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HBO 100 Microscope Illuminating System Operating Manual Microscope Illuminating System HBO 100 Copyright Carl Zeiss Knowledge of this manual is required for the operation of the instruments. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change. © Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model. Published by: Carl Zeiss MicroImaging GmbH P.O.B. 4041, 37030 Göttingen, Germany Phone: +49 (0) 551 5060 660 Fax: +49 (0) 551 5060 464 E-mail: [email protected] www.zeiss.de Number of this manual: 46-0063 e Date of issue: 03/01/2006 B 46-0063 e 03/06 1 Carl Zeiss Contents Microscope Illuminating System HBO 100 Contents 1 Notes on Safety ......................................................................................4 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 General ................................................................................................................ 4 Regulations concerning Instrument Safety and Electromagnetic Compatibility (EMC) ................................................................................................................... 4 Notes on Unpacking, Transport and Storage ......................................................... 5 Hints on Use ......................................................................................................... 5 Hints on Maintenance and Care............................................................................ 6 Advice on lamp breakage...................................................................................... 6 Hints on Disposal .................................................................................................. 7 Hints on Warranty................................................................................................. 7 2 Instrument Description ..........................................................................8 2.1 2.2 Components of the Microscope Illuminating System ............................................ 8 Technical Data of the Microscope Illuminating Systems ......................................... 9 3 Start-Up .................................................................................................11 3.1 3.2 3.3 3.4 Lamp Insertion and Change ................................................................................ 11 Start-Up of HBO 100 Microscope Illuminating System ......................................... 13 Focusing and Centering the HBO 100 Microscope Illuminator (423010) ............... 14 Focusing and Centering the HBO 100 Microscope Illuminator, self-adjusting (423011-9901) ..................................................................................................... 15 Setting Offset Values on HBO 100 Microscope Illuminator, self-adjusting (423011-9901) ................................................................................................... 16 Inserting the Collector Attachment ..................................................................... 17 3.5 3.6 2 B 46-0063 e 03/06 Microscope Illuminating System HBO 100 Illustrations Carl Zeiss Illustrations Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig. 10 Fig. 11 B 46-0063 e 03/06 HBO 100 Microscope Illuminating System (manual adjustment) ............................ 8 HBO 100 Microscope Illuminating System, self-adjusting...................................... 8 Line spectrum of the HBO 100/103 mercury short-arc lamps............................... 10 Inserting the HBO 103 lamp ............................................................................... 11 Rear of transformer for HBO 100 ........................................................................ 13 Front of transformer for HBO 100....................................................................... 13 Lamp arc and mirror image................................................................................. 14 Adjusting elements of HBO 100 for manual adjustment...................................... 14 Adjustment of HBO 100, self-adjusting ............................................................... 15 Keys for setting offset values for the adjustment of lamp arc and mirror image... 16 Inserting the collector attachment ...................................................................... 17 3 Carl Zeiss 1 Notes on Safety 1.1 General Safety General, EMC Please make yourself familiar with the contents of these operating instructions before attaching and starting up the HBO 100 Microscope Illuminating System. Additional information is available from our maintenance service or from authorized agencies. The HBO 100 Microscope Illuminating System should be used with Zeiss microscopes exclusively. The HBO 100 Microscope Illuminating System may only be handled by trained personnel. To ensure that the illuminating system functions properly with the microscope, the appropriate microscope manual must be observed. In addition, observe the manual "HBO 100 – Electronic ballast“. Special regard must be paid to the notes on instrument safety included in this manual. The data sheets from the lamp manufacturers must be observed when the HBO 103 lamps are operated. To guarantee the safe operation and function of the HBO 100 Microscope Illuminating System, it is necessary under any circumstances to take the precautions and observe the warnings contained in the operating instructions. The following warning and information symbols are used in this manual: 4 Microscope Illuminating System HBO 100 CAUTION! Non-observance of the safety notes constitutes a hazard for the user. CAUTION! Dangerous electrical voltage. CAUTION! Disconnect the instrument from the line before opening it! CAUTION! Hot surface. CAUTION! Non-observance of the safety notes constitutes the risk of damage to the instrument. NOTE! Notes which must be observed when using the HBO 100 Microscope Illuminating System. 1.2 Regulations concerning Instrument Safety and Electromagnetic Compatibility (EMC) The HBO 100 Microscope Illuminating System has been designed, produced and tested in compliance with EN 61010, Part 1 (VDE 0411). It was delivered in a technically safe and perfect state. The illuminating system meets the requirements of EC directives 73/23 and 89/336 and the EMC legislation, as amended on September 18, 1998. Radio-noise suppression complies with EN 55011, Class B, noise immunity with EN 61326. Compliance with the EC directives mentioned is documented by the CE certificate. B 46-0063 e 03/06 Microscope Illuminating System HBO 100 1.3 Safety Unpacking, Use Notes on Unpacking, Transport and Storage 1.4 Carl Zeiss Hints on Use The lamps may be removed from the package only if the protection mask (Cat. No. 417009) and the safety gloves with artery protection (Cat. No. 417008) are worn. − When they are hot, the lamps are under high internal pressure. They may only be inserted in the microscope illuminator if a safety mask and safety gloves (see chapter 1.3) are worn. For safety reasons, a safety holder is inserted in the microscope illuminator instead of the bulb during transport. Both the HBO 100 microscope illuminator and the HBO 103 lamp must be transported in their original packaging. This packaging must be kept for storage and disposal. − The HBO 103 lamps are under high pressure during operation; they must always be operated in closed lamp housings! The transport and storage temperatures to be observed correspond to those of the microscope stands. − The light of the HBO 103 short-arc lamps contains a high amount of UV light: Î Never look directly into the lamp or in reflected light Î Wear safety glasses (sunglasses) when operating the lamps Î Cover inspection openings with filters − The UV radiation of the lamps produces little quantities of harmful ozone. Take care to ventilate the working room with fresh air. − Both the HBO 100 microscope illuminator and the HBO 103 lamps must be protected from impact and humidity. Always keep the ventilation slats clear and ensure that no objects enter the instrument through the ventilation slats. − If the lamp bulb has broken, observe the information provided by the lamp manufacturer. Instead of the HBO 103 lamp, its predecessor HBO 100 can also be used. B 46-0063 e 03/06 5 Carl Zeiss 1.5 Safety Microscope Illuminating System Maintenance, Care, Lamp breakage HBO 100 Hints on Maintenance and Care To ensure perfect function of the microscope illuminating system, it is necessary to use only components and spares marketed or approved by us. When in doubt, please contact our service staff. With the exception of the work described in this manual, the user must not perform any repairs or changes himself. We would like to emphasize that all other changes and repairs must be performed by specially authorized personnel. Defective instruments or components may only be repaired and maintained by our service department. − After expiry of the average operating time (see data sheets from the lamp manufacturers), the lamps must be exchanged to avoid the risk of explosion! The operating time of the HBO 103 can be checked on the display of operating hour meter of the relevant transformer. (Fig. 6) With increasing service life, dirt will deposit on the lamp bulb so that homogeneous illumination of the object field is no longer ensured. − The lamps must be exchanged as described in chapter 3.1. − Before each lamp exchange, make sure to allow the HBO 100microscope illuminator to cool down sufficiently long in order to reduce the pressure; wait at least for 15 minutes! − Clean the housing of the microscope illuminator when it is dirty. Do not use any solvents. Soak a cleaning cloth in a mixture of water and washing-up liquid, remove the dirt and dry the housing using a clean cloth. 1.6 Advice on lamp breakage If the HBO lamps have reached their rated lifetime or their bulbs show advanced blackening, they should be replaced because of the increasing risk of explosion. If lamps are operated properly, lamp explosion is very unlikely. For the rare case, that an HBO lamp bursts and the mercury (Hg) contained in the bulb is released, we recommend to follow this procedure: − First of all, the persons being nearby should instantly leave the place to avoid inhaling mercury vapor. − Air the laboratory thoroughly (for approx. 30 minutes)! − Every lamp housing of a higher-power lamp should be equipped with an individual exhaust device that is not connected to the domestic air-conditioning system to avoid that mercury vapor enters this system. − Once the lamp housing has cooled down, collect any mercury residues precipitated inside the lamp housing by using an adsorbent available from a chemicals supplier. − Always disconnect the instrument from the line before each lamp exchange or any other work inside the instrument. 6 B 46-0063 e 03/06 Microscope Illuminating System HBO 100 1.7 Safety Disposal, Warranty Carl Zeiss Hints on Disposal Used HBO 103 lamps and defective microscope illuminators should not be treated like domestic waste; they should be disposed of in their original packaging in accordance with the legal regulations. When in doubt, please contact the Carl Zeiss microscopy service. 1.8 Hints on Warranty The instrument manufacturer is not liable for damage caused by unauthorized operation, particularly the removal and exchange of components, or the use of accessories from other manufacturers. This forfeits all the claims against warranty. B 46-0063 e 03/06 7 Instrument Description Components, Catalogue Numbers Carl Zeiss 2 2.1 Instrument Description Components of the Microscope Illuminating System Microscope Illuminating System HBO 100 The HBO 100 Microscope Illuminating System is available in a manually and a self-adjusting version. The microscope illuminating systems include the following components (Fig. 1/Fig. 2): − Illuminator with: dovetail for attachment to the microscope, integrated collector, heat-protection filter − HBO 103 W/2 mercury vapor short-arc lamp (Osram) − Transformer for HBO 100 − line cable with earth-contact plug or line cable with American flat plug HBO 103 1 2 3 4 Lamp, top part Line cable Transformer for HBO 100 Connecting cable Fig. 1 HBO 100 illuminator 423010 Microscope Illuminators HBO 100 illuminator, self-adjusting 423011-9901 5 Lamp, bottom part 6 Dovetail 7 Heat-protection filter HBO 100 Microscope Illuminating System (manual adjustment) Mercury vaporshort-arc lamp HBO 103 W/2 380301-9350-000 Lamp Transformer Line cable Transformer for HBO 100 432604-9901 Line cable with earth-contact plug 380137-6750-000 Line cable with American flat plug 380137-6740-000 Table: 1 2 3 4 Lamp, top part Line cable Transformer for HBO 100 Connecting cable Fig. 2 8 Catalogue numbers 5 Lamp, bottom part 6 Dovetail 7 Heat-protection filter HBO 100 Microscope Illuminating System, self-adjusting B 46-0063 e 03/06 Microscope Illuminating System HBO 100 2.2 Instrument Description Technical Data Carl Zeiss Technical Data of the Microscope Illuminating Systems Lamp housing HBO 100 illuminator Cat. No. 423010 HBO 100 illuminator, self-adjusting Cat. No. 423011-9901 Length 165 mm Width 166 mm Height 205 mm Connection to stand dovetail Connection to transformer Cable with special plug Transformer Transformer for HBO 100 Cat.No. 432604-9901 Line connection 100-240 V AC Line frequency 50-60 Hz Power consumption 155 VA Fuse F1/F2 2x T 2 A/H Lamp Mercury vapor short-arc lamp For the technical data of the lamps please see the information provided by the manufacturer. Recommended: Average life of the lamps B 46-0063 e 03/06 OSRAM Type HBO 103 W/2 Cat. No. 380301-9350-000 OSRAM Type HBO 100 W/2 Type HBO 103 W/2 200 h 300 h 9 Carl Zeiss Fig. 3 10 Instrument Description Technical Data, Line spectrum Microscope Illuminating System HBO 100 Line spectrum of the HBO 100/103 mercury short-arc lamps B 46-0063 e 03/06 Microscope Illuminating System HBO 100 Start-Up Lamp Insertion and Change 3 Start-Up 3.1 Lamp Insertion and Change Carl Zeiss − Before inserting the lamp, make sure the illuminating system has been disconnected from line power. − The lamps may only be changed when they are cooled down; allow the microscope illuminator to cool down for at least 15 minutes to avoid the risks of burns and explosion! − The lamp may only be removed from the packaging and inserted in the microscope illuminator if a protection mask and safety gloves are worn (see chapter 1.3). − All clamping adjustments must be performed carefully; strong heat during operation may result in loose contacts. 1 2 3 4 5 6 7 Fig. 4 Cooling body Fixation screws for top part of lamp Lamp mount Clamping lever at lamp base Control knob for collector Lamp Clamping lever at heat sink Inserting the HBO 103 lamp B 46-0063 e 03/06 11 Carl Zeiss Start-Up Lamp Insertion and Change Procedure: • Switch off the transformer. • Disconnect the microscope illuminator cable from the transformer. Microscope Illuminating System HBO 100 • Push clamping lever at lamp base (Fig. 4/4) down and insert the new lamp into the mount in the bottom part of the illuminator (as far as it will go); release the lever to clamp and center the lamp. • Allow the microscope illuminator to cool down for 15 minutes. • Wear protection mask and safety gloves. • Remove microscope illuminator with cooleddown lamp from the microscope. • Turn control knob (Fig. 4/5) to move collector to mid position. • Turn both fixation screws (Fig. 4/2) in the direction indicated by the arrow as far as it will go; remove top part of lamp upward. • Operate clamping lever (Fig. 4/7) at heat sink (Fig. 4/1) and withdraw heat sink upward from the safety holder (in commissioning) or from the lamp (Fig. 4/6). • Put heat sink down beside the illuminator. For this, you need not disconnect the electric connection to the heat sink. • Push clamping lever at lamp base (Fig. 4/4) down, to release the lamp lock; remove the defective lamp or the safety holder (Fig. 4/6) from the lamp mount (Fig. 4/3) in the bottom part of the illuminator. Release the lever. Do not exert any force when inserting the lamp. • Take the heat sink (Fig. 4/1), push down its clamping lever (Fig. 4/7) and put the heat sink onto the lamp (Fig. 4/6). Release the clamping lever. By doing so, the electric connection is established and the heat sink clamped. Do not exert any force to the lamp, when attaching the heat sink. • Put the top part of the illuminator onto the bottom part. • Turn both fastening screws (Fig. 4/2) on the bottom part of the illuminator oppositely to the direction indicated by the arrow as far as it will go. • Turn the control knob of the collector back to mid position, if necessary. • Dispose of the defective lamp in accordance with the relevant regulations (see section 1.7 – Hints on Disposal). 12 B 46-0063 e 03/06 Microscope Illuminating System HBO 100 3.2 Start-Up of HBO 100 Microscope Illuminating System Start-Up HBO 100 Carl Zeiss Do not look directly into the lamp or its reflected light. Procedure: • Install the HBO 100 microscope illuminator on the microscope (see Operating Manual of microscope). • Connect the microscope illuminator to the transformer as described in the supplied operating instructions: Fig. 5 • Connect the transformer to the power outlet using the provided power cable. 1 Connector for HBO 100 illuminator 2 Power connector Fig. 5 Rear of transformer for HBO 100 • Switch on the microscope illuminator using the power switch at the front of the transformer; the lamp will ignite automatically: Fig. 6 Function of the luminescence diodes: "POWER" Lights when instrument is switched on. "LAMP" Lights when the lamp has been ignited and is lighting. "TEMP" Lights when the temperature inside the transformer is within the permissible range. "SAFETY" Lights when the safety circuit of the lamp housing is closed. "+12V" Lights when the additional voltage of the transformer is within the permissible range. Operating hour meter/"Reset" key: 1 2 3 4 5 6 7 LED LAMP LED TEMP LED SAFETY LED +12V Reset key Display of operating hour meter POWER switch with integrated power indicator LED Fig. 6 Pressing the "Reset" key will reset the operating hour meter to "0". This must be done after every lamp change. To avoid resetting the meter by mistake, the key can be actuated only with an appropriate tool (e.g. a small screwdriver). Front of transformer for HBO 100 B 46-0063 e 03/06 13 Carl Zeiss 3.3 Start-Up Focusing and Centering Focusing and Centering the HBO 100 Microscope Illuminator (423010) Microscope Illuminating System HBO 100 Do not look directly into the lamp or its reflected light. • Switch on the lamp. Let it warm up for more than 5 minutes. • On the microscope, activate the adjusting aid. • Turn control knob of collector (Fig. 8/3) into mid position and generate a focused image of the lamp arc/mirror image (Fig. 7). Fig. 7 Lamp arc and mirror image Both images shall be – at about the same level and – closely side by side or half overlapping (Fig. 7) • If this is not the case, adjust lamp arc and mirror image accordingly by means of the screws for vertical (Fig. 8/1) and horizontal adjustment (Fig. 8/2). • Deactivate the adjusting aid again. 1 Screw for vertical adjustment 2 Screw for horizontal adjustment 3 Collector control knob Fig. 8 14 Adjusting elements of HBO 100 for manual adjustment B 46-0063 e 03/06 Microscope Illuminating System HBO 100 3.4 Start-Up Focusing and Centering Focusing and Centering the HBO 100 Microscope Illuminator, self-adjusting (423011-9901) Carl Zeiss Automatic adjustment of the HBO 100 requires the collector to be set to mid position. • Turn the collector control knob (Fig. 9/2) into mid position. After the transformer of the connected HBO 100 microscope illuminator was switched on, the five minutes warm-up phase begins. The LED key (Fig. 9/1) lights orange during warm-up. After the five minutes are over, automatic adjustment is started. The LED key flashes green during the adjustment. The adjustment is finished after about 40 seconds. The LED key lights permanently green then. If the adjustment failed, the LED key lights red. • In this case, you can start automatic adjustment manually by a short pressure (approx. one second) of the LED key (Fig. 9/1). 1 LED key 2 Collector control knob Fig. 9 Adjustment of HBO 100, self-adjusting B 46-0063 e 03/06 If the adjustment fails again, check whether the lamp has been inserted correctly in the base of the HBO 100 (pushed in as far as it will go). For this, refer to Section 3.1. 15 Carl Zeiss 3.5 Start-Up Setting Offset Values Setting Offset Values on HBO 100 Microscope Illuminator, self-adjusting (423011-9901) Microscope Illuminating System HBO 100 On the self-adjusting illuminator, the position of the lamp arc and its mirror image may be individually customized, if required, by the adjustment of offset values. After the automatic adjustment (see Section 3.4), LED key (Fig. 9/1) lights permanently green. By briefly pressing the recessed keys on the rear side of the illuminator housing (Fig. 10), the motors for arc positioning are moved in steps to the left, right, top or bottom within a limited range. During positioning, the LED key flashes alternately green and orange. If you do not press any key for more than 20 seconds, the currently set value will be stored. Not every key pressure will directly lead to a movement of the motors: The intensity change detected by the sensors must exceed a certain threshold, before the position is readjusted. Therefore, it may be necessary to press a key repeatedly. To reset the factory adjustment, the top button of the keypad must be pressed for ten seconds. Fig. 10 16 Keys for setting offset values for the adjustment of lamp arc and mirror image B 46-0063 e 03/06 Microscope Illuminating System HBO 100 3.6 Start-Up Inserting the Collector Attachment Inserting the Collector Attachment Carl Zeiss • Pull out clamping ring (Fig. 11/4). • Remove heat protection filter (Fig. 11/3). • Screw the collector attachment into the lamp as far as it will go (Fig. 11/1). The retaining ring on the collector attachment must point to the outside. • Clamp the collector attachment by inserting the spring ring (Fig. 11/2). The spring ring engages in the groove of the lamp. • Insert heat protection filter and lock it by means of the clamping ring. 1 2 3 4 Collector attachment Spring ring Heat protection filter Clamping ring Fig. 11 Inserting the collector attachment B 46-0063 e 03/06 17