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In this Guide...
This guide provides an optimized protocol for Illumina
paired-end multiplexed library preparation using the
SureSelectXT Library Prep and Capture System.
This guide describes the recommended operational
procedures to capture the genomic regions of interest using
biotinylated RNA oligomer libraries to enrich targeted
regions of the genome from repetitive sequences and
sequences unrelated to the research focus prior to sample
sequencing using the Illumina platform.
1
Before You Begin
This chapter contains information (such as procedural notes,
safety information, required reagents and equipment) that
you should read and understand before you start an
experiment.
2
Sample Preparation (3 µg DNA Samples)
This chapter describes the steps to prepare libraries for
target enrichment from 3-µg gDNA samples.
3
Sample Preparation (200 ng DNA Samples)
This chapter describes the steps to prepare libraries for
target enrichment from 200-ng gDNA samples.
4
Hybridization and Capture
This chapter describes the steps to hybridize and capture
the prepared library DNA.
5
Indexing and Sample Processing for Multiplexed Sequencing
This chapter describes the steps to amplify, purify, and
assess quality and quantity of the sample libraries. Samples
are pooled by mass prior to sequencing.
6
Reference
This chapter contains reference information, including
component kit contents and index sequences.
SureSelectXT Target Enrichment System for Illumina Multiplexed Sequencing
3