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C Appendix C Supplemental Procedures PAGE gel DNA elution PAGE gel DNA elution Materials and equipment required Table 99 Required equipment Item ‡ Source Microcentrifuge 5417R, refrigerated, without rotor • Eppendorf § 022621807 (120 V/60 Hz) • Eppendorf‡ 022621840 (230 V/50 Hz)) FA-45-24-11, fixed-angle rotor 24 × 1.5/2 mL, including aluminum lid, aerosol-tight Eppendorf Pipettors Major Laboratory Supplier (MLS) 022636006 ‡ Applied Biosystems has validated this protocol using this specific material. Substitution may adversely affect system performance. § Or equivalent but validation of the equipment for library preparation is required. Table 100 Required consumables Item ‡ TE, pH 8.0 Source Applied Biosystems PN AM9858 7.5 M Ammonium acetate Sigma-Aldrich A-2706 100✕ BSA New England, Inc. B9001S 0.45 μm Nanosep® spin columns VWR ODM45C34 0.5-mL LoBind Tubes Eppendorf 022431005 1.5-mL LoBind Tubes Eppendorf 022431021 21-gauge needle Major Laboratory Supplier (MLS) Razor blades MLS Filtered pipettor tips MLS ‡ Applied Biosystems has validated this protocol using this specific material. Substitution may adversely affect system performance. 196 SOLiD™ 4 System Library Preparation Guide
