Download A Practical User's Guide

CONTENTS
Preface
xi
1 Introduction to LC/MS
1.1
1.2
1.3
1.4
1.5
Why LC/MS?, 1
Molecular Weights and Structure Studies, 4
LC/MS Systems, 4
System Costs, 7
Competitive Systems, 7
2 The HPLC System
2.1
2.2
2.3
2.4
9
HPLC System Components, 9
Gradient versus Isocratic Systems, 14
Micro HPLC Systems, 16
HPLC Tubing and Fittings, 18
3 The HPLC Column and Separation Modes
3.1
3.2
3.3
3.4
3.5
3.6
3.7
1
21
Column Construction, 21
Column Packing Materials, 23
Normal-Phase Columns, 25
Other Bonded-Phase Silica Columns, 26
Optimizing Reverse-Phase Column Use, 28
Silica Ion-Exchange Columns, 30
Silica Size-Separation Columns, 31
vii