Download NimbleGen Arrays User's Guide: Sequence Capture Array

Appendix B. Sequencing with Next Generation Platforms ....................75
Recommended Procedure: 454 GS FLX Titanium Series Sequencing ......... 75
Reference.......................................................................................................... 75
Process: GS FLX Titanium Series ................................................................... 76
Appendix C. Sequence Capture with Multi-Array Sets ..........................79
Instructions for Pooling Library Preparations for Hybridization
to Multi-Array Sets........................................................................................ 80
Appendix D. Sequence Capture with 2.1M Arrays..................................83
Differences between 385K and 2.1M Sequence Capture Experiments......... 83
Appendix E. Alternative Elution Method Using Elution Reagent ..........85
Step 1. Prepare for Wash and Elution ............................................................ 85
Step 2. Disassemble and Wash the Microarrays ............................................ 85
Step 3. Elute the DNA ..................................................................................... 85
Appendix F. Recommendations for High-Throughput Sequence
Capture Experiments ...................................................................................89
Appendix G. Limited Warranty.....................................................................91
NimbleGen Arrays User’s Guide: Sequence Capture Array Delivery v3.1
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