WORKING DRAWING 11/01/01 MADE BY APPROVED J.M.M. XXX 10 5 20 34 12 50 65 114 66 31 6 6 ITEM NO. QTY. 5 1 6 1 10 1 11 1 12 1 20 1 21 1 31 1 34 1 50 1 65 1 66 1 76 1 113 1 114 1 21 UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES DRAWN TOLERANCE ON DECIMALS FRAC ENGINEER .XX .XXX .010 .005 ~ ANGLES 30' CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ALL FINISHED SURFACES TO BE 32 MICROINCHES UNLESS NOTED. 113 MATERIAL 350-5000-000 APPLICATION WHERE USED NONE FINISH NONE PART NO. 300-5036-000 300-5037-000 300-5059-000 300-5061-000 300-5065-000 350-1001-000 350-1002-000 350-5020-000 350-5027-000 350-5041-000 350-5039-000 350-5023-000 300-5018-000 340-5000-000 340-5001-000 J.MESSINA KASSEY APPROVED SOMERVILLE, MA. (617) 547-7703 11/01/01 . QUALITY ASSUR UNION BIOMETRICA INC. 11/01/01 . PROJECT PROJECT ENGR DESCRIPTION AIR/REGULATOR FILTER, ASSY VALVE PANEL, ASSY SAMPLE BOTTLE, ASSY WASTE TRAY, ASSY WASTE PUMP, ASSY BACK PLATE BASE PLATE FINAL OPTICS, ASSY SYSTEM ELECTRONICS, ASSY SAMPLE BOTTLE SUPPORT, ASSY SAMPLE CUP, ASSY LASER COVER, ASSY XY-STAGE, ASSY REFLEX SAMPLER, ASSY (OPTIONAL) REFLEX VALVE PANEL, ASSY (OPTIONAL) TITLE: COPAS BIOSORT 250f QUALITY ADDITIONAL COMMENTS: X REV SIZE D 350-5000-000 SCALE 1 : 1 DO NOT SCALE THIS DRAWING 1 SHEET 1 OF 1 REV THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. 1 REVISIONS & DESCRIPTION DATE INITIAL WORKING DRAWING MADE BY APPROVED 06/12/02 . 4 5 1 3 2 ITEM NO. QTY. PART NO. 2 1 119-0002-001 1 1 119-0003-000 3 1 140-0002-000 4 1 140-0002-001 5 1 140-0002-002 DESCRIPTION FITTING, THREADED ELBOW FITTING, THREADED BARB FILTER/REGULATER, AIR PRESSURE BRACKET, AIR FILTER PANEL NUT, AIR FILTER UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES TOLERANCE ON DECIMALS FRAC ANGLES + .XX - .010 + ~ + + - 30' .XXX - .005 DRAWN ENGINEER APPROVED PROJECT . ENGR J.MESSINA 06/12/02 KASSEY 06/12/002 . CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ADDITIONAL COMMENTS: ALL FINISH SURFACES TO BE 32 X MICRO INCHES UNLESS NOTED. MATERIAL TOP LEVEL APPLICATION WHERE USED NONE FINISH NONE . UNION BIOMETRICA INC. SOMERVILLE, MA. (617) 547-7703 TITLE: QUALITY A.NAME ASSUR AIR/REGULATOR, FILTER, ASSEMBLY SIZE REV PART NUMBER: C SCALE:NONE 300-5036-000 DO NOT SCALE THIS DRAWING 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REV 1 . UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES. TOLERANCE ON DECIMALS FRAC ANGLES .010 .005 ~ 30' DRAWN J.MESSINA ENGINEER . APPROVED . CONCENTRICITY .005 TIR. QUALITY . REMOVE BURRS & SHARP EDGES. PROJ. ASSUR. ENG. ALL FINISH SURFACES TO BE 32 MICROINCHES UNLESS NOTED. ADDITIONAL COMMENTS: 350-5000-000 MATERIAL: APPLICATION WHERE USED FINISH: NONE NONE . MADE BY APPROVED . 04/24/03 J.M.M. . . INITIAL WORKING DRAWING . .XX .XXX DATE REVISIONS & DESCRIPTION UNION BIOMETRICA INC. 04/24/03 . . . SOMERVILLE, MA. (617) 591-1211 TITLE: ASSEMBLY, . VALVE PANEL SIZE PART NUMBER: 300-5037-000 B SCALE: 1:2 DO NOT SCALE THIS DRAWING REV 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REVISIONS & DESCRIPTION REV 1 UNLESS OTHERWISE SPECIFIED DRAWN DIMENSIONS ARE IN INCHES ENGINEER TOLERANCE ON DECIMALS FRAC ANGLES APPROVED .XX + - .010 + ~ + - 30' .XXX + .005 - CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ALL FINISH SURFACES TO BE 32 MICRO INCHES UNLESS NOTED. MATERIAL 350-5000-000 APPLICATION WHERE USED NONE FINISH NONE PROJECT . ENGR DATE 07/18/02 J.M.M. INITIAL WORKING DRAWING J.MESSINA 07/18/02 KASSEY 07/18/02 . . MADE BY APPROVED . UNION BIOMETRICA INC. SOMERVILLE, MA. (617) 547-7703 TITLE: ASSEMBLY, SAMPLE BOTTLE QUALITY . ASSUR ADDITIONAL COMMENTS: X SIZE PART NUMBER: 300-5059-000 C SCALE: 1:1 DO NOT SCALE THIS DRAWING REV 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REV B . UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES. TOLERANCE ON DECIMALS FRAC ANGLES 370-5000-000 350-5000-000 335-5000-000 APPLICATION WHERE USED .010 .005 ~ 30' DRAWN J.MESSINA ENGINEER KASSEY APPROVED . CONCENTRICITY .005 TIR. QUALITY . REMOVE BURRS & SHARP EDGES. PROJ. ASSUR. ENG. ALL FINISH SURFACES TO BE 32 MICROINCHES UNLESS NOTED. ADDITIONAL COMMENTS: MATERIAL: FINISH: NONE NONE . MADE BY APPROVED . 07/19/02 J.M.M. . . INITIAL WORKING DRAWING . .XX .XXX DATE REVISIONS & DESCRIPTION . 07/19/02 UNION BIOMETRICA INC. 07/19/02 SOMERVILLE, MA. (617) 547-7703 . TITLE: WASTE TRAY, . ASSEMBLY SIZE PART NUMBER: 300-5061-000 B SCALE: 1:1 DO NOT SCALE THIS DRAWING REV B SHEET 1 OF 1 REV THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. 1 REVISIONS & DESCRIPTION DATE INITIAL WORKING DRAWING 06/11/02 MADE BY APPROVED J.M.M. 14 12 15 10 13 5 4 ITEM NO. QTY. PART NO. DESCRIPTION 4 1 070-0020-002 MOTOR, STEPPER 5 4 131-0440-009 SCREW, PH, #4-40 X 7/16" 6 2 131-0832-001 SCREW, PH, #8-32 X 1/2" 10 3 139-0003-001 MOUNT, ISOLATION 11 4 300-1061-000 PIN, ROLLAR 12 1 300-1062-000 MOUNT, PUMP MOTOR 13 1 300-1063-000 PLATE, PUMP SUPPORT 14 4 300-1064-000 ROLLER, PUMP 15 1 350-1022-000 ROTOR UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES TOLERANCE ON DECIMALS FRAC ANGLES + .XX - .010 + ~ + + - 30' .XXX - .005 DRAWN ENGINEER J.MESSINA 06/11/02 KASSEY 06/11/002 APPROVED PROJECT A.NAME ENGR . CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ADDITIONAL COMMENTS: ALL FINISH SURFACES TO BE 32 X MICRO INCHES UNLESS NOTED. MATERIAL TOP LEVEL APPLICATION WHERE USED NONE FINISH NONE . UNION BIOMETRICA INC. SOMERVILLE, MA. (617) 547-7703 TITLE: WASTE PUMP QUALITY A.NAME ASSUR ASSEMBLY SIZE REV PART NUMBER: C SCALE: NONE 300-5065-000 DO NOT SCALE THIS DRAWING 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC. AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC. AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER IS FIRST. REV 1 DATE REVISIONS & DESCRIPTION INITIAL WORKING DRAWING 06/26/01 MADE BY APPROVED J.M.M. 6 4 5 ITEM NO. 4 5 6 QTY. 1 1 1 PART NO. 350-5014-000 350-5006-000 350-5005-000 DESCRIPTION UNION BIOMETRICA INC. ENGINEER KASSEY 06/26/01 SOMERVILLE, MA. (617) 547-7703 APPROVED . TOLERANCE ON DECIMALS FRAC .010 .005 ~ ANGLES 30' 360-5000-000 CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ALL FINISH SURFACES TO BE 32 MICRO INCHES UNLESS NOTED. 350-5000-000 MATERIAL 335-5000-000 APPLICATION WHERE USED 06/26/01 DRAWN .XX .XXX 370-5000-000 J.MESSINA UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES NONE FINISH NONE PROJECT D.PERRAULT ENGR . ASSEMBLY, FINAL OPTICS QUALITY . ASSUR ADDITIONAL COMMENTS: X REV SIZE 350-5020-000 SCALE 1 : 1 DO NOT SCALE THIS DRAWING 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REVISIONS & DESCRIPTION REV 1 UNLESS OTHERWISE SPECIFIED DRAWN DIMENSIONS ARE IN INCHES ENGINEER TOLERANCE ON DECIMALS FRAC ANGLES APPROVED .XX + - .010 + ~ + - 30' .XXX + .005 - CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ALL FINISH SURFACES TO BE 32 MICRO INCHES UNLESS NOTED. MATERIAL 350-5000-000 APPLICATION WHERE USED NONE FINISH NONE PROJECT . ENGR DATE 04/22/03 J.M.M. INITIAL WORKING DRAWING J.MESSINA . . . UNION BIOMETRICA INC. 04/21/03 SOMERVILLE, MA. (617) 591-1211 . . MADE BY APPROVED TITLE: QUALITY . ASSUR ADDITIONAL COMMENTS: ASSEMBLY, SYSTEM ELECTRONICS PANEL X SIZE PART NUMBER: 350-5027-000 C SCALE: 1:2 DO NOT SCALE THIS DRAWING REV 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REV 1 3 DATE REVISIONS & DESCRIPTION . 07/24/02 J.M.M. . . INITIAL WORKING DRAWING . . MADE BY APPROVED . 2 4 UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES. TOLERANCE ON DECIMALS FRAC ANGLES .XX .XXX ITEM NO. QTY. PART NO. DESCRIPTION 1 1 300-1042-000 RING, SAMPLE BOTTLE SUPPORT 2 1 350-1003-000 PLATE, SAMPLE BOTTLE 3 2 300-1044-000 BRACKET, SUPPORT 360-5000-000 .010 .005 30' J.MESSINA ENGINEER KASSEY APPROVED . CONCENTRICITY .005 TIR. QUALITY . REMOVE BURRS & SHARP EDGES. PROJ. ASSUR. ENG. ALL FINISH SURFACES TO BE 32 MICROINCHES UNLESS NOTED. ADDITIONAL COMMENTS: 350-5000-000 MATERIAL: APPLICATION WHERE USED ~ DRAWN FINISH: NONE NONE . 07/24/02 UNION BIOMETRICA INC. 07/24/02 SOMERVILLE, MA. (617) 547-7703 . TITLE: SUPPORT ASSEMBLY, . SAMPLE BOTTLE SIZE PART NUMBER: 350-5041-000 B SCALE: NONE DO NOT SCALE THIS DRAWING REV 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REV 1 . UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES. TOLERANCE ON DECIMALS FRAC ANGLES .010 .005 ~ 30' DRAWN J.MESSINA ENGINEER KASSEY APPROVED . CONCENTRICITY .005 TIR. QUALITY . REMOVE BURRS & SHARP EDGES. PROJ. ASSUR. ENG. ALL FINISH SURFACES TO BE 32 MICROINCHES UNLESS NOTED. ADDITIONAL COMMENTS: 350-5000-000 MATERIAL: APPLICATION WHERE USED FINISH: NONE NONE . MADE BY APPROVED . 07/15/02 J.M.M. . . INITIAL WORKING DRAWING . .XX .XXX DATE REVISIONS & DESCRIPTION UNION BIOMETRICA INC. 07/15/02 07/15/02 . . SOMERVILLE, MA. (617) 591-1211 TITLE: ASSEMBLY, . SAMPLE CUP SIZE PART NUMBER: 350-5039-000 B SCALE: 1:2 DO NOT SCALE THIS DRAWING REV 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REVISIONS & DESCRIPTION REV 1 UNLESS OTHERWISE SPECIFIED DRAWN DIMENSIONS ARE IN INCHES ENGINEER TOLERANCE ON DECIMALS FRAC ANGLES APPROVED .XX + - .010 + ~ + - 30' .XXX + .005 - CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ALL FINISH SURFACES TO BE 32 MICRO INCHES UNLESS NOTED. MATERIAL ALL APPLICATION WHERE USED NONE FINISH NONE PROJECT . ENGR DATE 04/22/02 J.M.M. INITIAL WORKING DRAWING J.MESSINA 04/22/02 KASSEY 04/22/02 . . MADE BY APPROVED . UNION BIOMETRICA INC. SOMERVILLE, MA. (617) 591-1211 TITLE: ASSEMBLY, ARGON LASER SYSTEM QUALITY . ASSUR ADDITIONAL COMMENTS: X SIZE PART NUMBER: 350-5021-000 C SCALE: 1:3 DO NOT SCALE THIS DRAWING REV 1 SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. REV 1 . UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES. TOLERANCE ON DECIMALS FRAC ANGLES .010 .005 ~ 30' DRAWN J.MESSINA ENGINEER . APPROVED . CONCENTRICITY .005 TIR. QUALITY . REMOVE BURRS & SHARP EDGES. PROJ. ASSUR. ENG. ALL FINISH SURFACES TO BE 32 MICROINCHES UNLESS NOTED. ADDITIONAL COMMENTS: 350-5000-000 MATERIAL: APPLICATION WHERE USED FINISH: NONE NONE . MADE BY APPROVED . 02/05/03 J.M.M. . . INITIAL WORKING DRAWING . .XX .XXX DATE REVISIONS & DESCRIPTION UNION BIOMETRICA INC. 02/05/03 . . . SOMERVILLE, MA. (617) 591-1211 TITLE: ASSEMBLY, . LASER COVER SIZE PART NUMBER: 350-5023-000 B SCALE: 1:2 DO NOT SCALE THIS DRAWING REV 1 SHEET 1 OF 1 REV THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC., AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC., AND SHALLNOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER COMES FIRST. C REVISIONS & DESCRIPTION DATE INITIAL WORKING DRAWING MADE BY APPROVED 06/03/02 . 32 32 11 12 16 8 21 18 35 14 11 10 18 16 16 18 9 21 13 51 19 17 31 17 10 14 17 17 ITEM NO. QTY. PART NO. DESCRIPTION 8 2 300-1001-000 BRACKET, LEFT SIDE MOUNTING 9 2 300-1002-000 BRACKET, RIGHT SIDE MOUNTING 10 2 300-1003-000 ROD, Y-STAGE TRAVERSE 11 2 300-1004-000 ROD, Y-STAGE TRAVERSE 12 1 300-1005-000 BRACKET, Y-STAGE TOP MOUNTING 13 1 300-1006-000 BRACKET, X-STAGE TOP MOUNTING 14 2 300-1009-000 BRACKET, MOTOR MOUNTING 16 4 300-1011-000 BEARING, LARGE MOTOR MOUNTING 17 4 300-1012-000 BEARING, SMALL MOTOR MOUNTING 18 4 300-1013-000 VANE, HALL-EFFECT 19 1 300-1014-000 BASEPLATE, X-STAGE 20 1 300-1046-000 COVER, X-STAGE CONNECTOR 32 2 300-1100-000 PLATE, LOCATOR 51 1 300-1103-000 COVER, PCB, X-Y STAGE 21 4 300-5011-000 ASSEMBLY, PCB, LIMIT SENSOR 31 1 300-5024-000 ASSEMBLY, MOTOR, Y-STAGE 35 1 300-5025-000 ASSEMBLY, MOTOR, X-STAGE 8 20 9 21 UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES TOLERANCE ON DECIMALS FRAC ANGLES .XX + - .010 + ~ + + .005 - 30' .XXX - DRAWN J.MESSINA 06/03/02 ENGINEER KASSEY 06/03/02 APPROVED . . PROJECT MATERIAL TOP LEVEL APPLICATION WHERE USED FINISH NONE NONE SOMERVILLE, MA. (617) 547-7703 TITLE: X-Y STAGE ASSEMBLY QUALITY . ASSUR . ENGR CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ADDITIONAL ALL FINISH SURFACES TO BE 32 X MICRO INCHES UNLESS NOTED. UNION BIOMETRICA INC. COMMENTS: SIZE REV PART NUMBER: C SCALE:NONE 300-5018-000 DO NOT SCALE THIS DRAWING C SHEET 1 OF 1 THIS DRAWING IS THE SOLE PROPERTY OF UNION BIOMETRICA INC. AND SHALL NOT BE REPRODUCED IN ANY FORM WHATSOEVER. THE CONTENTS ARE FOR THE PURPOSES ONLY OF QUOTATION TO OR MANUFACTURE FOR UNION BIOMETRICA INC. AND SHALL NOT BE USED AS A BASIS FOR QUOTATION TO OR MANUFACTURE FOR OTHERS. THIS DRAWING SHALL BE RETURNED WITH QUOTATION OR AT TERMINATION OF MANUFACTURING CONTRACT OR ON DEMAND OF UNION BIOMETRICA INC., WHICHEVER IS FIRST. REV 1 18 DATE REVISIONS & DESCRIPTION INITIAL WORKING DRAWING 11/30/01 MADE BY APPROVED J.M.M. 27 19 28 24 26 9 32 22 30 20 23 ITEM NO. QTY. PART NO. 9 1 300-1013-000 18 1 340-1006-000 19 1 340-1001-000 20 1 340-1002-000 21 1 340-1004-000 22 1 340-1007-000 23 1 340-1003-000 24 1 340-1012-000 26 1 300-5011-000 27 2 340-1010-000 28 1 340-1005-000 29 1 070-0040-002 30 1 199-0006-002 31 1 199-0006-001 32 1 120-0003-003 33 1 340-1008-000 21 29 11/30/01 UNION BIOMETRICA INC. ENGINEER KASSEY 11/30/01 SOMERVILLE, MA. (617) 547-7703 APPROVED . DRAWN TOLERANCE ON DECIMALS FRAC .XX .XXX .010 .005 ~ ANGLES 30' CONCENTRICITY .005 TIR REMOVE BURRS & SHARP EDGES ALL FINISH SURFACES TO BE 32 MICRO INCHES UNLESS NOTED. MATERIAL 340-5000-000 APPLICATION WHERE USED J.MESSINA UNLESS OTHERWISE SPECIFIED DIMENSIONS ARE IN INCHES NONE FINISH NONE PROJECT . ENGR . QUALITY A.NAME ASSUR SAMPLER, ASSEMBLY ADDITIONAL COMMENTS: X REV SIZE C 340-5000-000 SCALE 1 : 2 DO NOT SCALE THIS DRAWING 1 SHEET 1 OF 1 Printed Circuit Boards Assembly # Description 310-5010-000 340-5005-000 350-5001-000 350-5002-000 350-5008-000 350-5009-000 Laser Interface ReFlx Sampler Sorter Controller Bulkhead Connector Extinction Detector PMT Preamp/HV Board Revision No. Rev B Rev B Rev D Rev A Rev A Rev A Parts List Revision No. Rev C2 Rev 4 Rev D1 Rev A3 Rev A3 Rev A1 Notes ReFlx option Drawings matching these part numbers / descriptions immediately following this page. Major Cable Assemblies Assembly # Description 300-5019-000 300-5048-000 300-5055-000 310-5018-000 335-5064-000 340-5006-000 340-5007-000 350-5010-000 350-5011-000 350-5012-000 350-5013-000 XY Internal C/A Stage C/A Valve Panel C/A Laser Control Blaster C/A Frontside C/A Backside C/A PMT C/A Detector C/A Stirrer C/A Sheath C/A Union Biometrica, Inc. Confidential www.unionbio.com Board Revision No. Rev A Rev A Rev A Rev A Rev A Rev 5 Rev 7 Rev 1 Rev 2 Rev 2 Rev 2 Parts List Revision No. Rev A Rev A Rev A Rev A Rev A Rev 5 Rev 5 Rev 1 Rev 2 Rev 2 Rev 2 Notes ReFlx option ReFlx option 33 8 7 6 5 4 3 2 1 REV ECN NO. APPROVAL DATE STANDARD JUMPER CONFIGURATION VCC1 JP1 Not Installed, JP1: (External Interlock Switch Active) NOT installed JP2 Installed. P6 TO INTERLOCK SWITCH JP2: (Ignore 'Interlock OK') INSTALLED REL1 14 1 2 1 2 D6 1N4148 VCC1 CON2 JP1 D GND1 6 7 8 JP2 JP3: (Force Local Mode) INSTALLED OC1 8 7 6 5 R1 1K 2 3 GND2 HCPL-2211 D REED RELAY Force Local Mode (Installed) JP3 INTLK_OFF/ON# GND1 Y1 Y2 Y3 Y4 Y5 Y6 A1 A2 A3 A4 A5 A6 TO LASER POWER SUPPLY GND1 INTLK_OK/OFF# 1K R2 2 3 VCC2 VALVE10 PWR/CUR#_SEL2 VALVE11 STANDBY/RUN2# VALVE12 DIS_OFF/EN2# 1 9 2 10 3 11 4 12 5 13 6 14 7 15 8 HCPL-2211 OC3 8 7 6 5 VCC1 74AHC04 PWR/CUR#_SEL1 GND1 1K R3 2 3 VCC2 LASERMON SET RUN/STANDBY1# HCPL-2211 CURRENT_MONITOR POWER_MONITOR 1Y 1A 1B PWRCTL 5 2Y 2A 2B 1 2 GND1 6 7 VCC2 8 7 6 5 DIS_EN/OFF1# U3 3 OC4 VCC1 STANDBY/RUN1# TO J9 ON BULKHEAD PANEL Via 310-5018-000 C/A (1:1) 1K R4 2 3 DB15 VCC2 1 Via 310-5017-000 Cable P3 8 7 6 5 CUR/PWR#_SEL1 1 3 5 9 11 13 J1 1 9 2 10 3 11 4 12 5 13 6 14 7 15 8 OC2 VCC1 U2 2 4 6 8 10 12 GND2 HCPL-2211 DIS_OFF/EN1# TP6 CON1 SN75462 MONITOR DB15 GND1 GND1 GND2 VCC2 VCC2 GAIN VCC2 R16 499R OC5 GND1 Radded 4 3 2 1 4.99K 4.99K added 6/7/01 VCC to desensitize POT1 R11 adjustment range. C GND1 POT1 200K 10K Q1 2N3906 2 8 R30 VDD VSS VL GND GND2 GND2 NOTE: GND1, GND2, VCC1, VCC2 ARE VCC1 GND1 GND2 R22 499R R24 +12V VCC1 VCC2 VCC2 PWRCTL DVM TP1 CON1 TP2 CON1 TP3 CON1 TP4 CON1 HCNR200 CAL VCC 69.8K Q5 2N3904 Q7 2N3904 Q8 2N3906 POT2 200K Q6 2N3906 GND1 VCC1 GND1 R21 10R DIS_EN/OFF1# C13 .33 C14 .33 C15 .33 C16 .33 C27 .33 C29 R27 10K 4 3 2 1 1 5 6 7 8 R23 10K 1 POT3 200K VCC1 .33 ALL ISOLATED IN CLAD GND2 OC6 GND1 1 2 3 4 2 3 4 GND1 1 1 VCC1 R17 10R 1 Hour Meter ORN BLK WHI RED Q3 2N3906 R13 10R VCC1 P5 1 2 4 5 R12 10K VCC1 GND1 VCC1 GND1 C12 GND2 1 4 7 5 3 C11 .33 C Q4 2N3904 ADG419 CON10 Curtis #701 RR 601 00512D D .33 C17 22 69.8K +12V GND1 VCC1 1 2 3 4 5 6 7 8 9 10 PANEL METER + R14 Q2 2N3904 IN 10K P4 TO DIGITAL S1 S2 6 R15 10K HCNR200 U1 PWR/CUR#_SEL1 5 6 7 8 R25 10R GND1 GND2 GND1 GND1 GND2 GND2 SET GAIN R26 10K VCC1 (See ECO Note) GND1 +12V +12V GND CON4 B SHOWN FOR REFERENCE ONLY ORN 1 RED 5 (NC) 3 2 BLK 4 WHI GND1 GND1 GND2 VCC2 Q12 DIS_EN/OFF1# VCC1 VR1 LM7805 1 +12V 3 OUT VCC1 + GND C22 22 TR Q DIS 8 Q11 2N3904 5 10K GND1 R 2 .01 DIS_OFF/EN1# ("Slo-Blo") with appropriate agency recognition. Variation from these values will result in either unreliable operation, 1N4148 Q13 TP5 CON1 R31 is 200 mA. In both cases, the fuses must be Type T D2 CV THR D3 3 7 1N4148 IRLD024 6 R36 10K R32 GND1 or unsafe operation. C25 .01 10K GND1 The silkscreen text is in error. U4 NE555 C26 220uF GND1 GND1 GND1 GND1 ECO Note: F1 A SEE NOTE 6 RS1A T1 12 4 3 10 9 - + VCC1 3 1 7 5 Union Biometrica Inc. 35 Medford Street, Suite 101 Somerville, MA 02143 LED4 CUR/PWR#_SEL1 6 DATE 330R I8 AMBER R41 LED5 Title GND1 INTLK_OFF/ON# VCC1 DATE 332 7 Laser Interface CHECKED BY CON1 8 Panel. (Conflict with sheath pump motor control) STANDBY/RUN1# AMBER RS1C GT1 EGND it to ground at the J9 connector on the Bulkhead DRAWN BY VCC1 VPP10-3000 6 A drive signal by removing the driven end, and shorting LED3 4 330R I8 C23 1000uF DIS_OFF/EN1# AMBER RS1B +12V + An ECO of May '01 effectively disabled the SET LED2 2 330R I8 GND1 CON6 1 1 RS402LD 6 5 4 3 2 1 AC MAIN VCC1 DB1 P1 TO TO FANS 22uF C24 RS1D 330R I8 R35 1M 1 2 3 CON3 4 1 GND1 100 mA. For 120V systems, the correct amperage rating D5 MUR120 GND1 + R34 10K 10K 7 GND1 +12V VCC1 C28 R33 GND1 F1. For 230V systems, the correct amperage rating is R42 10K VCC1 LED1 GREEN 2 CE compliance requires that the correct fuse be used at P7 +12V VCC1 C21 .33 IMPORTANT FUSE NOTE: IRLD024 GND1 IN B D4 MUR120 Size C Document Number Date: Tuesday, April 29, 2003 5 4 3 2 Rev 310-5010-SC0 AMBER B Sheet 1 of 1 1 COPAS BIOSORT LASER INTERFACE 5 4 3 2 1 +5 2 +24 D1 +3.3 P4 V6# USB Bus COPAS Powered Powered 1 PE[0..7] PE[0..7] +24 R1 42 31 18 7 U2 74LCX16244 PE[0..7] D PB[0..4] PB[0..4] + + + + PE7 PE6 PE5 PE4 47 46 44 43 1 PB[0..4] PB4 41 40 38 37 48 V2# V1# V0# +24 O8 O9 O10 O11 I12 I13 I14 I15 OE4# O12 O13 O14 O15 - RP3 8 7 6 5 19 20 22 23 1.8K I8 1 2 3 4 C17 TP4 120uF/50V 5R U5 74AHCT14 1 3 5 9 11 13 45 - - C23 C18 2 4 6 8 10 12 +3.3 5R OI 5 +5 4 499R LCX14 R3 12 LIMSW ISPOWERED# R16 2 IO 20.0K G C20 0.22/50V R17 LCX14 R14 237R 1 R11 +5 1 LCX14 R100 237R 2 4.99K PB PB Bsen /PB /PB DIR EN PA PA Asen /PA /PA H/F Iadj C14 5R 1 C1+ Vcc 22uF/35V C32 4 5R 5R 0.1 C34 10 9 JP100 PB3 PB2 499R 499R +3.3 X0 A X1 = 340mA/winding TP1 CGRID-8M C8 0.22/50V 0.1 C19 0.22/50V C1 0.22/50V 5R 5R 5R CGRID-4M 5R RIX1 TOX1 1 2 3 4 A MNT1 MNT2 MNT3 MNT4 U3B IB462H 25 24 23 22 1 1 5R 1 CGRID-8M LTC1386CS/MAX3232 P101 Title ReFlx Sampler Board - Peripheral Drivers 5R DO NOT POPULATE Size Date: 4 3 Document Number B 5R 5 CAVRO PUMPS C31 GND 47R 47R +5 2 GND 47R R101 V+ +5 1 2 3 4 5 6 7 8 4.99K R102 V- 1 2 3 4 5 6 7 8 7 8 1 X4 T2in T2out R2out R2in GND X3 R103 T1in T1out R1out R1in P3 +24 14 13 P100 4.99K R104 0.1 C2- GND X2 R105 ALT FUNC B 1 R107 C35 5R 5 15 R106 6 1 2 3 4 C1- GND +3.3 U3A 0.1 3 11 12 TX0 RX0 TP3 Vsetpoint : 170mV 5R 0.1 CGRID-10M PHASE A 1 U11 C2+ C30 5R 16 15 + 0.22/50V +5 19 20 21 17 18 C IB462H 16 C21 C6 0.22/50V 5R PHASE B TP2 1 LIMSW +3.3 B 11 12 8 9 10 /R 10.0K 7 +3.3 Vsetpoint +5 4.99K 3 SClk 1 2 3 4 5 6 7 8 9 10 0V IO 13 2 +5V 0V OI PB7 JP101 JUMPER R12 U10A PB6 10 + 11 1 5R 4.99K BLIMSW 14 U10F LCX14 U10E X1 R13 10.0K +5 PB5 4 TO STEPPER 5R P1 R2 +3.3 0.22/50V 22uF/35V 7 39 34 28 21 15 10 3 CGRID-6M +24 5R +5 7 X0 +5 CGRID-10M 5R PB[5..7] POWER IN 6 5 4 3 2 1 +V I8 I9 I10 I11 OE3# PB[5..7] U10B P2 +24 5R X2 X3 X4 PB3 13 14 16 17 6 PB[5..7] D TO VALVES 1 2 3 4 5 6 7 8 9 10 V3# 14 4 10 COM V6# V5# +V PC[2..5] PC[2..5] V4# 18 17 16 15 14 13 12 11 01 02 03 04 05 06 07 08 13 PC[2..5] 1.8K I8 1 2 3 4 I1 I2 I3 I4 I5 I6 I7 I8 + C 8 9 11 12 RP2 8 7 6 5 1 2 3 4 5 6 7 8 1 30 29 27 26 24 1.8K I8 1 2 3 4 14 PC3 PC4 PC5 PC2 O4 O5 O6 O7 RP1 8 7 6 5 P5 + 36 35 33 32 25 I4 I5 I6 I7 OE2# 2 3 5 6 CGRID-4M 499R 9 PB0 PB1 PB2 O0 O1 O2 O3 0V PE3 PE2 PE1 PE0 VALVEN# PB3 I0 I1 I2 I3 OE1# U1 62081 EXPANDER 1 2 3 4 V5# HEARTBEAT 2 Tuesday, April 29, 2003 Rev 340-5005-SCO Sheet 1 B1 1 of 2 REFLEX SAMPLER OPTIONAL MODULE PAGE 1 5 4 3 2 1 Y1 x1 x2 C25 C26 +3.3 12 MHz 27pF BUFRST# +3.3 R8 5R 5R 1M H2 3 Q1 R4 C28 C16 R20 1M 0.1 GND C GND D 53 54 A GND B GND 8 7 6 5 C9 0.1 C11 0.1 5R 73 74 5R 76 77 5R U6 C15 U8 + 5 8 7 2 0.22/50V 5R 128Kx8 SRAM 5R 8 24 +3.3 MAX882 IN LBI #STBY SET OUT LBO GND GND 4 1 3 6 5 R19 + 100K C29 22uF/35V A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 VDD VDD CE 5R 5R 5R 5R +3.3 RP4 16 15 14 13 12 11 10 9 B 1 2 3 4 5 6 7 8 PB4 H1 AOE# OE# BSEL ASEL XCLK R9 H1 32 WR# 12 28 22.1R 9 25 R22 100K U10C S1 +3.3 5 RP6 1 2 3 4 5 6 7 8 16 15 14 13 12 11 10 9 OI U10D 6 LCX14 SA0 WR# RD# 9 OI 5R LCX14 6 7 10 11 22 23 26 27 D0 D1 D2 D3 D4 D5 D6 D7 8 9 10 11 13 14 15 16 EA XCLK R10 22.1R CLKOUT C33 WAKEUP# +3.3 +3.3 PC0/RxD0 PC1/TxD0 PC2/INT0# PC3/INT1# PC4/T0 PC5/T1 PC6/WR# PC7/RD# A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 C3 C5 0.1 0.1 0.1 0.1 D0 D1 D2 D3 D4 D5 D6 D7 PE0 PE1 PE2 PE3 PE4 PE5 PE6 PE7 BKPT PSEN# adr5 EA XCLK CLKOUT WAKEUP# RDY0/ASEL RDY1/BSEL RDY2/AOE RDY3/BOE RDY4/SLWR RDY5/SLRD CTL0/AINFLAG CTL1/BINFLAG CTL2/AOUTFLAG SCL SDA PA0 PA1 OE# PA3 PA4 PA5 PA6 PA7 79 80 81 82 83 84 85 86 PB0 PB1 PB2 PB3 PB4 PB5 PB6 PB7 110 111 112 113 123 124 125 126 RX0 TX0 PC2 PC3 PC4 PC5 WR# RD# 56 57 58 59 60 61 63 64 PD0 PD1 PD2 PD3 PD4 PD5 PD6 PD7 88 89 90 91 92 93 94 95 PE0 PE1 PE2 PE3 PE4 PE5 PE6 PE7 PB[0..4] PB[0..4] PB[5..7] PC[2..5] PE[0..7] PE[0..7] +3.3 J3 5R 102 103 104 44 45 46 ASEL BSEL AOE# BOE# SLWR SLRD 101 96 97 BINFLAG SCL PA0 PA1 PA3 PA4 PA5 PA6 PA7 2 4 6 8 10 12 14 16 18 20 5R (H1) + + + + + + + + + + + + + + + + + + + + 5R PC5 PC4 PC3 PC2 BSEL BINFLAG SLRD SLWR 1K 2 4 6 8 10 12 14 16 18 20 H3 XCLKSEL 5R 1 3 5 7 9 11 13 15 17 19 SDA XCLK BUFRST# B RD# BOE# 5R CLKOUT 3M-20 +3.3 J2 (H2) + + + + + + + + + + + + + + + + + + + + 1 3 5 7 9 11 13 15 17 19 PD7 PD6 PD5 PD4 PD3 PD2 PD1 PD0 5R A 3M-20 5R 5R 5R USB01 5R Title ReFlx Sampler Board - USB Control 10K I16 Size Document Number B Date: 5 C RX0 TX0 PB[5..7] PC[2..5] PD0 PD1 PD2 PD3 PD4 PD5 PD6 PD7 CY7C64613-128NC 5R EA, DISCON#, PC2 must be pulldowns C4 0.1 22 38 37 39 5R DM5 DP5 SCL SDA 24LC00 390R R15 DM4 DP4 119 99 87 78 72 67 62 52 47 40 35 23 12 3 8 7 6 5 PB0/T2 PB1/T2EX PB2/RXD1 PB3/TXD1 PB4/INT4 PB5/INT5# PB6/INT6 PB7/T2OUT DM3 DP3 21 1 R21 Vcc WP SCL SDA DM2 DP2 25 26 27 28 29 30 31 32 C48TST XCLKSEL TM0 TEST R18 1K U9 A0 A1 A2 Vss USBDUSBD+ Gnd14 Gnd13 Gnd12 Gnd11 Gnd10 Gnd9 Gnd8 Gnd7 Gnd6 Gnd5 Gnd4 Gnd3 Gnd2 Gnd1 0.1 1 2 3 4 "POWER" SLWR SLRD DISCON# H2 EA PC2 C2 0.1 5R PA0/T0out PA1/T1out PA2/OE# PA3/CS# PA4/FWR# PA5/FRD# PA6/RxD0out PA7/RxD1out DISCON# RESET# AGnd BOE# +3.3 C22 5R 41 33 24 51 98 34 7 5 6 5R 2 5R 105 106 107 108 114 115 116 117 118 120 121 122 127 128 1 2 5R SA0 16 15 14 13 12 11 10 9 Vss Vss A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 0.1 WAKEUP# H3 OE# XCLKSEL RP5 1 2 3 4 5 6 7 8 D0 D1 D2 D3 D4 D5 D6 D7 OE 1 2 3 4 13 14 15 16 17 18 19 20 21 29 30 31 BUFRST# 8 D2 A WE RESET 10K I16 +3.3 A16 +3.3 10K I16 70 71 5R C 5R 65 66 +3.3 SN75240PW 5R 0.1 C7 0.1 D 20 1 2 3 4 L2 C13 0.1 XOUT DM0 DP0 AVcc XIN 22.1R 48 69 19 22.1R R6 18 NC2 NC1 R7 U7 USB-B DISCON# BUFRST# 22uF/35V PWR2# OVC2# USBDUSBD+ Avcc + 5R Vcc8 Vcc7 Vcc6 Vcc5 Vcc4 Vcc3 Vcc2 Vcc1 R5 1.5K 43 42 +3.3 50 49 1 1 2 3 4 5 6 109 100 75 68 55 36 17 4 U4 0.1 2N4401 C10 VCC DD+ GND SHELL SHELL C12 DISCON# C27 L1 C24 1K 2 D J1 PLACE ONE CAP PER EACH VCC OF U9 27pF 4 3 2 Rev B1 340-5005-SCO Tuesday, April 29, 2003 Sheet 1 2 of 2 REFLEX SAMPLER OPTIONAL MODULE PAGE 2 8 7 6 5 4 3 2 1 REV DRIVERS HV2_CTL HV3_CTL HV2_CTL HV3_CTL ECN NO. APPROVAL DATE PG 3 HV2C HV3C S2C S3C HV2C HV3C S2C S3C S1_PLS D D VALVE9 I/O BRESET PG 6 S1_PLS VALVE9 /BLAST BOOST /VALVEN VALVLD VALVLD2 /DACLD BRESET X MOTOR PROCESSOR Y MOTOR PROCESSOR PG 8 20MHZ PG 10 XMC[0..2] +3VREF SA[0..2] BRESET SD[0..7] 20MHZ XMC[0..2] XSW[0..1] BRESET XSW[0..1] 20MHZ THRESHOLD NM_DRVRS MULTIPLEXOR & ADC XMC[0..2] YMC[0..2] XSW[0..1] YSW[0..1] YMC[0..2] YMC[0..2] YSW[0..1] YSW[0..1] PG 2 H1 THRESHOLD XTXD0 +3VREF TRCH[0..1] EXTINT FL1INT EXTSIG FL1SIG PRESS1 PRESS2 PRESS3 PRESS4 C FL2INT FL3INT FL2SIG FL3SIG AUX1SIG AUX2SIG AUX3SIG LASERMON EXTINT FL1INT EXTSIG FL1SIG PRESS1 PRESS2 PRESS3 PRESS4 FL2INT FL3INT FL2SIG FL3SIG AUX1SIG AUX2SIG AUX3SIG RXD0 PRESS1 PRESS2 PRESS3 PRESS4 AUX1 AUX2 AUX3 B TXD0 ADCCH[0..3] /ADCSEL ADCHBEN /ADCSTART ADCCH[0..3] YLED H1 XIRQ XIO NM_YPROC BDINPUT[0..3] C YLED XLED SLED NM_XPROC /MCOATHRESH S1_AWOFF /SRD SALE SORTING CONTROL PG 7 SD[0..7] S2_PLS S3_PLS TRCH[0..1] /BLAST BOOST /VALVEN VALVLD VALVLD2 /DACLD PG 4 EXTINT FL1INT S2_AWOFF S3_AWOFF XTXD0 /MCOATHRESH YTXD0 /INTEGRATE /DUMP /INTEGRATE /DUMP PRESS1 PRESS2 PRESS3 PRESS4 /BGAINSEL1 /BGAINSEL2 /BINTSEL1 /BINTSEL2 /BGAINSEL1 /BGAINSEL2 /BINTSEL1 /BINTSEL2 S2_PLS S3_PLS NM_IO SLED ADCCH[0..3] /ADCSEL ADCHBEN /ADCSTART EXTSIG FL1SIG FL2 FL3 TXD0 RXD0 AUX1 AUX2 AUX3 /SRD /BSWR1 LASERMON RXD0 LASERMON BLR, GAIN & INTEGRATION EXTSIG FL1SIG FL2 FL3 YIRQ YIO XLED NM_ADC EXTINT FL1INT YTXD0 RXD0 TRCH[0..1] LASERMON /BSWR1 SA[0..2] SA[0..2] SD[0..7] SALE BRESET 20MHZ B S1_AWOFF S2_AWOFF S3_AWOFF BDINPUT[0..3] BDINPUT[0..3] SD[0..7] BSD[0..7] BSD[0..7] NM_INTG XIRQ YIRQ BLR, GAIN & INTEGRATION0 FL2 FL3 FL2INT FL2SIG FL3INT FL3SIG FL2 FL3 Extra circuits0 PG 5 XIO YIO HV2C HV3C /INTEGRATE /DUMP FL2INT FL2SIG FL3INT FL3SIG HV2C HV3C S2C S3C /BGAINSEL3 /BGAINSEL4 /BINTSEL3 /BINTSEL4 /BGAINSEL3 /BGAINSEL4 /BINTSEL3 /BINTSEL4 /BSWR2 S2C S3C AUX1 AUX2 AUX3 AUX1 AUX2 AUX3 PG 9 HV2_CTL HV3_CTL S2_PLS S3_PLS AUX1SIG AUX2SIG AUX3SIG HV2_CTL HV3_CTL S2_PLS S3_PLS AUX1SIG AUX2SIG AUX3SIG /BSWR2 A A NM_SPROC NM_XTRA BSD[0..7] BSD[0..7] DRAWN BY Union Biometrica Inc. DATE 35 Medford Street, Suite 101 Somerville, MA 02143 NM_INTG2 Title Sorter Controller - Module Interconnects DATE 8 7 6 5 4 3 Size C Document Number Date: 2 Tuesday, April 29, 2003 Rev 350-5001-SC0 Sheet D1 1 1 of 10 COPAS BIOSORT PAGE 1 8 7 6 5 4 3 2 1 C21, 0.47uF, removed from pin 2 of U16 AUX2SIG ADCCH3 ADCCH2 ADCCH1 ADCCH0 14 15 16 17 R11 ADCCH[0..3] - AD826 EXTSIG FL1SIG FL2SIG FL3SIG SD[0..7] U14 20 REF VDD GND 1 18 AGND +12V 12 27 VSS REF GND /ADCSEL /ADCSTART /SRD -12V ADCHBEN 24 23 1 4 2 22 3 GND ADG406 -5VA CS CONV RD HBEN BUSY 12/8 CLK SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 16 15 14 13 11 10 9 8 DB0 DB1 DB2 DB3 DB4 DB5 DB6 DB7 VIN 19 GND +5V TRCH[0..1] TRCH0 TRCH1 2 17 VDD FL3SIG FL2SIG FL1SIG EXTSIG 7 5 3 1 8 6 4 2 +12V + D THRESHSIG 7 6 13 VDD A0 A1 A2 U13B AD826 5 +12V 3 VSS -12V 14 GND GND EN -5VA 12 DGND GND P4 3 U18A LF444 2 C38 + +12V P4B 1 - C .33 11 100R 8 D R25 4.99K +5VA 21 VSS AD7876 7 6 5 4 3 2 1 S1 S2 S3 S4 S5 S6 S7 S8 ADG408 4 -12V RS106 1 16 15 GND J4 C 4 5 6 7 12 11 10 9 -12V EN 4.99K U19 range, neg. range ignored 1 2 A3 A2 A1 A0 18 U14: +/- 10 V FS input + 4 FL2INT FL3INT FL2SIG FL3SIG AUX1SIG U13A 3 8 LMSIG 28 1 AUX3SIG D 4 D P1B P2B P3B P4B S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 S15 S16 8 EXTINT FL1INT EXTSIG FL1SIG TP3 +12V U12 19 20 21 22 23 24 25 26 11 10 9 8 7 6 5 4 R23 100K CON7 GND +12V TP4 RS5 1 P1 P2 P3 P4 12 4.7K 13 C36 U18D LF444 4 P2 8 6 5 2 4 6 8 4 1 3 5 7 PRESS1 PRESS2 PRESS3 PRESS4 + P2B 14 P3 5 - 6 7 1.0K 2 R22 1.0K 3 + THRESHSIG + R21 THRESHOLD 7 /MCOATHRESH - P3B - R24 1.0K 1 4 C37 11 .33 +12V U18B LF444 U16 LF311 GND 11 .33 -12V 4 P1 10 B 9 C35 U18C LF444 +5V GND GND + 8 P1B B 11 .33 VR1 78L05 +12V IN OUT 1 +5VA + C92 10 .33 C90 IN OUT +12V 10 C97 .33 + 7 C39 4 VR4 79M12 C179 .33 .33 C23 C25 C27 C29 C176 .33 .33 .33 .33 .33 GND GND 2 IN -5VA C94 .33 2 -15V C98 C95 10 C100 .33 - IN OUT 3 -12V 10 A Union Biometrica inc. C99 .33 1 +3VREF 1 C93 .33 3 GND GND 8 3 1 OUT + -12V U15A LF412 + +12V 2 C175 .33 GND VR2 79L05 + C28 .33 -12V LMSIG GND .33 REF C26 .33 6 10.0K A C24 .33 GND 5 LASERMON C22 + 2 C91 .33 2 8 R12 C30 .33 U15B LF412 C96 +12V 3 GND GND 1 +15V GND -12V 3 VR3 LM341-12 35 Medford Street, Suite 101 Somerville, MA 02143 Title 4 Sorter Controller - ADC Subsystem -12V GND 8 7 6 GND 5 4 3 Size B Document Number Date: Tuesday, April 29, 2003 2 Rev 350-5001-SC0 Sheet D1 2 of 1 10 COPAS BIOSORT PAGE 2 8 7 6 5 4 3 2 1 R97 was 7.5K, R92 in parallel to C110 deleted, HV1: Grn; HV2: Yel; HV3: Red R83 Annotations corrected and added, 2.0K U71 pin 1 was +5 -15V SA[0..2] U71 4 R82 11 +3VREF 20 19 18 17 16 15 14 13 VREF DB0 DB1 DB2 DB3 DB4 DB5 DB6 DB7 VDD VSS GND 3 GND HV2C HV3C S2C S3C 1 GND 12 GND C72 R151 D 0 = 0mv 255 =1.20V ~4.7mV PER LEVEL +15V R85 +12V 10 STIR1 1 4.99K 2.0K 49.9R 4 SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 2 THRESHOLD 8 SD[0..7] 9 8 7 6 5 4 3 2 R84 6 U75B LF412 7 .33 4.99K 5 GND GND STIR2 0 = 0mv 255 = 1.20V ~4.7mV PER LEVEL + /DACLD VOUT1 VOUT2 VOUT3 VOUT4 VOUT5 VOUT6 VOUT7 VOUT8 - D A0 A1 A2 WR + 24 23 22 21 - SA0 SA1 SA2 U75A LF412 MX7228KN 8 J2 +5V C 3 4 5 6 7 8 9 10 1 2 22 BRESET VALVLD /VALVEN D0 D1 D2 D3 D4 D5 D6 D7 OUT1 OUT1 OUT2 OUT3 OUT4 OUT5 OUT6 OUT7 COM +24V CLR STROBE OE +24V C79 10 + UCN5801A GND U79 +5V STIR1 STIR2 HV1_CTL HV2_CTL HV3_CTL +24V C80 + BLAST+ BLAST- 10 UCN5801A S3C GND +5V - 0 = 0mv 255 = 2.99V ~11.7mV per level or ~4.3 V of HV per level LF412 .33 C GND -15V +5V R80 U74 RS103A 1 2 7 1 6 100K C155 + 2 1 C156 RS103B 100K Vin 4.99K Fout Iout Thrsh Iref 3 S1_PLS RS103C RC 5 6 C157 LM331 5 1 +5V 100K B +5V .001 R97 GND 3 CLR STROBE OE HV1_CTL 1 2 C110 4 +24V + GND 1 2 22 BRESET VALVLD2 /VALVEN OUT1 OUT1 OUT2 OUT3 OUT4 OUT5 OUT6 OUT7 COM U76A 3 VCC B D0 D1 D2 D3 D4 D5 D6 D7 VALVE9 VALVE10 VALVE11 VALVE12 VALVE13 VALVE14 VALVE15 VALVE16 20 19 18 17 16 15 14 13 12 4.99K 8 3 4 5 6 7 8 9 10 R91 4 SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 +15V 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 VALVE1 VALVE2 VALVE3 VALVE4 VALVE5 VALVE6 VALVE7 VALVE8 20 19 18 17 16 15 14 13 12 4 SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 8 U72 20.0K GND C158 .33 GND GND GND CON40 +24V TP9 GND 1 VALVE9 7 U73 IN OUT+ OUT- R75 A -15V V 2 /BLAST 6 BOOST THRESH SENSE +15V 5 4 3 A Union Biometrica Inc. G 1.5K +5V 1 R77 R76 UDN2962W R78 1.0 5.11K 6.19K +5V 8 GND .33 C78 .33 C113 C73 .33 .33 GND GND 7 +24V C71 GND 6 35 Medford Street, Suite 101 Somerville, MA 02143 GND 5 C112 .33 C77 .33 C111 .33 Title Sorter Controller - Valve & Motor Drivers C74 .33 GND 4 Size B Document Number Date: Tuesday, April 29, 2003 2 Rev 350-5001-SC0 D1 GND 3 Sheet 3 of 1 10 COPAS BIOSORT PAGE 3 8 7 6 5 4 3 2 1 RS3 +5V FL2 FL3 U4B 8 1 2 3 4 5 6 7 8 9 10 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 5 GND + D 7 D 6 - 4.7K LF412 4 J1 VDD TP1 7 3 15 14 +12V AD7524 AUX1 AUX2 AUX3 11 10 9 8 7 6 5 4 12 13 /BINTSEL1 /BSWR1 +12V C180 REF 16 RFB D0 D1 D2 D3 D4 D5 D6 D7 15pf OUT1 1 2 2 3 U1A R2 10.0K 1 OUT2 U5 8 D 6 LF412 GND CS WR VDD U4A 3 GND S + 1 3 - IN EXTINT LF412 -12V ADG417 GND 14 1 2 8 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 +12V GND 10.0K U3 2 5 R5 + GND 6 2.0K 4 /BGAINSEL1 /BSWR1 CS WR OUT2 15pf 1 R4 U1B LF412 8 12 13 OUT1 -12V 4 GND LASERMON PRESS1 PRESS2 PRESS3 PRESS4 D0 D1 D2 D3 D4 D5 D6 D7 C1 16 8 BSD4 BSD5 BSD6 BSD7 RFB + 1N5246B FL3 EXTSIG REF - D4 FL2 11 10 9 8 7 6 5 4 1 BSD0 BSD1 BSD2 BSD3 - 15 FL1 4 C U2 EXT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 C2 .01 +12V C U6 AD7524 8 /INTEGRATE +15V D 6 /DUMP 1 S IN ADG417 -15V U17B 8 CON26 5 GND + 7 6 - LF412 4 GND FL1SIG U7 15 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 VDD 3 GND 14 +12V +12V 11 10 9 8 7 6 5 4 AD7524 12 13 /BINTSEL2 /BSWR1 C181 REF RFB D0 D1 D2 D3 D4 D5 D6 D7 CS WR OUT1 OUT2 15pf 16 1 2 2 3 U9A R7 1 10.0K 8 D 6 S + 1 VDD 3 - FL1INT LF412 -12V ADG417 GND 14 B 1 2 IN LF412 GND U17A 3 GND U10 + 7 + CS WR GND TP2 +12V U8 - /BSWR1 5 10.0K 4 12 13 /BGAINSEL2 /BGAINSEL1 /BGAINSEL2 /BSWR1 /BINTSEL1 /BINTSEL2 2 R9 8 10 9 8 7 6 5 4 3 2 1 6 2.0K 4 RS2 OUT2 1 R8 U9B LF412 8 GND OUT1 -12V 1 1N5246B D0 D1 D2 D3 D4 D5 D6 D7 15pf 16 8 B 11 10 9 8 7 6 5 4 RFB 4 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 D5 C8 REF - 15 C9 .01 +12V U11 8 AD7524 /DUMP +5V D 6 S 1 IN /INTEGRATE 4.7K ADG417 BSD[0..7] A A Union Biometrica Inc. +12V VDD C3 .33 C5 .33 C7 .33 C11 .33 C13 .33 C15 .33 C17 .33 -12V C19 .33 VSS C4 .33 GND 35 Medford Street, Suite 101 Somerville, MA 02143 C6 .33 C10 .33 C12 .33 +5V VCC GND 8 7 6 5 4 3 Title Sorter Controller - Ext & Flu1 Chans Size B Document Number Date: Tuesday, April 29, 2003 2 Rev 350-5001-SC0 Sheet D1 4 of 1 10 COPAS BIOSORT PAGE 4 5 4 3 2 1 8 U104B 5 GND + 7 6 LF412 4 FL2SIG U102 6 2 5 U103 7 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 +12V GND 14 +12V D0 D1 D2 D3 D4 D5 D6 D7 12 13 /BINTSEL3 /BSWR2 FL3 16 RFB AD7524 +12V C182 REF 11 10 9 8 7 6 5 4 15pf OUT1 1 2 2 3 U101A R102 10.0K 1 OUT2 GND U105 8 D 6 LF412 GND CS WR VDD U104A 3 S + 1 3 FL2INT - LF412 IN -12V ADG417 GND 14 1 2 4 VDD 3 15 + GND TP101 + 1 10.0K 8 OUT2 R104 8 CS WR OUT1 2.0K 4 12 13 /BGAINSEL3 /BSWR2 D0 D1 D2 D3 D4 D5 D6 D7 15pf 8 GND D RFB R103 U101B LF412 1 D6 1N5246B 11 10 9 8 7 6 5 4 -12V 16 4 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 C120 REF - 15 FL2 - D C121 .01 +12V U106 AD7524 8 /INTEGRATE D 6 /DUMP 1 S IN C C ADG417 8 5 GND U117B + 7 6 LF412 4 FL3SIG U107 VDD 3 +12V GND 14 15 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 +12V RFB D0 D1 D2 D3 D4 D5 D6 D7 AD7524 12 13 /BINTSEL4 RS102 10 9 8 7 6 5 4 3 2 1 /BGAINSEL3 /BGAINSEL4 /BSWR2 C183 REF 11 10 9 8 7 6 5 4 CS WR /BSWR2 OUT1 OUT2 16 15pf 1 2 2 3 U109A R107 10.0K 1 GND U110 8 D + 7 + GND +12V U108 - 5 TP102 1 2 10.0K 6 S 1 + VDD 3 - FL3INT B LF412 -12V ADG417 GND 14 1 2 IN LF412 GND U117A 3 4 CS WR 6 R109 8 /BGAINSEL4 /BSWR2 OUT2 15pf 1 2.0K 4 12 13 OUT1 8 GND B RFB D0 D1 D2 D3 D4 D5 D6 D7 R108 U109B LF412 8 1N5246B 11 10 9 8 7 6 5 4 -12V 16 4 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 D7 C122 REF - 15 C123 .01 +12V U111 8 AD7524 /BINTSEL3 /BINTSEL4 6 /DUMP D S 1 IN ADG417 /INTEGRATE +5V 4.7K BSD[0..7] Union Biometrica Inc. A +12V VDD C131 .33 C133 .33 C135 .33 C137 .33 C139 .33 C141 .33 C143 .33 GND -12V C145 .33 C132 .33 C134 .33 C136 .33 C138 .33 +5V VCC GND 5 4 3 A 35 Medford Street, Suite 101 Somerville, MA 02143 VSS 2 Title Sorter Controller - Flu2 & Flu3 Chans Size B Document Number Date: Tuesday, April 29, 2003 Rev 350-5001-SC0 D1 Sheet 5 of 10 1 COPAS BIOSORT PAGE 5 8 7 6 5 4 3 2 1 XMC[0..2] RS4 H2 H3 H4 H5 H6 H7 H8 U51 XMC0 XMC1 XMC2 YMC0 YMC1 YMC2 YMC[0..2] D 2 3 4 5 6 7 8 9 S1_PLS S1_AWOFF A1 A2 A3 A4 A5 A6 A7 A8 1 19 Y1 Y2 Y3 Y4 Y5 Y6 Y7 Y8 X_PLS X_CW/CCW X_AWOFF Y_PLS Y_CW/CCW Y_AWOFF BS1_PLS BS1_AWOFF 18 17 16 15 14 13 12 11 H1 G1 G2 U56 10 9 8 7 6 5 4 3 2 1 XLED YLED SLED 2 3 4 5 6 7 8 9 H4 S2_PLS S2_AWOFF S3_PLS S3_AWOFF 1 19 4.7K +5V A1 A2 A3 A4 A5 A6 A7 A8 Y1 Y2 Y3 Y4 Y5 Y6 Y7 Y8 /XLED /YLED /SLED 18 17 16 15 14 13 12 11 R62 LED 330 D1 +5V BS2_PLS BS2_AWOFF BS3_PLS BS3_AWOFF R63 330 R64 330 D D2 +5V G1 G2 /SLED LED /XLED LED D3 +5V /YLED 74HC540 74HC540 GND GND XSW[0..1] YSW[0..1] U52 XSW0 XSW1 18 17 16 15 14 13 12 11 C YSW0 YSW1 BDINPUT0 BDINPUT1 BDINPUT2 BDINPUT3 Y1 Y2 Y3 Y4 Y5 Y6 Y7 Y8 RD1 A1 A2 A3 A4 A5 A6 A7 A8 G1 G2 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 1 19 C81 C83 C85 C87 .01 .01 BS1_PLS BS1_AWOFF BS2_PLS BS2_AWOFF BS3_PLS BS3_AWOFF DINPUT3 J5 1 2 3 4 .01 C84 C86 C88 .01 .01 .01 .01 10 9 8 7 6 5 4 3 2 1 C82 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 TXD +5V .01 GND +5V X_PLS GND CON4 X_CW/CCW X_AWOFF DINPUT1 RS1 4.7K GND B D8 1N5711 2 VALVE9 /XLOLIM /XHILIM /YLOLIM /YHILIM 1 Y_PLS U53 RXD 13 8 TXD 14 7 C55 1 C1+ C2V+ 11 10 TXD0 S1 1 GND 2 GND C58 4 3 GND 5 Y_CW/CCW Y_AWOFF DINPUT2 U55 PBRST ST TD RST TOL RST .33 2 7 SALE 5 DINPUT4 BRESET 6 +5V DS1232 TP5 TP6 TP7 TP8 1 V- C2+ RXD0 1 C1- T1IN T2IN 12 9 1 6 T1OUT T2OUT R1OUT R2OUT 1 3 .33 R1IN R2IN C J3 +5V RXD 470 74HC540 BDINPUT[0..3] /XLOLIM /XHILIM DINPUT1 /YLOLIM /YHILIM DINPUT2 DINPUT3 DINPUT4 16 15 14 13 12 11 10 9 B CON34 GND MAX202 C56 .33 A GND C57 .33 GND U54 OUT VCC 8 C51 .33 20MHZ OSC C52 .33 C53 .33 C54 .33 C101 .33 C102 .33 C59 100 + C60 7 6 GND GND A Union Biometrica Inc. + 35 Medford Street, Suite 101 Somerville, MA 02143 100 Title GND 8 GND +5V 20MHZ Sorter Controller - Misc I/O 5 4 3 Size B Document Number Date: Wednesday, September 11, 2002 2 Rev 350-5001-SC0 D1 Sheet 6 of 1 10 COPAS BIOSORT PAGE 6 8 7 6 5 4 3 2 1 SA[0..15] SD[0..7] SD[0..7] U61 35 GND D 21 20MHZ 20 10 BRESET TOF DAQIRQ SIRQ 14 15 16 17 2 3 4 5 6 7 8 9 S1_AWOFF S2_AWOFF S3_AWOFF BDINPUT0 BDINPUT1 BDINPUT2 BDINPUT3 SLED C EA/VP P0.0 P0.1 P0.2 P0.3 P0.4 P0.5 P0.6 P0.7 X1 X2 RESET P2.0 P2.1 P2.2 P2.3 P2.4 P2.5 P2.6 P2.7 P3.2/INT0 P3.3/INT1 P3.4/T0 P3.5/T1 P1.0/T2 P1.1/T2X P1.2/RXD1 P1.3/TXD1 P1.4 P1.5 P1.6 P1.7 RD WR PSEN ALE/P TXD0 RXD0 43 42 41 40 39 38 37 36 SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 24 25 26 27 28 29 30 31 SA8 SA9 SA10 SA11 SA12 SA13 SA14 SA15 19 18 32 33 13 11 /SRD /SWR /SPSEN SALE STXD0 U62 SD7 SD6 SD5 SD4 SD3 SD2 SD1 SD0 2 3 4 5 6 7 8 9 BSALE 11 1 D1 D2 D3 D4 D5 D6 D7 D8 U63 Q1 Q2 Q3 Q4 Q5 Q6 Q7 Q8 19 18 17 16 15 14 13 12 SA7 SA6 SA5 SA4 SA3 SA2 SA1 SA0 SA0 SA1 SA2 SA3 SA4 SA5 SA6 SA7 SA8 SA9 SA10 SA11 SA12 SA13 SA14 C OC 74HC573 R61 49.9R GND SA15 /SWR /SRD 10 9 8 7 6 5 4 3 25 24 21 23 2 26 1 20 27 22 DO D1 D2 D3 D4 D5 D6 D7 CE WR OE 11 12 13 15 16 17 18 19 SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 SA0 SA1 SA2 SA3 SA4 SA5 SA6 SA7 SA8 SA9 SA10 SA11 SA12 SA13 SA14 SA15 GND /SPSEN 20 22 A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 O0 O1 O2 O3 O4 O5 O6 O7 SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 18 17 16 15 14 13 12 11 BSD0 BSD1 BSD2 BSD3 BSD4 BSD5 BSD6 BSD7 CE OE 27C512 SALE C BSD[0..7] U67 SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 SA[0..15] U65 SA10 SA11 SA12 SA13 SA14 SA15 46 22 11 56 65 29 SD0 SD1 SD2 SD3 SD7 B 66 33 67 47 45 20MHZ 16 8 58 /SWR /SRD J6 GND ADCCH0 ADCCH1 ADCCH2 ADCCH3 SD0 SD1 SD2 SD3 SD7 BENABLE GAINSEL1 GAINSEL2 GAINSEL3 GAINSEL4 INTSEL1 INTSEL2 INTSEL3 INTSEL4 CLK SWR SRD 2 4 6 1 3 5 2 4 6 13 61 32 10 40 37 #PTHRSH NUDAT NIB3 NIB2 NIB1 NIB0 RS7 100R SIP6 3 12 5 6 ADCCH0 ADCCH1 ADCCH2 ADCCH3 62 57 28 25 23 64 30 24 26 /BENABLE /GAINSEL1 /GAINSEL2 /GAINSEL3 /GAINSEL4 /INTSEL1 /INTSEL2 /INTSEL3 /INTSEL4 74HC541 SA10 SA11 SA12 SA13 SA14 SA15 ADCCH[0..3] SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 /MCOATHRESH R65 49.9R /INTEGRATE R66 49.9R TRCH0 TRCH1 TRCH[0..1] 4 27 30 37 20 18 /SRD BDINPUT0 BDINPUT1 BDINPUT2 BDINPUT3 29 36 2 14 /SWR SA10 SA11 SA12 SA13 SA14 SA15 43 15 28 39 8 17 40 7 11 6 32 20MHZ /DUMP 43 44 Y1 Y2 Y3 Y4 Y5 Y6 Y7 Y8 G1 G2 /BENABLE U66 TOF DAQIRQ 31 4 38 42 TRCH0 TRCH1 A1 A2 A3 A4 A5 A6 A7 A8 1 19 /ADCSTART /ADCSEL ADCHBEN 14 TOF DAQIRQ INTEGRATE DUMP 2 3 4 5 6 7 8 9 CLK_1MHZ 7 27 41 48 CLK_1MHZ ADCSTART ADCSEL ADCHBEN SA10 SA11 SA12 SA13 SA14 SA15 THRESHOLD RS6 100R SIP6 1 3 5 C-GRID 8P SD0 SD1 SD2 SD3 SD4 SD5 SD6 SD7 CLK_1MHZ BLAST BOOST VALVLD VALVLD2 VALVEN DACLD DIN1 DIN2 DIN3 DIN4 ACQUIRE U68 25 44 26 38 5 41 /GAINSEL1 /GAINSEL2 /INTSEL1 /INTSEL2 /SWR /BLAST BOOST VALVLD VALVLD2 /VALVEN 2 3 4 5 6 7 8 9 /DACLD /BENABLE XTXD0 YTXDO STXD0 TXD0 CLK SWR SRD 21 SIRQ XIRQ YIRQ XIO YIO 16 9 24 XTXD0 YTXD0 A1 A2 A3 A4 A5 A6 A7 A8 1 19 Y1 Y2 Y3 Y4 Y5 Y6 Y7 Y8 18 17 16 15 14 13 12 11 /BGAINSEL1 /BGAINSEL2 /BINTSEL1 /BINTSEL2 /BSWR1 B G1 G2 74HC541 STXD0 U69 19 22 3 10 31 42 TXD0 SIRQ XIRQ YIRQ /GAINSEL3 /GAINSEL4 /INTSEL3 /INTSEL4 /SWR 2 3 4 5 6 7 8 9 /BENABLE 1 19 XIO YIO A1 A2 A3 A4 A5 A6 A7 A8 J6, RPx, and R161 can be pinswapped Y1 Y2 Y3 Y4 Y5 Y6 Y7 Y8 18 17 16 15 14 13 12 11 /BGAINSEL3 /BGAINSEL4 /BINTSEL3 /BINTSEL4 /BSWR2 G1 G2 SORT A 11 12 13 15 16 17 18 19 80C320PLCC BDINPUT[0..3] 1 2 3 4 5 6 7 8 10 9 8 7 6 5 4 3 25 24 21 23 2 26 27 1 CY7C199 RXD0 D U64 A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 74HC541 and restructured as required. A Union Biometrica Inc. SA[0..2] SA0 SA1 SA2 +5V C61 C62 C63 C64 C65 C66 C67 C68 C69 C70 C170 C171 C172 .33 .33 .33 .33 .33 .33 .33 .33 .33 .33 .33 .33 .33 35 Medford Street, Suite 101 Somerville, MA 02143 Title Sorter Controller - Sorter Processor /SRD /SRD GND 8 7 6 5 4 3 Size B Document Number Date: Tuesday, April 29, 2003 2 Rev D1 350-5001-SC0 Sheet 7 of 1 10 COPAS BIOSORT PAGE 7 8 7 6 5 4 3 2 1 D D XA[0..15] XD[0..7] U31 GND 35 21 20MHZ 20 C 10 BRESET 14 15 16 17 XIRQ XIO XMC[0..2] XMC0 XMC1 XMC2 XSW0 XSW1 XSW[0..1] H1 XLED 2 3 4 5 6 7 8 9 EA/VP X1 X2 U32 P0.0 P0.1 P0.2 P0.3 P0.4 P0.5 P0.6 P0.7 RESET P3.2/INT0 P3.3/INT1 P3.4/T0 P3.5/T1 P1.0/T2 P1.1/T2X P1.2/RXD1 P1.3/TXD1 P1.4 P1.5 P1.6 P1.7 P2.0 P2.1 P2.2 P2.3 P2.4 P2.5 P2.6 P2.7 RD WR PSEN ALE/P TXD0 RXD0 43 42 41 40 39 38 37 36 XD0 XD1 XD2 XD3 XD4 XD5 XD6 XD7 24 25 26 27 28 29 30 31 XA8 XA9 XA10 XA11 XA12 XA13 XA14 XA15 19 18 32 33 13 11 /XRD /XWR /XPSEN XALE XD7 XD6 XD5 XD4 XD3 XD2 XD1 XD0 2 3 4 5 6 7 8 9 BXALE U33 D1 D2 D3 D4 D5 D6 D7 D8 11 1 Q1 Q2 Q3 Q4 Q5 Q6 Q7 Q8 19 18 17 16 15 14 13 12 XA7 XA6 XA5 XA4 XA3 XA2 XA1 XA0 XA0 XA1 XA2 XA3 XA4 XA5 XA6 XA7 XA8 XA9 XA10 XA11 XA12 XA13 XA14 C OC 74HC573 GND R31 49.9R XA15 /XWR /XRD 10 9 8 7 6 5 4 3 25 24 21 23 2 26 1 20 27 22 A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 U34 DO D1 D2 D3 D4 D5 D6 D7 11 12 13 15 16 17 18 19 XD0 XD1 XD2 XD3 XD4 XD5 XD6 XD7 CE WR OE XA0 XA1 XA2 XA3 XA4 XA5 XA6 XA7 XA8 XA9 XA10 XA11 XA12 XA13 XA14 XA15 /XPSEN 10 9 8 7 6 5 4 3 25 24 21 23 2 26 27 1 20 22 GND CY7C199 A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 O0 O1 O2 O3 O4 O5 O6 O7 11 12 13 15 16 17 18 19 XD0 XD1 XD2 XD3 XD4 XD5 XD6 XD7 C CE OE 27C512 XTXD0 RXD0 80C320PLCC B B +5V C31 C32 C33 C34 .33 .33 .33 .33 GND A A Union Biometrica Inc. 35 Medford Street, Suite 101 Somerville, MA 02143 Title Sorter Controller - Stage X Driver 8 7 6 5 4 3 Size B Document Number Date: Tuesday, April 29, 2003 2 Rev 350-5001-SC0 Sheet D1 8 of 1 10 COPAS BIOSORT PAGE 8 5 4 3 2 1 R151 & R153 deleted at U150 inputs, Annotations corrected, R159 & R160 ref designations uncrossed, R157 and R160 were 7.5K Rev D: R159 & R160 again uncrossed. +15V U150A +5V D D 8 R150 4.99K HV2C LF412 3 + 1 HV2_CTL 2 C150 - 0 = 0mv 255 = 2.99 V ~11.7 mV per level or ~4.3 V of HV per level R158 4.99K RS104A U152 1 2 7 Vin 1 6 100K GND 1 C160 5 + 7 HV3_CTL 6 - LF412 S2_PLS RS104C RC 5 6 C161 LM331 5 +5V 100K 1 +5V .001 R157 GND 3 C151 0 = 0mv 255 = 2.99 V ~11.7 mV per level or ~4.3 V of HV per level Iref RS104B 100K 3 4 HV3C 2 + U150B 4 8 4.99K Fout Iout Thrsh GND C159 R152 VCC S2C -15V 8 4 .33 20.0K GND C162 .33 4 .33 GND GND C GND C GND +5V R160 4.99K LF444 7 - R155 2 Vin 1 6 AUX1SIG U151B C163 2 + C153 4 1 C164 Iref 3 S3_PLS RS105C RC 5 6 C165 LM331 5 +5V 100K 4 RS105B 100K .33 Fout Iout Thrsh 4 10.0K 7 100K GND + 5 AUX1 1 VCC S3C 11 6 U153 8 RS105A 1 .001 R159 +5V GND 3 GND B 20.0K B GND - 10 GND LF444 8 + R156 AUX2 C166 .33 GND 11 9 GND AUX2SIG U151C C154 10.0K 4 .33 GND +12V +15V -12V -15V -12V 11 C173 .33 C177 .33 C174 .33 C178 .33 Union Biometrica Inc. A LF444 1 - 2 R154 GND GND GND AUX3 A 35 Medford Street, Suite 101 Somerville, MA 02143 AUX3SIG + 3 GND U151A C152 10.0K Title 4 Sorter Controller - Misc Buffers & VFCs .33 GND 5 Size B Document Number Date: Tuesday, April 29, 2003 4 3 2 Rev 350-5001-SC0 +12V D1 Sheet 9 of 10 1 COPAS BIOSORT PAGE 9 8 7 6 5 4 3 2 1 YA[0..15] D D YD[0..7] U41 35 GND 21 20MHZ 20 10 BRESET 14 15 16 17 YIRQ YIO YMC[0..2] C YMC0 YMC1 YMC2 YSW0 YSW1 YSW[0..1] 2 3 4 5 6 7 8 9 GND YLED EA/VP X1 X2 U42 P0.0 P0.1 P0.2 P0.3 P0.4 P0.5 P0.6 P0.7 RESET P3.2/INT0 P3.3/INT1 P3.4/T0 P3.5/T1 P1.0/T2 P1.1/T2X P1.2/RXD1 P1.3/TXD1 P1.4 P1.5 P1.6 P1.7 P2.0 P2.1 P2.2 P2.3 P2.4 P2.5 P2.6 P2.7 RD WR PSEN ALE/P TXD0 RXD0 43 42 41 40 39 38 37 36 YD0 YD1 YD2 YD3 YD4 YD5 YD6 YD7 24 25 26 27 28 29 30 31 YA8 YA9 YA10 YA11 YA12 YA13 YA14 YA15 19 18 32 33 13 11 /YRD /YWR /YPSEN YALE YTXD RXD YD7 YD6 YD5 YD4 YD3 YD2 YD1 YD0 2 3 4 5 6 7 8 9 BYALE 11 1 U43 D1 D2 D3 D4 D5 D6 D7 D8 Q1 Q2 Q3 Q4 Q5 Q6 Q7 Q8 19 18 17 16 15 14 13 12 YA7 YA6 YA5 YA4 YA3 YA2 YA1 YA0 YA0 YA1 YA2 YA3 YA4 YA5 YA6 YA7 YA8 YA9 YA10 YA11 YA12 YA13 YA14 C OC 74HC573 GND R41 49.9R YA15 /YWR /YRD 10 9 8 7 6 5 4 3 25 24 21 23 2 26 1 20 27 22 A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 U44 DO D1 D2 D3 D4 D5 D6 D7 YD0 YD1 YD2 YD3 YD4 YD5 YD6 YD7 11 12 13 15 16 17 18 19 CE WR OE YA0 YA1 YA2 YA3 YA4 YA5 YA6 YA7 YA8 YA9 YA10 YA11 YA12 YA13 YA14 YA15 /YPSEN 10 9 8 7 6 5 4 3 25 24 21 23 2 26 27 1 20 22 GND CY7C199 A0 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 O0 O1 O2 O3 O4 O5 O6 O7 11 12 13 15 16 17 18 19 YD0 YD1 YD2 YD3 YD4 YD5 YD6 YD7 C CE OE 27C512 YTXD0 RXD0 80C320PLCC +5V B C41 C42 C43 C44 .33 .33 .33 .33 B GND A A Union Biometrica Inc. 35 Medford Street, Suite 101 Somerville, MA 02143 Title Sorter Controller - Stage Y Driver 8 7 6 5 4 3 Size B Document Number Date: Tuesday, April 29, 2003 2 Rev 350-5001-SC0 D1 Sheet 10 of 1 10 COPAS BIOSORT PAGE 10 8 7 6 4 3 9 18 26 8 17 25 7 16 24 6 15 23 5 14 22 4 13 21 3 12 20 2 11 19 1 10 FL3 FL1 FL2 FL2 PRESS3 PRESS4 HV3_CTL AUX1 AUX2 HV1_CTL HV2_CTL AUX3 XCTL2 XCTL2 XCTL1 +15V XCTL1 6 S2_HFS 3 +5V BS2_AWOFF 7 R5 5 +5V 2.0K 1 /S2_R 2 POT1 C3 220 PB PB Bsen /PB /PB DIR H/F* 11 12 8 9 10 S2_WA1 +5V PA PA Asen /PA /PA 19 20 21 17 18 S2_WB1 Iadj AUX2 6 S3_HFS 3 BS3_AWOFF 7 DINPUT3 R6 5 +5V SClk PB PB Bsen /PB /PB DIR H/F* 1 4.7K /S3_R 2 C10 +5V 220 DINPUT4 U2 +V S3_DIR +5V +V 4 11 12 8 9 10 +5V PA PA Asen /PA /PA Iadj 19 20 21 17 18 S3_WB1 S3_WB2 /XHILIM /YLOLIM 16 C12 + +5V .33 BS1_PLS BS1_AWOFF BS2_PLS BS2_AWOFF BS3_PLS BS3_AWOFF S1_DIR +5V DINPUT3 +5V +5V +5V J4 X_PLS X_PLS GND GND +24V J5 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Y_PLS Y_CW/CCW Y_AWOFF GND Y_BLUE Y_RED Y_GREEN Y_BLACK Y_WHITE Y_YELLOW GND +24V HDR16 J6 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 BS1_PLS S1_DIR BS1_AWOFF GND GND +24V S1_BLU S1_RED S1_GRN S1_BLK S1_WHT S1_YEL HDR16 X_BLUE X_RED X_GREEN X_BLACK X_WHITE X_YELLOW Y_BLUE Y_RED Y_GREEN Y_BLACK Y_WHITE Y_YELLOW GND TP12 GND 1 TP15 GND 1 TP16 4 TO STAGE GND 1 TP17 B J11 RXD TXD 1 2 3 GND TO BULKHEAD DB9 MTE X1 CONN A DRAWN BY Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 DATE Title DATE 5 1 14 2 15 3 16 4 17 5 18 6 19 7 20 8 21 9 22 10 23 11 24 12 25 13 GND CON34 6 TP11 1 C T1 CHECKED BY 7 1 DB25 HDR16 +5V 8 TP7 1 X_AWOFF DINPUT4 TP6 TP10 2 1 X_CW/CCW DINPUT2 1 1 J7 /XLOLIM /R 15 10 Y_CW/CCW Y_AWOFF TP5 GND 0V A is pulled up to +15V via 1.5K. X_BLUE X_RED X_GREEN X_BLACK X_WHITE X_YELLOW 1 TP9 PRESS3 TXD Y_PLS TP4 1 DB9 IB462H C11 /XLOLIM /XHILIM /YLOLIM /YHILIM TP3 1 TP8 PRESS1 PRESS4 .33 V16 becomes Optest, old XCTL2, which DINPUT1 TP2 1 MOTOR 3 S3_WA2 /YHILIM Existing Rework Not Shown: X_CW/CCW X_AWOFF 1 D 1 PRESS2 /EN 0V B TP1 1 AUX3 S3_WA1 500 RXD AUX2 5 9 4 8 3 7 2 6 1 DINPUT3 14 13 DINPUT2 POT2 S3_WA1 S3_WA2 S3_WB1 S3_WB2 .33 + C8 2.0K +5V FL3 AUX1 .33 +5V 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 MOTOR 2 16 15 C9 BS3_PLS JUMPER BLOCK 12 J3 1 J17 C7 + JP1 12 11 10 9 8 7 DINPUT2 DB9 +24V 1 2 3 4 5 6 +5V S2_WB2 FL2 IB462H 10 HD26 S2_DIR S2_HFS /S2_R S3_DIR S3_HFS /S3_R FL1 5 9 4 8 3 7 2 6 1 LASERMON .33 GND 10 9 8 7 6 5 4 3 2 1 S2_WA1 S2_WA2 S2_WB1 S2_WB2 500 C6 RS2 EXT /R CON26 C 1 REASON DATE J16 S2_WA2 /EN C5 APPROVAL U1 SClk 0V EXT 4 S2_DIR 0V /XSEL -15V BS2_PLS .33 +V +15V +V LASERMON PRESS1 PRESS2 +24V TO PMT/HV PCB C4 ECN NO. 14 -15V 13 FL1 FL3 2 REV + EXT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 D 5 J10 J1 3 Bulkhead Connector - Detectors & Steppers Size B Document Number Date: Tuesday, April 29, 2003 2 Rev 350-5002-SC0 A3 Sheet 1 of 1 2 COPAS BIOSORT BULKHEAD CONNECTOR PAGE 1 5 4 3 G2 G2 +24V VALVE15 G3 G3 VALVE16 G4 DINPUT1 DINPUT4 +15V +5V -15V AUX1 +5V AUX2 AUX3 STIR1 STIR2 AUXILLIARY S1_BLU S1_BLK S1_GRN S1_YEL S1_WHT +24V VALVE8 VALVE9 +24V DINPUT1 DINPUT2 AUX1 INTERLOCK +5V RS3 D VALVES & MOTOR 1 DB25 M8 CON1 C /XSEL 10 9 8 7 6 5 4 3 2 1 BLAST+ BLAST- J8 LASERSET 1 9 2 10 3 11 4 12 5 13 6 14 7 15 8 JP3 1 2 3 4 8 7 6 5 VALVE10 S1_DIR VALVE11 VALVE12 +15V +24V LASERMON GND LASERSET B +5V R2 1.30K R3 2.21K R4 1.30K C2 + R1 332 C1 D1 LED D2 LED VALVE1 VALVE2 VALVE3 VALVE4 VALVE6 VALVE7 J9 INTERLOCK +5V JUMPER BLOCK 8 D3 LED TO LASER ASSEMBLY GND GND PRESS1 PRESS2 PRESS3 PRESS4 B DB15 DB15 10 GND +24V -15V +5V +15V GND GND GND GND .33 GND J15 1 2 3 4 5 6 7 8 9 10 11 12 CON12 RS1 4.7K +5V J13 1 2 3 4 AUX POWER CON4 GND DB9 SW1 1 2 3 4 5 6 S1A S2A S3A S4A S5A S6A S1B S2B S3B S4B S5B S6B 12 11 10 9 8 7 +5V VALVE8 VALVE9 VALVE10 VALVE11 VALVE12 VALVE13 VALVE14 VALVE15 VALVE16 DIP SWITCH 4 5 9 4 8 3 7 2 6 1 +24V +5V GND CON6 5 TO VALVE ASSEMBLY 10 9 8 7 6 5 4 3 2 1 J12 +24V D4 LED -15V 1 9 2 10 3 11 4 12 5 13 6 14 7 15 8 +5V LS1 1 2 3 4 5 6 M7 CON1 +24V GND A M6 CON1 is pulled up to +15V via 1.5K. S1_DIR +5V GND M5 CON1 V16 becomes Optest, old XCTL2, which 4.7K CON40 +5V M4 CON1 Existing Rework Not Shown: DB25 HV1_CTL HV2_CTL HV3_CTL M3 CON1 1 DINPUT3 VALVE5 +24V S1_RED M2 CON1 1 +24V VALVE9 VALVE10 VALVE11 VALVE12 VALVE13 VALVE14 VALVE15 VALVE16 DINPUT2 JUMPER BLOCK 12 GND M1 CON1 1 +24V VALVE14 1 14 2 15 3 16 4 17 5 18 6 19 7 20 8 21 9 22 10 23 11 24 12 25 13 1 VALVE1 VALVE2 VALVE3 VALVE4 VALVE5 VALVE6 VALVE7 VALVE8 G4 J14 BLAST+ BLASTSTIR1 STIR2 13 25 12 24 11 23 10 22 9 21 8 20 7 19 6 18 5 17 4 16 3 15 2 14 1 G1 12 11 10 9 8 7 1 C G1 1 2 3 4 5 6 1 D VALVE13 JP2 +5V 1 J18 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 1 1 J2 2 3 XCTL1 XCTL2 XCTL1 XCTL2 A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title Bulkhead Connector - Valves & Red Laser 2 Size B Document Number Date: Tuesday, April 29, 2003 Rev 350-5002-SC0 A3 Sheet 2 of 2 1 COPAS BIOSORT BULKHEAD CONNECTOR PAGE 2 8 7 6 5 4 3 2 1 REV ECN NO. APPROVAL DATE C7 U4 1 33pf R4 100K 7 D 8 C8 -V JP1 B1 W1 A1 CS SDI CLK VDD GND 3 4 5 XCTL2 XCTL1 6 2 J2 JP2 AD8400AR1 JUMPER U1 5 8 4 4 R3 2 3 + 49.9 - R2 6 8 AD829 1 1 7 1 +V 3 -V 6 R7 +V SD103 12 R6 1 2 - 4 + 3 OUT+ DIN- OUT- V+ VOCM 4 R14 4.99K 5 8 1 2 V- R15 4.99K AD8131 U2A LT1457S8 + R5 - 10K .22 U2B 2 C 10K 100K -V R1 C1 DIN+ D1 2 Hamamatsu S7510 C2 +V U5 33pf D2 3.57K D SMB JACK +V 7 C J1 5 JP3 R8 49.9 R9 49.9 1 2 3 4 5 6 7 8 9 10 11 12 100K 6 LT1457S8 +15V JUMPER 6 D3 +V +V +V 1 -15V XCTL2 2 R10 XCTL1 1K LED R12 4.99K R13 CON12 4.99K B B +V HOLE7 MH95 +V VR1 C3 C5 C9 C15 .22 .22 .22 .22 + C13 22 8 +15V 2 3 6 7 C11 .22 Vin Vout SHIELD 1 +V GND GND GND GND -V JP1, JP2 & JP3 are installed, U4 & U5 not installed at 78L12-sm .22 C6 .22 C10 .22 + C4 22 A current revision. VR2 C14 2 3 6 7 -15V C12 5 .22 Vin Vin Vin Vin Vout 1 -V A Union Biometrica Inc. DRAWN BY GND 35 Medford Street, Suite 101 Somerville, MA 02143 79L12-sm DATE Title Extinction Detector & Preamplfier CHECKED BY DATE 8 7 6 5 4 3 Size B Document Number Date: Tuesday, April 29, 2003 2 Rev 350-5008-SC0 A3 Sheet 1 of 1 1 COPAS BIOSORT EXTINCTION DETECTOR 5 4 3 2 High Voltage 1 HV1_CTL D HV1_CTL 1 High Voltage 0 HV1 HV1 TO BULKHEAD CONNECTOR C -15V PMT_HV1 PMT_Preamp1 High Voltage 2 High Voltage 3 D FL1- FL1- FL3FL3+ FL2FL2+ FL1FL1+ +15V +15V_HV HV3_CTL HV1_CTL HV2_CTL HV2_CTL HV2_CTL HV2 HV2 FL2+ FL2+ XCTL2 FL2- FL2- XCTL1 C +15V /XCTLSEL EXTEXT+ 1 9 18 26 8 17 25 7 16 24 6 15 23 5 14 22 4 13 21 3 12 20 2 11 19 1 10 FL1+ FL1+ P1 D4 PMT_HV2 PMT_Preamp2 High Voltage 4 High Voltage 5 LED HD26 2 1 D5 LED HV3_CTL HV3_CTL HV3 HV3 FL3+ FL3+ FL3- 2 FL3- J1 DETECTOR B D6 -15V LED PMT_Preamp3 PMT_HV3 +15V 2 TO EXTINCTION 1 15 10 5 14 9 4 13 8 3 12 7 2 11 6 1 B R102 XCTL2 XCTL1 732 /XCTLSEL EXT+ EXT- PMT Test HDB15 XCTL1 XCTL2 XCTL1 XCTL2 A A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title PMT_Test PMT Preamp/HV - Top Level 5 4 3 2 Size B Document Number Date: Tuesday, April 29, 2003 Rev A1 350-5009-SC0 Sheet 1 of 1 1 COPAS BIOSORT PMT PAGE 1 5 4 3 2 1 D D HVPS1 VR1 3 Vout Vout Vin ADVANCE GM121K5NS HV1 = HV1_CTL*R9*1000/R15 1 Vin Vout 2 HV1 Adj +15V_HV 2 4 C2 22 + Gnd R2 Gnd LM317EMP 1 C1 .22 243 4 3 +15V_HV 4 - 10K E C R6 2 + 7 - 26.1K R3 U1B LF444CM 5 R7 6 8 U1C LF444CM + 11 - 2K 100M U1D LF444CM 4 2N3904 1 3 4 B + U1A LF444CM 4 R5 C R4 1.74K Q1 10 R9 9 12 + 14 C 13 11 - 732 R8 10K 11 R10 11 -15V C3 100K R11 R12 26.1K R13 10K 2K 1 +15V_HV -15V C4 .01 R15 C65 .22 C66 .22 2K HV1_CTL B B A A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title PMT Preamp/HV - HV1 5 4 3 2 Size B Document Number Date: Wednesday, April 30, 2003 Rev 350-5009-SC0 Sheet A1 2 of 7 1 COPAS BIOSORT PMT PAGE 2 5 4 3 HVPS2 VR2 D 3 Vin 1 ADVANCE GM121K5NS D 1 Vin Vout 2 HV2 Adj +15V_HV 2 4 Vout Vout 2 + C6 22 Gnd R16 Gnd LM317EMP 1 C5 .22 HV2 = 243 4 3 HV2_CTL*R23*1000/R29 +15V_HV 4 R19 - 10K 3 R20 2 + 7 E - 26.1K R17 U2B LF444CM 5 R21 6 8 U2C LF444CM + 11 - 2K 100M U2D LF444CM 4 2N3904 + 1 4 B U2A LF444CM 4 Q2 C R18 1.74K 10 R23 9 12 + 14 13 11 - 732 10K 11 R24 11 -15V 100K R25 C7 C R26 R22 26.1K C R27 10K 2K 1 C8 .01 R29 2K +15V_HV -15V HV2_CTL C67 .22 B C68 .22 B A A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title PMT Preamp/HV - HV2 5 4 3 2 Size B Document Number Date: Wednesday, April 30, 2003 Rev 350-5009-SC0 A1 Sheet 3 of 7 1 COPAS BIOSORT PMT PAGE 3 5 4 3 2 1 D D HVPS3 VR3 3 Vout Vout Vin 1 ADVANCE GM121K5NS Vin Vout 2 HV3 Adj +15V_HV 2 4 + C10 22 Gnd R30 Gnd LM317EMP 1 C9 .22 HV3 = HV3_CTL*R37*1000/R43 243 4 3 +15V_HV 4 - 10K R34 2 + 7 E - 26.1K R31 U3B LF444CM 5 R35 6 8 U3C LF444CM + 11 - 2K 100M U3D LF444CM 4 2N3904 1 3 4 B + U3A LF444CM 4 C R33 C R32 1.74K Q3 10 R37 9 12 + 14 13 11 - 732 C R36 10K 11 R38 11 -15V C11 100K R39 R40 26.1K R41 10K 2K 1 C12 .01 R43 2K B B +15V_HV -15V C69 .22 HV3_CTL C70 .22 A A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title PMT Preamp/HV - HV3 5 4 3 2 Size B Document Number Date: Wednesday, April 30, 2003 Rev 350-5009-SC0 A1 Sheet 4 of 7 1 COPAS BIOSORT PMT PAGE 4 5 4 3 2 1 J2 SMB JACK 1 C13 10pf 2 3 D D R44 100K JP1 C14 -V1 JUMPER U4 5 8 4 2 3 R47 8 AD829 1 1 7 3.57K 1 +V1 3 -V1 6 R49 +V1 1N5711 DY9 C15 OUT+ DIN- OUT- V+ VOCM R46 4.99K 5 R51 1 10K 100K 2 V- R50 4.99K R53 332K C17 .01 -V1 DY8 2 R48 4 + 3 5 C18 .01 DY7 U6A LT1457S8 C 8 R103 49.9 FL1+ 1 100K FL16 LT1457S8 PMT AD8131 7 + 332K U6B - R54 2 C DY9 DY8 DY7 DY6 DY5 DY4 DY3 DY2 DY1 C16 .01 - 11 10 9 8 7 6 5 4 3 2 332K DIN+ 4 D1 2 R52 PMT1 +V1 U5 68pf 6 + 1K - R45 R104 49.9 6 1 R55 +V1 332K JP2 DY6 JUMPER R56 332K DY5 +V1 R57 332K VR4 DY4 C19 + C20 .22 R58 C21 .22 C22 .22 2 3 6 7 22 C23 332K B 8 +15V DY3 .22 R59 Vin Vout 1 +V1 GND GND GND GND B 78L12-sm 332K DY2 VR5 2 3 6 7 -15V R60 332K DY1 -V1 C24 5 R61 .22 Vout 1 -V1 GND 79L12-sm 332K C26 .22 HV1 C27 .22 C28 .22 + K Vin Vin Vin Vin C25 22 A A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title PMT Preamp/HV - Flu1 PMT 5 4 3 2 Size B Document Number Date: Wednesday, April 30, 2003 Rev 350-5009-SC0 A1 Sheet 5 of 7 1 COPAS BIOSORT PMT PAGE 5 5 4 3 2 1 J3 SMB JACK 1 C29 10pf D D 2 3 R62 100K JP3 C30 -V2 JUMPER U7 5 8 4 R65 1 +V2 3 -V2 6 R67 1N5711 1 R69 -V2 2 U9B R66 - 4 + 3 DY7 OUT- V+ VOCM 4 R64 4.99K 5 8 1 2 V- R68 4.99K C 7 R105 5 49.9 FL2+ 100K FL2- 6 LT1457S8 PMT OUT+ DIN- AD8131 U9A LT1457S8 + C34 .01 10K 100K C33 .01 2 332K DIN+ D2 +V2 DY8 R72 1 2 C31 332K 8 C32 .01 DY9 R71 6 AD829 - 11 DY9 DY8 DY7 DY6 DY5 DY4 DY3 DY2 DY1 10 9 8 7 6 5 4 3 2 C 332K 3 +V2 U8 68pf 1 7 3.57K R70 PMT2 2 + 1K - R63 6 1 R73 R106 49.9 +V2 332K DY6 JP4 JUMPER R74 332K DY5 R75 +V2 VR6 332K 8 +15V DY4 B C35 .22 R76 C36 .22 C37 .22 + C38 22 2 3 6 7 C39 .22 332K DY3 Vin Vout 1 +V2 GND GND GND GND B 78L12-sm R77 332K VR7 DY2 C40 2 3 6 7 .22 5 -15V R78 332K DY1 -V2 R79 Vin Vin Vin Vin Vout 1 -V2 GND 79L12-sm 332K C41 .22 HV2 C42 .22 C43 .22 + K C44 22 A A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title PMT Preamp/HV - Flu2 PMT 5 4 3 2 Size B Document Number Date: Wednesday, April 30, 2003 Rev 350-5009-SC0 A1 Sheet 6 of 7 1 COPAS BIOSORT PMT PAGE 6 5 4 3 2 1 J4 SMB JACK 1 C45 10pf 2 3 D D R80 100K JP5 C46 -V3 JUMPER U10 5 8 4 1K 2 - 3 R83 8 AD829 1 1 7 3.57K C48 .01 1 +V3 3 -V3 6 R85 +V3 1N5711 DY9 C47 OUT+ DIN- OUT- V+ VOCM R82 4.99K 5 R87 1 10K 100K 2 V- R86 4.99K R89 332K C49 .01 -V3 2 R84 4 + 3 U12A LT1457S8 C 8 R107 5 C50 .01 DY7 49.9 1 FL3+ 100K FL3- 6 LT1457S8 PMT AD8131 7 + 332K U12B - R90 2 DY8 - 11 DY9 DY8 DY7 DY6 DY5 DY4 DY3 DY2 DY1 10 9 8 7 6 5 4 3 2 C 332K DIN+ 4 D3 2 R88 PMT3 +V3 U11 68pf 6 + R81 6 1 R91 R108 49.9 +V3 332K DY6 JP6 JUMPER R92 332K DY5 R93 +V3 332K VR8 DY4 R94 + C52 .22 332K B 8 +15V C53 .22 C54 .22 C51 22 2 3 6 7 C55 DY3 .22 R95 Vin Vout 1 +V3 GND GND GND GND B 78L12-sm 332K DY2 VR9 2 3 6 7 -15V R96 332K DY1 C56 5 R97 -V3 .22 Vout 1 -V3 GND 79L12-sm 332K HV3 C58 .22 C59 .22 C60 .22 + K Vin Vin Vin Vin C57 22 A A Union Biometrica Inc. 35 Medford Street Somerville, MA 02143 Title PMT Preamp/HV - Flu3 PMT 5 4 3 2 Size B Document Number Date: Tuesday, April 29, 2003 Rev 350-5009-SC0 A1 Sheet 7 of 7 1 COPAS BIOSORT PMT PAGE 7 Contact Information Union Biometrica, Inc. has offices in the United States and Europe. US - Global Headquarters Union Biometrica, Inc. 35 Medford Street, Suite 101 Somerville, Massachusetts 02143 Tel: +617-591-1211 Fax: +617-591-8388 Email: [email protected] Europe Union Biometrica N.V. European Scientific Operations Cipalstraat 3 B-2440 Geel, BELGIUM Tel: +32 (0)14 570 620 Fax: +32 (0) 14 570 621 Email: [email protected] COPAS™ is a registered trade mark of Union Biometrica, Inc. Twister™ is a registered trade mark of Zymark Corporation. Union Biometrica, Inc. Confidential www.unionbio.com 34 Notes Union Biometrica, Inc. Confidential www.unionbio.com 35 Union Biometrica, Inc. Confidential www.unionbio.com 36