Download MetaMorph NX User Guide

MetaMorph® NX Microscopy Automation & Image Analysis Software User Guide
8. To use auto expose for the channel, select the Auto Expose check box and follow Steps 911. If you are not using auto expose, clear the check box and proceed to Step 12.
Note: If your sample is light sensitive, you may want to minimize the number of auto
exposures.
9. In the Target Intensity field, type the maximum intensity value that auto exposure should
attempt to attain for the brightest pixel in the image. The default target intensity value is 75% of
the maximum intensity value that the camera driver is able to obtain. The target intensity will
determine the exposure time during auto expose.
10. In the Initial Exposure field, type the length of the first exposure attempt. Type an exposure
time that is well below the typical exposure time required for this type of experiment; generally,
the initial exposure time should be approximately one-tenth of the typical exposure time. If you
do not know the required exposure time, type 1 or 5 (or use the default value).
11. In the Maximum Exposure field, type the longest exposure time that you want to allow. Be
careful not to over expose your sample, which may result in bleaching, photo damage, or photo
toxicity.
12. In the Z Offset field, specify the difference between the focus position of the Z motor in this
channel and the focus position of the Z motor in the first channel. If the acquisition includes a Z
series, the entire Z series will be shifted by the Z offset distance.
Note: The Z Offset field is not available for the first channel that you define in the
Channels section.
In the example below, the position of the Z motor when the sample is in focus using Channel 1
is 500. The position of the Z motor when the sample is in focus using Channel 2 is 700. The
difference between the two positions is 200, so the Z offset distance is 200.
The next steps that you take depend on whether you want to use the displayed value of a camera
setting for all channels or if you want to set a specific camera setting for each channel. For example,
you may want to use the same shutter state for all channels but use a different gain for each channel.
If you are using just one channel for your acquisition, you can leave all of the check boxes cleared and
type or select the values in this list (in this case there is no need to set the values for each channel).
13. Click Camera Settings to expand the list of camera settings. The units of measurement that
are shown for each camera setting reflect the range that the camera supports.
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