Download Unibrain 830 User manual

Using a FLIM ready Radiance MP
Type in some useful information into the Author, Company or Contents fields, and click on ‘Save’ so save the file.
The file contains the complete data set, i.e. the decay curves with the number of points defined by ‘ADC Resolution’ in all
pixels of the FLIM image. Furthermore, the complete setup parameter set is saved with the data. You can load the data
file later to run a measurement with identical setup parameters.
5.12
Loading a FLIM Image
The SPC data files do not only contain the data but also the complete data set, i.e. the decay curves with the number of
points defined by ‘ADC Resolution’ in all pixels of the FLIM image. You can load an SPC data file to run a measurement
with parameters identical to that of an earlier measurement.
To load the data and the setup, open the ‘Load’ panel, and select the option ‘SPC data’. Select the right file and click on
‘Load’.
Loading FLIM and system setup data
5.13
FLIM Data Analysis
The data sets saved by the SPCM software contain the fluorescence decay curves in the individual pixels. To convert
these data into colour-coded lifetime images the bh ‘SPCImage’ software is used, see figure below.
9MRC60UM11
Issue 1
Page 23