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2 Chapter 2 Fragment Library Preparation Overview Overview This chapter describes the method to generate a small-insert library (150 to 180 bp, before adaptor ligation). This method involves shearing DNA into small fragments and ligating P1 and P2 Adaptors (see Figure 2 and Figure 3). 14 Figure 2 Basic fragment library preparation workflow overview. Figure 3 Fragment library design. SOLiD™ 4 System Library Preparation Guide
